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  4. Mouse Insulin-like peptide INSL5(INSL5) ELISA kit

Mouse Insulin-like peptide INSL5(INSL5) ELISA kit

  • 中文名称：
    小鼠胰岛素样肽INSL5(INSL5)酶联免疫试剂盒
  • 货号：
    CSB-EL011749MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse INSL5 ELISA Kit was designed for the quantitative measurement of Mouse INSL5 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.

  • 别名：
    Insl5 ELISA Kit; Rif ELISA Kit; Rif2 ELISA Kit; Zins3 ELISA Kit; Insulin-like peptide INSL5 ELISA Kit; Insulin-like peptide 5 ELISA Kit; Relaxin/insulin-like factor 2 ELISA Kit; Relaxin/insulin-like protein) [Cleaved into: Insulin-like peptide INSL5 B chain; Insulin-like peptide INSL5 A chain] ELISA Kit
  • 缩写：
    INSL5
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    3.12 pg/mL-200 pg/mL
  • 灵敏度：
    0.78 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse INSL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:100Average %93
    Range %90-96
    1:200Average %100
    Range %97-103
    1:400Average %88
    Range %85-93
    1:800Average %107
    Range %103-110
  • 回收率：
    The recovery of mouse INSL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8481-89
    EDTA plasma (n=4)9893-102
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.417 2.446 2.432 2.262
    1002.046 2.008 2.027 1.857
    501.562 1.568 1.565 1.395
    251.017 1.035 1.026 0.856
    12.50.692 0.669 0.681 0.511
    6.250.425 0.435 0.430 0.260
    3.120.306 0.330 0.318 0.148
    00.169 0.171 0.170
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    May have a role in gut contractility or in thymic development and regulation. Activates RXFP4 with high potency and appears to be the endogenous ligand for this receptor.
  • 基因功能参考文献：
    1. Mass spectral analysis of purified enteroendocrine cells and tissue homogenates identified the exact sequence of A and B chains of INSL5 endogenously expressed in L-cells. PMID: 28857318
    2. Insl5 is an orexigenic hormone released from colonic L-cells, which promotes appetite during conditions of energy deprivation. PMID: 25028498
    3. INSL5 may be a unique marker of colorectal enteroendocrine cells, and INSL5-RXFP4 signaling might play a role in an autocrine/paracrine fashion in the colorectal epithelium and rectal neuroendocrine tumors. PMID: 23438439
    4. Insl5 knockout mice displayed impaired male and female fertility that are due to marked reduction in sperm motility and irregular length of the estrous cycle. Also impaired glucose homeostasis due to glucose intolerance. PMID: 22822165
    5. Insulin-like peptide 5 is expressed in mouse brain and has a role in mobilization of calcium PMID: 16601133

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Insulin family
  • 组织特异性：
    Highest expression in colon with lower levels in thymus. Minimal levels in testis.
  • 数据库链接：