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  4. Mouse Ectonucleotide pyrophosphatase/phosphodiesterase family member 2(ENPP2) ELISA kit

Mouse Ectonucleotide pyrophosphatase/phosphodiesterase family member 2(ENPP2) ELISA kit

  • 中文名称：
    小鼠外核苷酸焦磷酸酶/磷酸二酯酶家族成员2(ENPP2)酶联免疫试剂盒
  • 货号：
    CSB-EL007680MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse ENPP2 ELISA Kit was designed for the quantitative measurement of Mouse ENPP2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 72 ng/mL-2500 ng/mL and the sensitivity is 36 ng/mL.

  • 别名：
    Enpp2 ELISA Kit; Npps2 ELISA Kit; Pdnp2 ELISA Kit; Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 ELISA Kit; E-NPP 2 ELISA Kit; EC 3.1.4.39 ELISA Kit; Autotaxin ELISA Kit; Extracellular lysophospholipase D ELISA Kit; LysoPLD ELISA Kit
  • 缩写：
    ENPP2
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    72 ng/mL-2500 ng/mL
  • 灵敏度：
    36 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ENPP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 91-99  
    1:2 Average % 87  
    Range % 81-94  
    1:4 Average % 98  
    Range % 92-102  
    1:8 Average % 94  
    Range % 87-98  
  • 回收率：
    The recovery of mouse ENPP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 89 83-94  
    EDTA plasma (n=4) 96 91-102  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    2500 1.735 1.687 1.711 1.663  
    893 0.835 0.773 0.804 0.756  
    357.2 0.392 0.389 0.391 0.343  
    178.6 0.245 0.232 0.239 0.191  
    71.43 0.121 0.127 0.124 0.076  
    0 0.048 0.047 0.048    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development (Probable). Tumor cell motility-stimulating factor. Required for LPA production in activated platelets, cleaves the sn-1 lysophospholipids to generate sn-1 lysophosphatidic acids containing predominantly 18:2 and 20:4 fatty acids. Shows a preference for the sn-1 to the sn-2 isomer of 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF).
  • 基因功能参考文献：
    1. Data show that autotaxin (ATX)-mediated autocrine lipid signaling promotes naive pluripotency by intersecting with LIF and BMP4 signaling. PMID: 27738243
    2. This study showed that alternative autotaxin-independent pathways are likely responsible for local generation of lysophosphatidic acid in the injured lung. PMID: 27006447
    3. Hepatocyte autotaxin expression promotes liver fibrosis and liver cancer. PMID: 27981605
    4. These results indicate that ATX-lysophosphatidic acid-LPA3 signaling at the embryo-epithelial boundary induces decidualization via the canonical HB-EGF and COX-2 pathways. PMID: 28588064
    5. ATX is required for the development and maintenance of dermal fibrosis in a mouse model of bleomycin-induced systemic scleroderma (SSc) and enables 2 major mediators of SSc fibrogenesis, lysophosphatidic acid and IL-6, to amplify the production of each other. PMID: 27390295
    6. These results suggest that the post-transcriptional regulation of ATX expression by HuR and AUF1 modulates cancer cell migration. PMID: 27784781
    7. inducible, ubiquitous genetic deletion of ATX in adult mice, as well as long-term potent pharmacologic inhibition, are well tolerated, alleviating potential toxicity concerns of ATX therapeutic targeting. PMID: 26569406
    8. findings indicate that the ATX level must be carefully regulated to ensure coordinated vascular formation PMID: 25992708
    9. Autotaxin is an inflammatory mediator and therapeutic target in thyroid cancer PMID: 26037280
    10. These findings identify ATX and LPA2 as radiation-regulated genes that appear to play a physiological role in DNA repair. PMID: 26027517
    11. The results are discussed in terms of ATX regulation in wound healing and cancer.We, therefore, demonstrate the concept that accumulation of LPA in the circulation decreases ATX production PMID: 25896349
    12. blocking tumor-driven inflammation by ATX inhibition is effective in decreasing tumor growth in breast cancers where the cancer cells express negligible ATX. PMID: 26071407
    13. ATX expression and lysophosphatidic acid production are significantly enhanced in LPS treated BV-2 cells. PMID: 25053164
    14. Enpp2(+/-) mice and adipocyte-specific Enpp2 knockout mice fed a high-fat diet showed smaller weight gains and less insulin resistance than control mice, as well as more functionally active brown adipose tissue and increased energy expenditure. PMID: 24969110
    15. Autotaxin generates lysophosphatidic acid from lysophophatidylcholine. The role of this pathway in T-lymphocyte homing, inflammation, and airway remodeling is studied and inhibitors are tested. Review. PMID: 24443508
    16. Autotaxin signaling governs phenotypic heterogeneity in visceral and parietal mesothelia. PMID: 23936085
    17. We validate Enpp2/Autotaxin as one of the highest expressed signature genes in postnatal dermal papilla. PMID: 23677168
    18. We investigated the involvement of ATX in inflammatory bowel disease patients and two murine models of colitis. In the T-cell-transferred mouse model, ATX mRNA expression level gradually increased as colitis developed. PMID: 23478591
    19. Constitutive lymphocyte transmigration across the basal lamina of high endothelial venules is regulated by the autotaxin/lysophosphatidic acid axis. PMID: 23365076
    20. ATX and its enzymatic product lysophosphatidic acid influence lymphocyte migration upon and across an endothelial substratum under physiologic shear stress conditions. PMID: 22962684
    21. Data suggest that ATX is a negative regulator of brown fat adipogenesis; inhibition of ATX promotes differentiation of brown adipocytes from cultured embryonic fibroblasts. PMID: 22474126
    22. Autotaxin expression from synovial fibroblasts is essential for the pathogenesis of modeled arthritis. PMID: 22493518
    23. the ATX-LPA-LPAR axis is a critical regulator of embryonic vascular development that is conserved in vertebrates PMID: 21971049
    24. The results suggested a significant role for ATX in lung epithelial cell migration and remodelling through its ability to induce LPA production-mediated phosphorylation of PKCdelta and cortactin. PMID: 21696367
    25. Adipose-autotaxin is a negative regulator of fat mass expansion in response to an high-fat diet and contributes to plasma lysophosphatidic acid levels. PMID: 21421848
    26. The study reports the crystal structures of mouse ATX alone and in complex with lysophosphatidic acids with different acyl-chain lengths and saturations. PMID: 21240269
    27. autotaxin controls bone metastasis formation in mouse through lysophosphatidic acid-dependent activation of osteoclasts PMID: 20305819
    28. These results suggest that lysophosphatidic acid, which is converted from lysophosphatidylcholine by ATX, activates LPA1 receptors and induces dorsal root demyelination following nerve injury, which causes neuropathic pain. PMID: 21062487
    29. These results reveal the signal transduction defects that underlie the abnormalities in Enpp2(-/-) embryos. PMID: 20692235
    30. autotaxin has a role in the development of adipose tissue and obesity-associated pathologies PMID: 12642576
    31. Up-regulation of adipocyte autotaxin expression, which could be related to the severe type 2 diabetes phenotype and adipocyte insulin resistance. PMID: 15700135
    32. Furthermore, the effect of ATX in preventing apoptosis appears to be mediated through the G-protein-coupled receptor pathway followed by the activation of phosphoinositide 3-kinase and Akt pathway leading to enhanced cell survival. PMID: 16219296
    33. These results reveal a critical role for ATX in vascular development, indicate that ATX is the major LPA-producing enzyme in vivo, and suggest that the vascular defects in ATX-deficient embryos may be explained by loss of LPA signaling through Galpha13. PMID: 16782887
    34. ATX has a role in stabilizing vessels through novel LPA signaling pathways PMID: 16829511
    35. Non-conserved roles for ATX during neural development and organogenesis. PMID: 17366625
    36. ATX deletion is lethal at an early stage of the development PMID: 17628547
    37. In vivo, an extracellular lysophospholipase D such as murine autotaxin may participate in leiomyoma growth through local lysophosphatidic acid formation. PMID: 18024704
    38. Murine and human autotaxin alpha, beta, and gamma isoforms: gene organization, tissue distribution, and biochemical characterization. PMID: 18175805
    39. These findings suggest that lysophosphatidic acid (LPA) biosynthesis through ATX is the source of LPA for LPA1 receptor-mediated neuropathic pain. PMID: 18261210
    40. Facilitates the entry of lymphocytes from the blood into secondary lymphoid organs. PMID: 18327261
    41. Functional ATX is selectively expressed in high endothelial venules (HEVs) of both lymph nodes and Peyer's patches. PMID: 18818380
    42. LPA production by autotaxin/lysoPLD regulates murine hemostasis and thrombosis and suggest that binding of autotaxin/lysoPLD to activated platelets may provide a mechanism to localize LPA production. PMID: 19139100
    43. Enpp2 is a candidate gene controlling lung function development in mice. PMID: 17804602

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  • 相关疾病：
    May contribute to obesity (PubMed:15700135).
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Nucleotide pyrophosphatase/phosphodiesterase family
  • 组织特异性：
    Expressed in brain and adipose tissue.
  • 数据库链接：

    KEGG: mmu:18606

    UniGene: Mm.250256