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Mouse Ficolin-2(FCN2) ELISA kit

  • 中文名称：
    小鼠纤维胶凝蛋白-2(FCN2)酶联免疫试剂盒
  • 货号：
    CSB-EL008551MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse FCN2 ELISA Kit was designed for the quantitative measurement of Mouse FCN2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL.

  • 别名：
    Fcn2 ELISA Kit; FcnbFicolin-2 ELISA Kit; Collagen/fibrinogen domain-containing protein 2 ELISA Kit; Ficolin-B ELISA Kit; Ficolin-beta ELISA Kit; L-ficolin ELISA Kit
  • 缩写：
    FCN2
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    125 pg/mL-8000 pg/mL
  • 灵敏度：
    31.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse FCN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1000 Average % 92
    Range % 88-96
    1:2000 Average % 94
    Range % 90-98
    1:4000 Average % 95
    Range % 90-100
    1:8000 Average % 88
    Range % 83-93
  • 回收率：
    The recovery of mouse FCN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 84 80-89
    EDTA plasma (n=4) 96 92-101
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    8000 2.495 2.569 2.532 2.385
    4000 2.014 2.071 2.043 1.896
    2000 1.622 1.584 1.603 1.456
    1000 1.178 1.113 1.146 0.999
    500 0.731 0.792 0.762 0.615
    250 0.456 0.428 0.442 0.295
    125 0.345 0.327 0.336 0.189
    0 0.147 0.146 0.147 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc-binding lectin.
  • 基因功能参考文献：
    1. this study shows that FCN B plays an important role in the development of collagen Ab-induced arthritis PMID: 28739878
    2. Ficolin-2 defends against virulent Mycobacteria tuberculosis infection in vivo, and its insufficiency is associated with infection in humans. PMID: 24040095
    3. Data show that ficolin-B is stored in and set free from immature granulocytic myeloid cells indicating a role in the early infection-induced cellular response of these inflammatory cells. PMID: 23911405
    4. These results identify mouse ficolin-B as a functional member of the ficolin family activating complement via the lectin pathway. PMID: 22459270
    5. Results show that ficolin B in mouse bone marrow is an oligomeric protein. Ficolin B contained very low, but detectable, amounts of MASP-2 and sMAP and showed detectable C4-deposition activity on immobilized N-acetylglucosamine. PMID: 22523468
    6. Ficolin B exhibited a distinct ontogeny pattern that switched from embryonic liver to postnatal bone marrow and spleen. The cells that express ficolin B mRNA were identified as belonging to the myeloid cell lineage. PMID: 15950722
    7. Ficolin B showed multimeric structures and revealed binding to both N-acetylglucosamine and N-acetylgalactosamine. Ficolin B specifically recognized sialic acid residues. Ficolin B plays a distinct role through its unique carbohydrate-binding specificity. PMID: 16328467

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Ficolin lectin family
  • 数据库链接：