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  4. Mouse Ezrin(EZR) ELISA kit

Mouse Ezrin(EZR) ELISA kit

  • 中文名称：
    小鼠埃兹蛋白(EZR)酶联免疫试剂盒
  • 货号：
    CSB-EL007914MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse EZR ELISA Kit was designed for the quantitative measurement of Mouse EZR protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

  • 别名：
    Ezr ELISA kit; Vil2Ezrin ELISA kit; Cytovillin ELISA kit; Villin-2 ELISA kit; p81 ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse EZR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 86
    Range % 80-91
    1:2 Average % 96
    Range % 91-102
    1:4 Average % 103
    Range % 97-108
    1:8 Average % 93
    Range % 89-97
  • 回收率：
    The recovery of mouse EZR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 96 92-102
    EDTA plasma (n=4) 87 80-92
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    4000 2.412 2.526 2.469 2.284
    2000 2.113 2.148 2.131 1.946
    1000 1.632 1.562 1.597 1.412
    500 0.978 0.996 0.987 0.802
    250 0.546 0.531 0.539 0.354
    125 0.369 0.361 0.365 0.180
    62.5 0.268 0.274 0.271 0.086
    0 0.186 0.184 0.185 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
  • 基因功能参考文献：
    1. Ezrin level may serve as a differential diagnostic and/or prognostic biomarker for high and low lymphatic metastasis -Hepatocellular carcinoma (HCC)and may be beneficial in the diagnosis of HCC disease PMID: 28608644
    2. Inactivation of SHIP2 leads to increased microvilli formation and solute reabsorption by the renal proximal tubule and was associated with hyperactivated ezrin/radixin/moesin proteins and increased Rho-GTP. PMID: 28302370
    3. high actin-ezrin intensity area restricts the lateral movement of B cell receptors upon stimulation, reducing receptor clustering and signaling PMID: 28821013
    4. Ezrin may play a role in regulating lymphatic metastasis in hepatocellular carcinoma and might be inversely associated with A7 expression. PMID: 27914826
    5. Augmented hypertension-induced glomerular capillary injury in mice lacking CLIC5 results from abrogation of Rac1-dependent Pak and ezrin activation, perhaps reducing the tensile strength of the podocyte actin cytoskeleton. PMID: 26924049
    6. Data, including data from studies in knockout mice, suggest that VDR (vitamin D receptor) regulates expression of ezrin in enterocytes; VDR appears not to be involved in morphology of tight junctions and absorption of large molecules in enterocytes. PMID: 26606857
    7. Lack of ezrin not only causes achlorhydria and hypergastrinemia but also changes the structure of gastric glands, with severe perturbation of the secretory membranes of parietal cells. PMID: 26329936
    8. our study demonstrates that ezrin is a novel regulator of IL-10 production by B cells PMID: 26673134
    9. Merlin and Ezrin are components of a mechanism where mechanical forces associated with cell junctions are transduced across the cell cortex via cortical actomyosin cytoskeleton to control lateral mobility and activity of epidermal growth factor receptor. PMID: 26483553
    10. Dysfunction of ezrin mimics important aspects of the pathological mechanisms responsible for cholangiopathies PMID: 25311759
    11. We hypothesize that polyvalent electrostatic interactions are responsible for the assembly of CD44 clusters and the multimeric PIP2-CD44-Ezrin complexes. PMID: 25572402
    12. These results indicate that ezrin is required for uptake of hypotaurine from maternal serum by placental trophoblasts, and plays an important role in fetal growth. PMID: 25144766
    13. These data altogether suggest a novel role of ezrin in the neuritogenesis of the cultured cortical neurons through down-regulation of RhoA activity. PMID: 25144196
    14. Beta-dystroglycan can respond to ezrin driven cytoskeletal and cell morphology changes,by translocating from the cytoplasm to the nucleus. PMID: 24599031
    15. The findings demonstrate that adult neural stem cells and neuroblasts express ezrin and that ezrin may be involved in intracellular actin remodeling. PMID: 23229862
    16. ALCAM stably interacts with actin by binding to syntenin-1 and ezrin. PMID: 24662291
    17. Ezrin binds to and is dephosphorylated by PTPsigma in vitro, suggesting it is a direct PTPsigma substrate. PMID: 24385580
    18. Data indicate that Crb3 (Crumbs3) is crucial for epithelial morphogenesis and plays a role in linking the apical membrane to the underlying ezrin-containing cytoskeleton. PMID: 24164893
    19. ezrin regulates amplification of b-cell receptor signals and tunes the strength of B cell activation and humoral immunity. PMID: 24043890
    20. Ezrin and moesin are required for efficient T cell adhesion and homing to lymphoid organs. PMID: 23468835
    21. Phosphoproteome analyses reveal specific implications of Hcls1, p21-activated kinase 1 and Ezrin in proliferation of a myeloid progenitor cell line downstream of wild-type and ITD mutant Fms-like tyrosine kinase 3 receptors. PMID: 23017497
    22. Ezrin, a membrane cytoskeletal cross-linker, is essential for the regulation of phosphate and calcium homeostasis. PMID: 22895514
    23. during 8-cell embryocompaction Rho GTPases facilitate the apical distribution of aPKC and Ezrin; aPKC and Mek/Erk work together to promote Ezrin phosphorylation at the apical region; data also suggested that aPKC might be the Ezrin kinase during eight-cell embryo compaction PMID: 23313818
    24. Ezrin has a novel role as a spatial adaptor that couples JNK signaling components to the BCR signalosome, thus facilitating JNK activation. PMID: 23338238
    25. A Pak1-PP2A-ERM signaling axis mediates F-actin rearrangement and degranulation in mast cells. PMID: 23063725
    26. the extracellular matrix molecule VN and its neuronal receptor TLCN play a pivotal role in the phosphorylation of ezrin/radixin/moesin proteins and the formation of phagocytic cup-like structures on neuronal dendrites PMID: 23019340
    27. Ezrin/radixin/moesin are required for the purinergic P2X7 receptor (P2X7R)-dependent processing of the amyloid precursor protein. PMID: 22891241
    28. our results suggest that dynamic regulation of Ezrin phosphorylation at amino acid T567 that controls structural transitions of this protein plays a pivotal role in tumor progression and metastasis, possibly in part by altering cellular metabolism. PMID: 22147261
    29. The ERM (ezrin, radixin, moesin) proteins are novel scaffolds at the level of SOS activity control, which is relevant for both normal Ras function and dysfunction known to occur in several human cancers. PMID: 22132106
    30. Ezrin-radixin-moesin (ERM) proteins are induced in activated microglia/macrophages, whereas ERM molecules are only marginally expressed in quiescent microglia in the normal brain. PMID: 21451358
    31. Ezrin is key regulator of Src-induced malignant phenotype in three-dimensional environment. PMID: 21666723
    32. Ezrin, radixin, and moesin are phosphorylated in response to 2-methoxyestradiol and modulate endothelial hyperpermeability. PMID: 21659656
    33. Dbl is regulated by hamartin through association with ezrin. PMID: 21712385
    34. The function of Ezrin in building and maintaining the apical domain is essential not only for intestinal morphogenesis but also for homeostasis in the mature intestine. PMID: 21730140
    35. deletion of ezrin results in defects in mouse intestinal villus morphogenesis. (Review) PMID: 21343695
    36. Transient dephosphorylation of ezrin regulates membrane flexibility that the B cell must achieve during migration, followed by relocalization to the actin-rich lamellipodia for dynamic forward protrusion of the cells. PMID: 21339367
    37. Data suggest that myosin-II and ERM proteins modulate mechanical properties in oocytes, contributing to cell polarity and to completion of meiosis. PMID: 20660156
    38. ezrin is dispensable for lymphoid development PMID: 20806059
    39. CLIC5A colocalizes with ezrin and podocalyxin in podocytes and is required for the development/maintenance of the proper glomerular endothelial cell and podocyte architecture. PMID: 20335315
    40. Data show that ezrin associates with Hsc70 that locates on the membrane of TG cells and participates in the bacterial uptake by TG cells. PMID: 19737422
    41. Ezrin and EBP50 interact with high affinity; disruption of this complex interfered with cAMP modulation of T-cell activation PMID: 19857202
    42. Results suggest that if mutations that contribute to polycystic kidney disease pathogenesis might disrupt normal tubular development, the mechanism may include altered ezrin function and abnormal protein kinase A targeting. PMID: 12840161
    43. cross talk between the blastocyst and uterus involving the ezrin/radixin/moesin (ERM) proteins and ERM-associated cytoskeletal cross-linker proteins CD43, CD44, ICAM-1, and ICAM-2 PMID: 14613898
    44. Data show that ezrin and the homeodomain-containing transcription factor Six-1 (sine oculis-related homeobox-1 homolog) had essential roles in determining the metastatic fate of rhabdomyosarcoma cells. PMID: 14704789
    45. in a mouse model of osteosarcoma ezrin was found to be necessary for metastasis; results showed that ezrin expression provided an early survival advantage for cancer cells that reached the lung PMID: 14704791
    46. Ezrin organizes the apical terminal web region of intestinal epithelium, which is critical the process of de novo lumen formation and expansion during villus morphogenesis. PMID: 15177033
    47. Ezrin gene, coding for a membrane-cytoskeleton linker protein, is regionally expressed in the developing mouse neuroepithelium PMID: 15465499
    48. Adult Vil2(kd/kd) mice suffered from severe achlorhydria. PMID: 15809309
    49. ezrin is required for breast cancer metastasis PMID: 15987432
    50. ezrin and beta-catenin are both present at the cell membrane of the glial tube cells suggests that these proteins may be involved in those signaling processes. PMID: 16996217

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  • 亚细胞定位：
    Apical cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection. Cell projection, microvillus membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cell projection, microvillus.
  • 组织特异性：
    Detected in eye lens fiber cells. Expressed in cerebrum and cerebellum (at protein level). Component of the microvilli of intestinal epithelial cells.
  • 数据库链接：