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  4. Mouse Complement 4,C4 ELISA Kit

Mouse Complement 4,C4 ELISA Kit

  • 中文名称：
    小鼠补体蛋白4(C4)酶联免疫试剂盒
  • 货号：
    CSB-E08707m
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse C4B ELISA Kit was designed for the quantitative measurement of Mouse C4B protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 μg/mL-10 μg/mL and the sensitivity is 0.039 μg/mL.

  • 别名：
    C4b ELISA kit; C4Complement C4-B [Cleaved into: Complement C4 beta chain; Complement C4 alpha chain; C4a anaphylatoxin; Complement C4 gamma chain] ELISA kit
  • 缩写：
    C4B
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 μg/mL-10 μg/mL
  • 灵敏度：
    0.039 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse C4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:100Average %91
    Range %86-95
    1:200Average %102
    Range %97-107
    1:400Average %91
    Range %85-97
    1:800Average %97
    Range %91-103
  • 回收率：
    The recovery of mouse C4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-98
    EDTA plasma (n=4)9790-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/mOD1OD2AverageCorrected
    102.605 2.648 2.627 2.463
    52.312 2.335 2.324 2.160
    2.51.857 1.867 1.862 1.698
    1.251.136 1.103 1.120 0.956
    0.6250.798 0.785 0.792 0.628
    0.3120.435 0.412 0.424 0.260
    0.1560.316 0.304 0.310 0.146
    00.163 0.165 0.164
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. Catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens.
  • 基因功能参考文献：
    1. These data show that Borrelia burgdorferi OspC inhibits the classical and lectin complement pathways and competes with complement protein C2 for C4b binding. PMID: 28873507
    2. C4 mediated synapse elimination during postnatal development PMID: 26814963
    3. role in suppressing autoantibody production elicited by S. pneumoniae antigens PMID: 25339671
    4. a deficiency in C4b does not alter radiation-induced lung disease in the C57BL/6 mouse model PMID: 23259761
    5. C4 modulates interstitial inflammation in experimental glomerulonephritis. PMID: 11726230
    6. Complement C4 provides an important protective role against the development of systemic lupus erythematosus. PMID: 11801636
    7. identified the murine follicular dendritic cell marker, FDC-M2, as complement c4. FDC-M2 was detectable at sites of immune complex-mediated inflammatory disease PMID: 12115608
    8. C4 normally helps prevent early stages of autoimmune disease and that C4 deficiency predisposes to abnormal regulation of autoreactive B cells. PMID: 12207352
    9. Macrophages derived from bone marrow produce sufficient C4 protein to restore the humoral response to NP-KLH hapten in C4-deficient animals when administered along with thymus-dependent antigen. PMID: 12421924
    10. effects of complement depletion and deficiencies of C4, C5, and complement receptors 1 and 2 on mouse survival after intravenous exposure to S aureus PMID: 15192652
    11. We found that deletion of the C4 gene does not significantly change either the time of onset or the severity and tempo of myelin oligodendrocyte-induced EAE compared with controls with a fully intact complement system. PMID: 15390104
    12. In comparison with C57BL/6 mice, MRL/1pr mice had depressed C4 levels as early as 3 weeks of age. PMID: 17971229
    13. The increased mortality of C4(-/-) or Factor B(-/-) mice indicates that these two pathways of complement activation are required for survival in mousepox. PMID: 19112490
    14. Increased transcription of the complement C4 gene in the Rag1(-/-) intestine. PMID: 19200601

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  • 亚细胞定位：
    Secreted. Cell junction, synapse. Cell projection, axon. Cell projection, dendrite.
  • 数据库链接：