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  4. Mouse Glia-derived nexin(SERPINE2) ELISA kit

Mouse Glia-derived nexin(SERPINE2) ELISA kit

  • 中文名称：
    小鼠胶质细胞源性连接蛋白(SERPINE2)酶联免疫试剂盒
  • 货号：
    CSB-EL021082MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse SERPINE2 ELISA Kit was designed for the quantitative measurement of Mouse SERPINE2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.

  • 别名：
    Serpine2 ELISA Kit; Pi7 ELISA Kit; Pn1 ELISA Kit; Spi4Glia-derived nexin ELISA Kit; GDN ELISA Kit; Peptidase inhibitor 7 ELISA Kit; PI-7 ELISA Kit; Protease nexin 1 ELISA Kit; PN-1 ELISA Kit; Protease nexin I ELISA Kit; Serine protease-inhibitor 4 ELISA Kit; Serpin E2 ELISA Kit
  • 缩写：
    SERPINE2
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.12 pg/mL-200 pg/mL
  • 灵敏度：
    0.78 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SERPINE2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:100 Average % 103
    Range % 99-112
    1:200 Average % 84
    Range % 80-88
    1:400 Average % 97
    Range % 92-101
    1:800 Average % 90
    Range % 85-94
  • 回收率：
    The recovery of mouse SERPINE2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 89 85-93
    EDTA plasma (n=4) 95 90-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    200 2.185 2.312 2.249 2.111
    100 1.841 1.937 1.889 1.751
    50 1.437 1.385 1.411 1.273
    25 0.946 1.002 0.974 0.836
    12.5 0.598 0.579 0.589 0.451
    6.25 0.362 0.374 0.368 0.230
    3.12 0.235 0.226 0.231 0.093
    0 0.134 0.141 0.138 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin.
  • 基因功能参考文献：
    1. SerpinE2 is required in the extracellular milieu of breast tumors where it acts in multiple ways to regulate tumor matrix deposition, thereby controlling tumor cell dissemination. PMID: 27793045
    2. These data uncover a novel mechanism whereby loss of serine protease inhibition leads to lung lymphocyte accumulation. PMID: 27059719
    3. the involvement of PAI-1 in HIF-1-enhanced thrombosis and that an additional factor participates in regulating Pai-1 expression in compressed skin. PMID: 25939592
    4. aberrant Serpine2/Pn-1 is required for proliferation of human and mouse medulloblastoma cells. PMID: 25901736
    5. PN-1 strongly limits physiological angiogenesis. PMID: 26109427
    6. The PAI-1 inhibitor not only accelerated the expansion of the donor HSCs during the early-stage of regeneration, but also supported long-term hematopoiesis. PMID: 24155177
    7. a novel inhibitory interaction between CAP1/Prss8 and nexin-1, opening the search for specific CAP1/Prss8 antagonists that are independent of its catalytic activity PMID: 25138159
    8. SERPINE2 and PLAU are involved in cumulus expansion and oocyte maturation. PMID: 24023701
    9. Endothelial protease nexin-1 is a novel regulator of A disintegrin and metalloproteinase 17 maturation and endothelial protein C receptor shedding via furin inhibition. PMID: 23661674
    10. PN-1 has direct antiangiogenic properties and is a yet-unrecognized player in the angiogenic balance PMID: 22331468
    11. SERPINE2 protein may play a role as a sperm decapacitation factor. PMID: 21084713
    12. platelet PN-1 can be considered as a new important regulator of thrombolysis in vivo PMID: 21403095
    13. SERPINE2 is a major plasminogen activator(PA) inhibitor in the placenta and uterus during the estrous cycle, pregnancy, and lactation. It may participate in the PA-modulated tissue remodeling process in the mouse placenta and uterus. PMID: 20977773
    14. Data suggest that lack of extinction in protease nexin-1 KO mice is associated with changes in neuronal activity in the circuitry of the basal and central amygdaloid nuclei, supporting a differential impact on fear extinction in these areas. PMID: 20529116
    15. Platelet PN-1 is a key player in the thrombotic process, whose negative regulatory role has been, up to now, markedly underestimated. PMID: 19855083
    16. PN-1 may have a role in regulating activities of muscle proteases PMID: 14733957
    17. We report that brain proteolytic activity was strongly regulated by neuronal activity, with PN-1 being a major controlling element. PMID: 15509762
    18. p65 binds to the kappaB-like DNA sequence located at approximately -9 kbp in the SerpinE2 PMID: 16697375
    19. Expression array analysis in murine models demonstrated association and repication of SERPINE2 as a potential COPD susceptibility gene. PMID: 16921128
    20. Results indicate that PN-1 could have a natriuretic role by inhibiting prostasin activity. Aldosterone and TGF-beta reciprocally regulate the expression of PN-1. PN-1 could represent a new factor that contributes to regulation of ENaC activity. PMID: 16941024
    21. Schwann cell-derived protease nexin-1 is crucial for proper reinervation and cell survival. PMID: 17409231
    22. PN-1 is upregulated in cryoglobulinemic membranoproliferative glomerulonephritis PMID: 18199802
    23. Results suggest that PN-1 might become a prognostic marker in breast cancer. PMID: 19584287

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  • 亚细胞定位：
    Secreted, extracellular space.
  • 蛋白家族：
    Serpin family
  • 组织特异性：
    Most abundant in seminal vesicles.
  • 数据库链接：

    KEGG: mmu:20720

    STRING: 10090.ENSMUSP00000027467

    UniGene: Mm.3093