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Mouse Integrin alpha-M (Itgam/CD11b) ELISA Kit

  • 中文名称:
    小鼠整合素αM(Itgam/CD11b)酶联免疫试剂盒
  • 货号:
    CSB-E17417m
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:

    This Mouse Itgam/CD11b ELISA Kit was designed for the quantitative measurement of Mouse Itgam/CD11b protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 别名:
    Itgam ELISA Kit; Integrin alpha-M ELISA Kit; CD11 antigen-like family member B ELISA Kit; CR-3 alpha chain ELISA Kit; Cell surface glycoprotein MAC-1 subunit alpha ELISA Kit; Leukocyte adhesion receptor MO1 ELISA Kit; CD antigen CD11b ELISA Kit
  • 缩写:
    Itgam/CD11b
  • Uniprot No.:
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围:
    15.6 pg/mL-1000 pg/mL
  • 灵敏度:
    3.9 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse Itgam/CD11b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:100 Average % 86
    Range % 81-90
    1:200 Average % 103
    Range % 99-108
    1:400 Average % 97
    Range % 92-101
    1:800 Average % 90
    Range % 86-94
  • 回收率:
    The recovery of mouse Itgam/CD11b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 90-100
    EDTA plasma (n=4) 88 84-92
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1000 2.603 2.527 2.565 2.376
    500 1.920 1.865 1.893 1.704
    250 1.367 1.441 1.404 1.215
    125 0.905 0.928 0.917 0.728
    62.5 0.586 0.574 0.580 0.391
    31.2 0.439 0.419 0.429 0.240
    15.6 0.291 0.304 0.298 0.109
    0 0.186 0.192 0.189  
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

靶点详情

  • 功能:
    Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils. May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development. Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development.
  • 亚细胞定位:
    Cell membrane; Single-pass type I membrane protein. Membrane raft; Single-pass type I membrane protein.
  • 蛋白家族:
    Integrin alpha chain family
  • 组织特异性:
    Predominantly expressed in monocytes and granulocytes. Expressed in a subset of peritoneal mast cells. Expressed in microglia (at protein level).
  • 数据库链接: