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Mouse Galanin-like peptide(GALP) ELISA kit

  • 中文名称：
    小鼠甘丙肽样肽(GALP)酶联免疫试剂盒
  • 货号：
    CSB-EL009222MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse GALP ELISA Kit was designed for the quantitative measurement of Mouse GALP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

  • 别名：
    GalpGalanin-like peptide ELISA Kit
  • 缩写：
    GALP
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    23.5 pg/mL-1500 pg/mL
  • 灵敏度：
    5.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse GALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 93
    Range % 86-97
    1:2 Average % 104
    Range % 97-108
    1:4 Average % 92
    Range % 87-98
    1:8 Average % 91
    Range % 87-95
  • 回收率：
    The recovery of mouse GALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 87 83-92
    EDTA plasma (n=4) 94 87-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1500 2.421 2.536 2.479 2.288
    750 1.905 2.011 1.958 1.767
    375 1.328 1.413 1.371 1.180
    187.5 0.821 0.834 0.828 0.637
    94 0.514 0.527 0.521 0.330
    47 0.370 0.387 0.379 0.188
    23.5 0.311 0.315 0.313 0.122
    0 0.190 0.192 0.191 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Hypothalamic neuropeptide which binds to the G-protein-coupled galanin receptors (GALR1, GALR2 and GALR3). Involved in a large number of putative physiological functions in CNS homeostatic processes, including the regulation of gonadotropin-releasing hormone secretion.; Exhibits antimicrobial activity against Gram-negative bacterias, inducing bacterial membrane blebbing. Exhibits potent and dose-dependent vasoconstrictor and anti-edema activity in the cutaneous microvasculature, a physiologic effects which does not appear to be mediated via GALR1 or GALR2.
  • 基因功能参考文献：
    1. this study demonstrates anti-obesity effect of intranasal administration of galanin-like peptide in obese mice PMID: 27323911
    2. we investigated whether PI3K signalling in neurones expressing GALP plays a role in the transcriptional regulation of the GALP gene PMID: 24796383
    3. results indicate the GALP ICV administration can further promote energy metabolism when administered to spontaneously exercising mice PMID: 24055807
    4. Distinct expression of alarin, a splice variant of GALP, in the adult mouse brain demonstrates potential functions in reproduction and metabolism. PMID: 21647713
    5. hypothalamic GALP gene expression is controlled by leptin signals PMID: 12746327
    6. GALP may play a role in readjusting energy balance under changing nutritional circumstances PMID: 18775887

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Galanin family
  • 组织特异性：
    Isoform 2 is found in brain, thymus and skin. Isoform 2 is found in the skin, in pericytes covering microvascular arterioles and venules on their abluminal surfaces. In larger vessels, isoform 2 is expressed in layers of smooth muscle cells. Isoform 2 is
  • 数据库链接：