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Mouse Epidermal growth factor-like protein 6(EGFL6) ELISA kit

  • 中文名称:
    小鼠表皮生长因子样蛋白6(EGFL6)酶联免疫试剂盒
  • 货号:
    CSB-EL007475MO
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:

    This Mouse EGFL6 ELISA Kit was designed for the quantitative measurement of Mouse EGFL6 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 93.75 pg/mL-6000 pg/mL and the sensitivity is 23.43 pg/mL.

  • 别名:
    Egfl6 ELISA Kit; Maeg ELISA Kit; Epidermal growth factor-like protein 6 ELISA Kit; EGF-like protein 6 ELISA Kit; MAM and EGF domains-containing gene protein ELISA Kit; Protein W80 ELISA Kit
  • 缩写:
    EGFL6
  • Uniprot No.:
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    93.75 pg/mL-6000 pg/mL
  • 灵敏度:
    23.43 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse EGFL6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %90
    Range %85-94
    1:2Average %98
    Range %94-101
    1:4Average %102
    Range %94-108
    1:8Average %94
    Range %87-97
  • 回收率:
    The recovery of mouse EGFL6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9794-103
    EDTA plasma (n=4)9389-96
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    60002.414 2.355 2.385 2.297
    30001.612 1.564 1.588 1.500
    15001.052 1.090 1.071 0.983
    7500.688 0.633 0.661 0.573
    3750.459 0.414 0.437 0.349
    187.50.298 0.280 0.289 0.201
    93.750.175 0.165 0.170 0.082
    00.088 0.087 0.088
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

靶点详情

  • 功能:
    May bind integrin alpha-8/beta-1 and play a role in hair follicle morphogenesis. Promotes matrix assembly.
  • 基因功能参考文献:
    1. EGLF6 mediates a paracrine mechanism of cross-talk between vascular endothelial cells and osteoblasts PMID: 21531721
    2. MAEG may play a role as a mediator regulating epithelial-mesenchymal interaction through binding to RGD-binding integrins including alpha8beta1 during hair follicle development. PMID: 15572035
  • 亚细胞定位:
    Secreted, extracellular space, extracellular matrix, basement membrane.
  • 蛋白家族:
    Nephronectin family
  • 组织特异性:
    Expressed at basement membrane of pelage follicles (at protein level).
  • 数据库链接:

    KEGG: mmu:54156

    UniGene: Mm.37707