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  4. Mouse D-Lactate Dehydrogenase,D-LDH ELISA Kit

Mouse D-Lactate Dehydrogenase,D-LDH ELISA Kit

  • 中文名称：
    小鼠D-乳酸脱氢酶(D-LDH)酶联免疫试剂盒
  • 货号：
    CSB-E11723m
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse D-LDH ELISA Kit was designed for the quantitative measurement of Mouse D-LDH protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 4.69 mU/mL-300 mU/mL and the sensitivity is 1.17 mU/mL.

  • 别名：
    Ldhd ELISA Kit; Probable D-lactate dehydrogenase ELISA Kit; mitochondrial ELISA Kit; DLD ELISA Kit; Lactate dehydrogenase D ELISA Kit; EC 1.1.2.4 ELISA Kit
  • 缩写：
    LDHD
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    4.69 mU/mL-300 mU/mL
  • 灵敏度：
    1.17 mU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse D-LDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 85  
    Range % 81-89  
    1:2 Average % 93  
    Range % 90-96  
    1:4 Average % 87  
    Range % 83-90  
    1:8 Average % 98  
    Range % 94-102  
  • 回收率：
    The recovery of mouse D-LDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 85-95  
    EDTA plasma (n=4) 104 101-107  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/ml OD1 OD2 Average Corrected  
    300 2.278 2.304 2.291 2.111  
    150 1.422 1.500 1.461 1.281  
    75 0.932 0.921 0.927 0.747  
    37.5 0.623 0.624 0.624 0.444  
    18.75 0.385 0.394 0.390 0.210  
    9.38 0.287 0.275 0.281 0.101  
    4.69 0.242 0.231 0.237 0.057  
    0 0.181 0.179 0.180    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Involved in D-lactate, but not L-lactate catabolic process.
  • 基因功能参考文献：
    1. identification and characterization of human and mouse D-lactate dehydrogenase (NAD) [D-LDH] PMID: 12127981
  • 亚细胞定位：
    Mitochondrion.
  • 蛋白家族：
    FAD-binding oxidoreductase/transferase type 4 family
  • 组织特异性：
    Readily detected in liver and kidney, with a weaker signal observed in heart, skeletal muscle, stomach, brain, and lung.
  • 数据库链接：