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  4. Mouse Disintegrin and metalloproteinase domain-containing protein 33(ADAM33) ELISA kit

Mouse Disintegrin and metalloproteinase domain-containing protein 33(ADAM33) ELISA kit

  • 中文名称：
    小鼠解整合素样金属蛋白酶33(ADAM33)酶联免疫试剂盒
  • 货号：
    CSB-EL001290MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse ADAM33 ELISA Kit was designed for the quantitative measurement of Mouse ADAM33 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 别名：
    Adam33Disintegrin and metalloproteinase domain-containing protein 33 ELISA kit; ADAM 33 ELISA kit; EC 3.4.24.- ELISA kit
  • 缩写：
    ADAM33
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADAM33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %93
    Range %88-97
    1:2Average %84
    Range %80-88
    1:4Average %95
    Range %90-100
    1:8Average %104
    Range %99-109
  • 回收率：
    The recovery of mouse ADAM33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9085-94
    EDTA plasma (n=4)9287-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.658 2.552 2.605 2.434
    5001.881 2.006 1.944 1.773
    2501.128 1.204 1.166 0.995
    1250.627 0.653 0.640 0.469
    62.50.372 0.401 0.387 0.216
    31.20.276 0.264 0.270 0.099
    15.60.234 0.239 0.237 0.066
    00.174 0.167 0.171
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 基因功能参考文献：
    1. The mouse and human predicted proteins consisted of 797 and 813 amino acids, respectively, and they shared 70% homology of the entire amino acid sequence. PMID: 11814695
    2. Identification and preliminary characterization of mouse Adam33. PMID: 11897009
    3. ADAM33 is not essential for growth or reproduction in the mouse and does not modulate baseline or allergen-induced airway responsiveness. PMID: 16943435
    4. Adam33/Adam33 shows 2 significant increments in expression during lung morphogenesis, suggesting important developmental regulation. PMID: 19665773
  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 数据库链接：