Mouse Insulin-like growth factor-binding protein complex acid labile subunit(IGFALS) ELISA kit
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中文名称:小鼠胰岛素样生长因子结合蛋白复合物酸不稳定亚基(IGFALS)酶联免疫试剂盒
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货号:CSB-EL011094MO
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规格:96T/48T
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价格:¥3600/¥2500
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其他:
产品详情
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产品描述:
This Mouse IGFALS ELISA Kit was designed for the quantitative measurement of Mouse IGFALS protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.
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别名:Igfals ELISA Kit; Albs ELISA Kit; AlsInsulin-like growth factor-binding protein complex acid labile subunit ELISA Kit; ALS ELISA Kit
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缩写:IGFALS
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Uniprot No.:
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种属:Mus musculus (Mouse)
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样本类型:serum, plasma, tissue homogenates
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检测范围:3.12 ng/mL-200 ng/mL
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灵敏度:0.78 ng/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Others
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IGFALS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 96 Range % 93-99 1:200 Average % 84 Range % 80-87 1:400 Average % 91 Range % 87-94 1:800 Average % 107 Range % 104-110 -
回收率:
The recovery of mouse IGFALS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 90-98 EDTA plasma (n=4) 85 81-89 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 200 1.985 1.901 1.943 1.838 100 1.543 1.578 1.561 1.456 50 1.055 1.064 1.060 0.955 25 0.657 0.648 0.653 0.548 12.5 0.346 0.352 0.349 0.244 6.25 0.248 0.268 0.258 0.153 3.12 0.191 0.193 0.192 0.087 0 0.106 0.104 0.105 -
数据处理:
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货期:3-5 working days
引用文献
- Tmem263 deletion disrupts the GH/IGF-1 axis and causes dwarfism and impairs skeletal acquisition DC Sarver,/,2024
- Tmem263 deletion disrupts the GH/IGF-1 axis and causes dwarfism and impairs skeletal acquisition DC Sarver,bioRxiv,2023
- First use of gene therapy to treat growth hormone resistant dwarfism in a mouse model KC Sia,Gene Ther,2022
相关产品
靶点详情
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功能:May have an important role in regulating the access of circulating IGFs to the tissues.
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基因功能参考文献:
- the Igfals gene has a role in skeletal response of male mice to anabolic hormone therapy PMID: 24424061
- A modest reduction in post-natal growth in the null ALS mice and in the ALS-deficient patients was observed PMID: 16114275
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亚细胞定位:Secreted, extracellular space.
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数据库链接:
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