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  4. Mouse Hydroxyacid oxidase 1(HAO1) ELISA kit

Mouse Hydroxyacid oxidase 1(HAO1) ELISA kit

  • 中文名称：
    小鼠羟基酸氧化酶1(HAO1)酶联免疫试剂盒
  • 货号：
    CSB-EL010127MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse HAO1 ELISA Kit was designed for the quantitative measurement of Mouse HAO1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

  • 别名：
    Hao1 ELISA Kit; Gox1 ELISA Kit; Hao-1 ELISA Kit; Hydroxyacid oxidase 1 ELISA Kit; HAOX1 ELISA Kit; EC 1.1.3.15 ELISA Kit; Glycolate oxidase ELISA Kit; GOX ELISA Kit
  • 缩写：
    HAO1
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    6.25 pg/mL-400 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse HAO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %94
    Range %83-107
    1:2Average %95
    Range %89-103
    1:4Average %92
    Range %85-105
    1:8Average %91
    Range %87-99
  • 回收率：
    The recovery of mouse HAO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9892-103
    EDTA plasma (n=4)9690-104
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.413 2.347 2.380 2.198
    2001.603 1.589 1.596 1.414
    1000.902 0.865 0.884 0.702
    500.549 0.527 0.538 0.356
    250.388 0.363 0.376 0.194
    12.50.282 0.272 0.277 0.095
    6.250.253 0.237 0.245 0.063
    00.180 0.183 0.182
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate.
  • 亚细胞定位：
    Peroxisome.
  • 蛋白家族：
    FMN-dependent alpha-hydroxy acid dehydrogenase family
  • 组织特异性：
    Liver.
  • 数据库链接：