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  4. Mouse Chordin-like protein 1(CHRDL1) ELISA kit

Mouse Chordin-like protein 1(CHRDL1) ELISA kit

  • 中文名称：
    小鼠腱蛋白样蛋白1(CHRDL1)酶联免疫试剂盒
  • 货号：
    CSB-EL005378MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse CHRDL1 ELISA Kit was designed for the quantitative measurement of Mouse CHRDL1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 1.95 pg/mL .

  • 别名：
    Chrdl1 ELISA kit; Ng1 ELISA kit; Nrln1Chordin-like protein 1 ELISA kit; Neuralin-1 ELISA kit; Neurogenesin-1 ELISA kit; Ventroptin ELISA kit
  • 缩写：
    CHRDL1
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    1.95 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CHRDL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %88-95
    1:2Average %101
    Range %97-106
    1:4Average %98
    Range %92-103
    1:8Average %86
    Range %82-89
  • 回收率：
    The recovery of mouse CHRDL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-102
    EDTA plasma (n=4)8985-95
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.377 2.356 2.367 2.217
    5001.599 1.687 1.643 1.493
    2500.933 0.997 0.965 0.815
    1250.541 0.568 0.555 0.405
    62.50.355 0.326 0.341 0.191
    31.20.222 0.236 0.229 0.079
    7.80.154 0.167 0.161 0.011
    00.144 0.156 0.150 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Seems to antagonize the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation. Antagonizes the function of BMP7 and may thus play an important role in the embryonic bone formation. Shows no inhibitory effect on the inducing activity of BMP2. Plays a role during anterior segment eye development.
  • 基因功能参考文献：
    1. CHRDL-1 is a new regulatory target of miR-134 during cortical neuronal differentiation; this is a novel role for miR-134 in the modulation of cortical development. PMID: 21228099
    2. Reperfusion injury of proximal kidney tubules causes a reduction of Chrdl1 expression. PMID: 19357253
  • 亚细胞定位：
    Secreted.
  • 组织特异性：
    Expressed in heart, brain, lung, liver, kidney and testis.
  • 数据库链接：