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  4. Mouse Collagen alpha-1(V) chain (COL5A1) ELISA kit

Mouse Collagen alpha-1(V) chain (COL5A1) ELISA kit

  • 中文名称：
    小鼠V型胶原α1链(COL5A1)酶联免疫试剂盒
  • 货号：
    CSB-EL005748MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 别名：
    Col5a1Collagen alpha-1(V) chain ELISA kit
  • 缩写：
    COL5A1
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/ml -20 ng/ml
  • 灵敏度：
    0.078 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Per customers request, please provide the following information regarding Mouse Collagen alpha-1 (V) chain, COL5A1 ELISA Kit CSB-EL005748MO
1. Please provide current manual
2. If competitive please provide following information:
a. What is the standard?
b. What is the immunogen this kit detects? Sequence Range?
c. What is the antibody in this kit?
3. If Sandwich please provide the following information:
a. What is the standard? (Recombinant full-length protein? Partial protein (range?)?, peptide (sequence range?), native protein?)
b. If recombinant protein, then what is the host?
c. Is the capture antibody a monoclonal or polyclonal?
d. What is the host of the capture antibody?
e. What is the immunogen of the capture antibody?
f. Is the detection antibody a monoclonal or polyclonal?
g. What is the host of the detection antibody?
h. What is the immunogen of the detection antibody?

A:
Thanks for your inquiry!
CSB-EL005748MO
Pls find attached manual.
This kit is based on double antibody sandwich method. Pls refer to: https://www.uniprot.org/uniprot/O88207.
Capture antibody is rabbit polyclonal antibody and detection antibody is rabbit polyclonal antibody.
Immunogen is 293 cell-derived recombinant protein.
Standard is 293 cell-derived recombinant protein.

靶点详情

  • 功能：
    Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. Transcriptionally activated by CEBPZ, which recognizes a CCAAT-like motif, CAAAT in the COL5A1 promoter.
  • 基因功能参考文献：
    1. COL5A1 may contribute the metastasis of lung adenocarcinoma. PMID: 29702185
    2. Significantly decreased fibroblast proliferation was observed in Col5a1(+/-) compared to Col5a1(+/+) fibroblasts. PMID: 26713685
    3. Col5a1 knockout stroma demonstrated severe dysfunctional regulation of fibrillogenesis. PMID: 22159420
    4. Sp7/Osterix was observed to participate in the activation of the Col5a1 promoter during osteoblast differentiation. PMID: 20888414
    5. Col(V) is an established autoantigen in atherosclerotic mice, and Col(V) autoimmunity is a consistent feature, with variable T helper type (Th)1 cell pathway involvement. PMID: 20814021
    6. analysis of processing of the Pro-alpha1(V)Pro-alpha2(V)Pro-alpha3(V) procollagen heterotrimer PMID: 15136578
    7. CBF activated the Col5a1 promoter suggesting that CBF is a key factor in the coordinated expression of type I and V collagen genes PMID: 15246108
    8. collagen type V has a critical role in early fibril initiation PMID: 15383546
    9. COL5A1 is regulated by TGF-beta in osteoblasts PMID: 15579311
    10. col5a1 haploinsufficiency disrupts collagen fibril assembly at multiple stages PMID: 16492673
    11. Type V collagen contributes to the development of functional connective tissues during embryogenesis. PMID: 17024418
    12. ColVa1+/- and ColXIa1-/- mutants were used to identify roles for ColVa1 and ColXIa1 in ventricular myocardial morphogenesis and heart valve development. PMID: 17029294

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Fibrillar collagen family
  • 组织特异性：
    Widely expressed. Isoform 2 is more highly expressed in liver, kidney and lung.
  • 数据库链接：

    KEGG: mmu:12831

    STRING: 10090.ENSMUSP00000028280

    UniGene: Mm.7281