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  4. Mouse Complement fragment 4a,C4a ELISA Kit

Mouse Complement fragment 4a,C4a ELISA Kit

  • 中文名称：
    小鼠过敏毒素/补体片断4a(C4a)酶联免疫试剂盒
  • 货号：
    CSB-E08517m
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse C4a ELISA Kit was designed for the quantitative measurement of Mouse C4a protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 39 pg/mL-2500 pg/mL and the sensitivity is 9.75 pg/mL.

  • 缩写：
    C4a
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    39 pg/mL-2500 pg/mL
  • 灵敏度：
    9.75 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse C4a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1000 Average % 96  
    Range % 87-105  
    1:2000 Average % 90  
    Range % 84-100  
    1:4000 Average % 95  
    Range % 82-103  
    1:8000 Average % 96  
    Range % 84-103  
  • 回收率：
    The recovery of mouse C4a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 87-105  
    EDTA plasma (n=4) 96 82-101  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2500 2.786 2.687 2.737 2.599  
    1250 2.136 2.042 2.089 1.951  
    625 1.253 1.265 1.259 1.121  
    312 0.784 0.752 0.768 0.630  
    156 0.543 0.521 0.532 0.394  
    78 0.364 0.342 0.353 0.215  
    39 0.242 0.239 0.241 0.103  
    0 0.136 0.140 0.138    
  • 数据处理：
  • 货期：
    3-5 working days

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