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Mouse Corticosterone,CORT ELISA Kit

  • 中文名称:
    小鼠皮质酮/肾上腺酮(CORT)酶联免疫试剂盒
  • 货号:
    CSB-E07969m
  • 规格:
    96T/48T
  • 价格:
    ¥3800/¥2500
  • 其他:

产品详情

  • 产品描述:
        CORT(Corticosterone)是一种糖皮质激素,主要由肾上腺分泌,是压力激素的代表,能够帮助机体适应各种应激情况。在应激状态下,CORT能够抑制免疫系统、促进葡萄糖生成等生理反应。
        j9九游会登录入口首页生物所提供的Mouse Corticosterone,CORT ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测鼠血清、血浆、组织匀浆样本中的CORT,其灵敏度为3.9 ng/ml,检测范围为15.6 ng/ml-1000 ng/ml。
  • 别名:
    N/A
  • 缩写:
    CORT
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    15.6 ng/ml-1000 ng/ml.
  • 灵敏度:
    3.9 ng/ml.
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 本试剂盒所含材料:
      • A micro ELISA plate --- The 96-well plate has been pre-coated with goat anti-rabbit CORT antibody.
      • Six vials lyophilized standard (0.5 ml/bottle) --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • Anti-mouse CORT Antibody (1 x 6 ml) --- Act as the detection antibody.
      • HRP-conjugate (1 x 6 ml) --- HRP catalyzes TMB substrate to elicit a chromogenic reaction.
      • Wash Buffer (20 x concentrate) (1 x 15 ml) --- Wash away unbound or free substances.
      • Substrate A (1 x 7 ml) --- Mix with substrate B, and the TMB is catalyzed by HRP to elicit a chromogenic signal.
      • Substrate B (1 x 7 ml) --- Mix with substrate A, and the TMB is catalyzed by HRP to elicit a chromogenic signal.
      • Stop Solution 1 x 7 ml --- Stop the color development, and the eventual solution color turns yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microwell plate when incubation.
      • An Instruction manual

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  • 本试剂盒不含材料:
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm to 630 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理:
  • 货期:
    3-5 working days

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靶点详情

  • 最新研究进展:
    CORT是一种主要由肾上腺皮质合成的类固醇激素,具有重要的生理功能,例如调节葡萄糖代谢、免疫系统和心血管系统等。最新研究表明,CORT在多种疾病的发生和发展中也发挥着重要作用。例如,CORT与焦虑和抑郁等心理疾病的发病机制有关。此外,一些研究还发现CORT在自身免疫性疾病、肿瘤、神经系统疾病和心血管疾病等方面发挥着重要作用。