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  4. Mouse Insulin-like growth factor binding protein 4,IGFBP-4 ELISA kit

Mouse Insulin-like growth factor binding protein 4,IGFBP-4 ELISA kit

  • 中文名称：
    小鼠胰岛素样生长因子结合蛋白4(IGFBP-4)酶联免疫试剂盒
  • 货号：
    CSB-E07355m
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse IGFBP4 ELISA Kit was designed for the quantitative measurement of Mouse IGFBP4 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL.

  • 别名：
    Igfbp4 ELISA Kit; Igfbp-4 ELISA Kit; Insulin-like growth factor-binding protein 4 ELISA Kit; IBP-4 ELISA Kit; IGF-binding protein 4 ELISA Kit; IGFBP-4 ELISA Kit
  • 缩写：
    IGFBP4
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma
  • 检测范围：
    0.78 ng/mL-50 ng/mL
  • 灵敏度：
    0.195 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IGFBP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 92    
    Range % 88-96    
    1:2 Average % 84    
    Range % 80-88    
    1:4 Average % 97    
    Range % 92-101    
    1:8 Average % 105    
    Range % 100-112    
  • 回收率：
    The recovery of mouse IGFBP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 90-100    
    EDTA plasma (n=4) 88 84-92    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    50 2.384 2.495 2.440 2.290    
    25 2.041 2.103 2.072 1.922    
    12.5 1.605 1.569 1.587 1.437    
    6.25 1.002 0.981 0.992 0.842    
    3.12 0.519 0.538 0.529 0.379    
    1.56 0.339 0.352 0.346 0.196    
    0.78 0.263 0.274 0.269 0.119    
    0 0.152 0.147 0.150 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • 基因功能参考文献：
    1. Igfbp4 is required for inguinal fat expansion in female mice but not in male mice. However, gonadal WAT expansion, which is prevented by estrogen during high-fat diet, does not require Igfbp4. PMID: 28938423
    2. IGFBP-4 modulates the skeleton in a gender-specific manner, acting as both a cell autonomous and cell non-autonomous factor. PMID: 28184001
    3. exerts a negative effect on basal intestinal growth but plays a positive regulatory role in the intestinotropic actions of GLP-2 PMID: 25514089
    4. IGF-1/IGFbp4 signaling regulated the post-developmental adipose tissue expansion. PMID: 24778188
    5. SOX9 is a transcriptional regulator of IGFBP-4 and that SOX9-induced activation of IGFBP-4 may be one of the mechanisms by which SOX9 suppresses cell proliferation and progression of colon cancer. PMID: 23660500
    6. findings indicate that the defect in p53 function and the increased proteolysis of IGFBP-4, we had observed, represent two components of the same pathway, which contributes to the oncogenic function of Skp2B PMID: 21829624
    7. Inactivation of IGFBP4 diminished the anabolic effects of PAPP-A on bone. PMID: 20144555
    8. Targeted expression of a protease-resistant IGFBP-4 mutant in smooth muscle of transgenic mice PMID: 11923290
    9. IGFBP-4 is a potential growth inhibitor of lymphoid tissues. PMID: 15072544
    10. This study demonstrates that ES of muscle cells in vitro not only directly modulates the gene expression of contractile proteins but also modulates proteins that are part of the IGF regulatory system, in particular IGFBP-4. PMID: 15584093
    11. Crystallization, heparin and IGF binding, and osteogenic activity of IGFBP4 in its native conformation PMID: 15735305
    12. Overexpression of Hbegf in transgenic mice downregulates Igfbp4. PMID: 16019424
    13. IGFBP-4 may have a role in increasing necrosis and apoptosis, but in decreasing mitosis PMID: 17824980
    14. Results indicate that IGFBP-4 functions as a local reservoir to optimize IGF-II actions needed for normal embryogenesis, and also establish that IGFBP-4 proteolysis is required to activate most, if not all, IGF-II mediated growth-promoting activity. PMID: 18258685
    15. IGFBP-4 is an inhibitor of the canonical Wnt signalling required for cardiogenesis and provides a molecular link between insulin-like growth factor signalling and Wnt signalling PMID: 18528331
    16. SOD1(G93A) mutant transgenic mice as a model of amyotrophic lateral sclerosis (ALS) and immunohistochemical studies to investigate the changes of insulin-like growth factor-binding protein 4 (IGFBP4) in the central nervous system. PMID: 18823765

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  • 亚细胞定位：
    Secreted.
  • 数据库链接：