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  4. Mouse Bone marrow proteoglycan(PRG2) ELISA kit

Mouse Bone marrow proteoglycan(PRG2) ELISA kit

  • 中文名称：
    小鼠骨髓蛋白多糖(PRG2)酶联免疫试剂盒
  • 货号：
    CSB-EL018670MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse PRG2 ELISA Kit was designed for the quantitative measurement of Mouse PRG2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.9 ng/mL-250 ng/mL and the sensitivity is 0.98 ng/mL.

  • 别名：
    Prg2 ELISA Kit; Mbp-1 ELISA Kit; Bone marrow proteoglycan ELISA Kit; BMPG ELISA Kit; Proteoglycan 2) [Cleaved into: Eosinophil granule major basic protein ELISA Kit; EMBP ELISA Kit; MBP)] ELISA Kit
  • 缩写：
    PRG2
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.9 ng/mL-250 ng/mL
  • 灵敏度：
    0.98 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PRG2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:5Average %94
    Range %89-97
    1:10Average %103
    Range %98-107
    1:20Average %90
    Range %84-94
    1:40Average %98
    Range %92-102
  • 回收率：
    The recovery of mouse PRG2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9185-96
    EDTA plasma (n=4)9488-97
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    2502.772 2.664 2.718 2.597
    1252.026 2.076 2.051 1.930
    62.51.218 1.200 1.209 1.088
    31.250.677 0.654 0.666 0.545
    15.60.392 0.387 0.390 0.269
    7.80.299 0.287 0.293 0.172
    3.90.208 0.203 0.206 0.085
    00.122 0.120 0.121
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Cytotoxin and helminthotoxin. MBP also induces non-cytolytic histamine release from basophils. It is involved in antiparasitic defense mechanisms and immune hypersensitivity reactions.
  • 基因功能参考文献：
    1. data show that bioactive phospholipids and PRG-2 are critical for guiding thalamic axons to their proper cortical targets. PMID: 27641493
    2. The absence of MBP-1 and EPX promoted a concomitant loss of eosinophil lineage-committed progenitors in the marrow, identifying a specific blockade in eosinophilopoiesis as the causative event. PMID: 23736699
    3. major basic protein decreases epithelial barrier function and in this manner contributes to the pathogenesis of inflammatory bowel diseases. PMID: 16227527
    4. Host protection against Brugia pahangi infections was unimpaired in mice deficient in major basic protein 1. PMID: 16299347
    5. Mice deficient for either eosinophil peroxidase or major basic protein on the 129/SvJ background developed significantly higher worm burdens than wild-type mice.(major basic protein 1) PMID: 16926417
    6. Eosinophil-derived MBP-1 plays a significant role in regulating muscular dystrophy by attenuating the cellular immune response and promoting tissue fibrosis that can eventually contribute to increased mortality. PMID: 18430716

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  • 亚细胞定位：
    Cytoplasmic granule.
  • 数据库链接：