Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Mouse Corticotropin-releasing factor-binding protein(CRHBP) ELISA kit

Mouse Corticotropin-releasing factor-binding protein(CRHBP) ELISA kit

  • 中文名称：
    小鼠促肾上腺皮质激素释放因子结合蛋白(CRHBP)酶联免疫试剂盒
  • 货号：
    CSB-EL005964MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse CRHBP ELISA Kit was designed for the quantitative measurement of Mouse CRHBP protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 别名：
    CrhbpCorticotropin-releasing factor-binding protein ELISA kit; CRF-BP ELISA kit; CRF-binding protein ELISA kit; Corticotropin-releasing hormone-binding protein ELISA kit; CRH-BP ELISA kit
  • 缩写：
    CRHBP
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CRHBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 89-97  
    1:2 Average % 96  
    Range % 91-103  
    1:4 Average % 97  
    Range % 94-100  
    1:8 Average % 93  
    Range % 88-98  
  • 回收率：
    The recovery of mouse CRHBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 93-100  
    EDTA plasma (n=4) 91 84-98  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.582 2.776 2.679 2.501  
    500 2.193 2.223 2.208 2.030  
    250 1.446 1.465 1.456 1.278  
    125 0.821 0.865 0.843 0.665  
    62.5 0.565 0.612 0.589 0.411  
    31.25 0.319 0.295 0.307 0.129  
    15.6 0.256 0.246 0.251 0.073  
    0 0.178 0.177 0.178 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Binds CRF and inactivates it. May prevent inappropriate pituitary-adrenal stimulation in pregnancy.
  • 基因功能参考文献：
    1. Repeated cycles of binge drinking alter CRF-BP mRNA expression in the medial prefrontal cortex. PMID: 27374820
    2. Ventral tegmental area CRH-BP is involved in the initial stages of escalated ethanol drinking. PMID: 26247973
    3. These studies demonstrate a role for the pituitary CRH-BP in attenuating the HPA response to stress in female mice. PMID: 25731977
    4. estrogen-regulated expression of corticotropin-releasing hormone-binding protein in lactotropes in female mice suggests that the pituitary is an important site for interactions between the hypothalamic-pituitary-adrenal axis and other endocrine systems PMID: 12446601
    5. Evidence is presented suggesting that the CRH-BP may be required for corticotropin-releasing factor-induced potentiation of NMDA receptors in dopaminergic neurons of the ventral tegmental area. PMID: 12895416
    6. GnRH induces a 3.7-fold increase in CRH-BP mRNA levels via multiple intracellular signaling pathways and a multipartite GnRH response element. PMID: 15976007
    7. analysis of regulation and function of CRH-BP [review] PMID: 16368564
    8. Deletion of corticotropin-releasing factor binding protein selectively impairs maternal, but not intermale aggression. PMID: 18929624

    显示更多

    收起更多

  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    CRF-binding protein family
  • 数据库链接：