Mouse Beta-type platelet-derived growth factor receptor(PDGFRB) ELISA kit

Tyrosine-protein kinase that acts as cell-surface receptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB, and plays an essential role in the regulation of embryonic development, cell proliferation, survival, differentiation, chemotaxis and migration. Plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. Required for normal development of the cardiovascular system. Required for normal recruitment of pericytes (mesangial cells) in the kidney glomerulus, and for normal formation of a branched network of capillaries in kidney glomeruli. Promotes rearrangement of the actin cytoskeleton and the formation of membrane ruffles. Binding of its cognate ligands - homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFD -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PLCG1, PIK3R1, PTPN11, RASA1/GAP, CBL, SHC1 and NCK1. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to the activation of the AKT1 signaling pathway. Phosphorylation of SHC1, or of the C-terminus of PTPN11, creates a binding site for GRB2, resulting in the activation of HRAS, RAF1 and down-stream MAP kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation and activation of SRC family kinases. Promotes phosphorylation of PDCD6IP/ALIX and STAM. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.

1. PDGFRbeta was activated around the injury site after experimental traumatic brain injury (TBI), and primarily expressed in astrocytes, microglia, OPC and leukocytes in the boundary of the lesion site, suggesting PDGFRbeta was involved in glial scar formation. Then the PDGFR inhibitor (AG1296) was administered following TBI. Reactive astrocytes were significantly inhibited in AG1296-treated mice. PMID: 28684344
2. Increasing the adipogenic capacity of Pdgfrbeta(+) precursors through Pparg overexpression results in healthy visceral white adipose tissue expansion in obesity and adiponectin-dependent improvements in glucose homeostasis. PMID: 29497032
3. The data of this study revealed an acute accumulation of PDGFRbeta+ BBB-related cells in degenerating brain areas, which can be long lasting, suggesting an active role for PDGFRbeta-signaling in blood vessel and post-injury tissue recovery. PMID: 27778377
4. Beta platelet-derived growth factor is induced in hepatic stellate cells (HSCs) following surgical partial hepatectomy, and its deletion in HSCs leads to prolonged liver injury in mice. However, there is no significant difference in liver regeneration. PMID: 26256287
5. The results provide functional evidence that elevated PDGFRbeta signaling causes tissue wasting or overgrowth reminiscent of human genetic syndromes and that the STAT1 pathway is a crucial modulator of this phenotypic spectrum. PMID: 28924035
6. Identify PDGFRbeta as a driver in activating Akt/mTORC1 nexus for high glucose-mediated expression of collagen I (alpha2) in proximal tubular epithelial cells, which contributes to tubulointerstitial fibrosis in diabetic nephropathy. PMID: 28424212
7. Data show that three platelet-derived growth factor receptor beta (PDGFRB) mutants (R561C, P660T and N666K) were able to transform NIH3T3 and Ba/F3 cells to different extents. PMID: 26455322
8. data therefore collectively suggest that upon TGFbeta stimulation, SP1 recruits SMAD2 to the promoter of Pdgfrb to up-regulate its expression and thus Pdgfrb is a direct downstream target of the TGFbeta/SMAD2 signaling PMID: 28426709
9. PdgfrbetaF7/F7 mice between 4-6 and 36-48 weeks of age developed a region-dependent loss in pericyte coverage (22-46, 24-44 and 4-31%) and cell numbers (36-49, 34-64 and 11-36%), reduction in capillary length (20-39, 13-46 and 1-30%), and an increase in extravascular fibrinogen-derived deposits (3.4-5.2, 2.8-4.1 and 0-3.6-fold) demonstrating BBB breakdown in the cortex, hippocampus and thalamus, respectively. PMID: 28441414
10. there is great possibility that EPCs overexpressing PDGFR-beta enhanc VSMC apoptosis and suppress VSMC migration by competitive consumption of PDGF-BB in the early phase after carotid artery injury in mice. PMID: 27619504
11. Data show that oligodendrocyte transcription factor 2 (Olig2) deletion causes platelet-derived growth factor receptor (PDGFR) downregulation and reciprocal epidermal growth factor receptor (EGFR) upregulation. PMID: 27165742
12. Decreased expression of PDGFR-beta in vascular smooth muscle cells was associated with thrombosis in vivo. PMID: 27198239
13. Our results highlight the need for careful interpretation of lineage tracing using constitutive Cre lines that cannot discriminate active from historical expression. The early vascular targeting by the Pdgfrb-Cre also warrants consideration for its use in studies of mural cells PMID: 27060598
14. Pdgfrbeta(+) cells do not significantly contribute to the initial cold-induced recruitment of beige adipocytes in WAT; it is only after prolonged cold exposure that these cells differentiate into beige adipocytes. PMID: 26626462
15. Results suggested that increased bFGF upregulates the expression of PDGFRbeta and may enhance PDGFRbeta-mediated pericyte functions after brain ischemia PMID: 26569132
16. The role of the S1PR3/PDGFR-beta/Akt pathway in sphingosine-1-phosphate -induced endothelial progenitor cells migration and angiogenesis PMID: 26208383
17. Increased PDGFRbeta signalling promotes advanced plaque formation at novel sites in the thoracic aorta and coronary arteries. PMID: 26183159
18. This study demonstrated that PDGFRbeta-expressing cells appear to arise from vascular wall pericytes in peri-infarct areas and then extend toward the ischemic core while producing ECM proteins, fibronectin and collagen type I, after MCAO. PMID: 25510317
19. These results suggest that gonocyte and F9 cell differentiation is regulated via cross talk between retinoic acid and PDGFRs using different downstream pathways. PMID: 25380237
20. The study shows that PDGFRB and FGFR2 mediate endothelial cell differentiation capability of triple negative breast carcinoma. PMID: 24747080
21. Radial glia require PDGFD-PDGFRbeta signalling in human but not mouse neocortex PMID: 25391964
22. The study demonstrates changes in pericyte expression of NG2 and PDGFRB and vascular permeability in the sensory circumventricular organs by chronic osmotic stimulation. PMID: 23629811
23. the expression and function of PDGF-Rbeta in lower esophageal sphincter (LES)circular smooth muscle (CSM) from W/W(v) mice is impaired, providing further evidence that LES CSM is abnormal in W/W(v) mutants. PMID: 24383871
24. These results are the first to identify EphB4 and its cross-talk with PDGFRbeta as unexpected vital determinants of tumor cell survival in alveolar rhabdomyosarcoma. PMID: 24733895
25. ephrin-B2 is an important regulator of PDGFRbeta endocytosis and thereby acts as a molecular switch controlling the downstream signaling activity of this receptor PMID: 24298057
26. Suggest that myocardin regulates vascular injury response via miR-24/miR-29a/PDGFRB pathway. PMID: 23825366
27. Selective activation of oxidized PTP1B by the thioredoxin system modulates PDGF-beta receptor tyrosine kinase signaling. PMID: 23901112
28. These results suggest that PDGFR-beta signaling is important for the self-renewal and multipotency of neural stem cells. PMID: 23454370
29. These results suggest that PDGFRB may contribute to the aggressive phenotype of colorectal tumors with mesenchymal properties, most likely downstream of platelet activation and TGFB signaling. PMID: 23441134
30. The Pdgfrb(redeye/redeye) mice exhibit all of the features of nonproliferative diabetic retinopathy, including retinal neurodegeneration. PMID: 23633653
31. The H2O2-induced src/PDGFRbeta/SK1 signaling cascade was impaired in nSMase2-deficient fro/fro cells and was rescued by exogenous C2Cer that activated src/PDGFRbeta/SK1. PMID: 23651497
32. determined that PDGF-regulated glycolysis occurs independent of PDGF-regulated proliferation but requires the activation of AKT, a known metabolic regulator in tumor PMID: 23322009
33. cell proliferation induced by Wnt3a in osteoblastic cells is mediated by a dishevelled-dependent and beta-catenin-independent pathway, which involves the transactivation of PDGF receptors. PMID: 22927028
34. Impairment in hippocampal long-term potentiation and in hippocampus-dependent memory formation are seen in PDGFR-beta knockout mice. PMID: 21997856
35. findings demonstrate a novel capacity for p19(Arf) to control Pdgfrbeta expression by p53-dependent and -independent mechanisms involving RNA transcription and protein synthesis, respectively, to promote the vascular remodeling needed for normal vision PMID: 22907756
36. Data indicate that the proteolytic processing of full-length PDGF-D is required for PDGF-D activation of preosteoclasts, and that beta-PDGFR is present in activated osteoclasts. PMID: 22158043
37. Blocking the PDGFRbeta pathway with AG1295 markedly promotes osteoblast differentiation and matrix mineralization. PMID: 22890161
38. beta-catenin transcriptional activity directly regulated endothelial expression of platelet-derived growth factor B, leading to mural cell recruitment thereby contributing to vascular quiescence PMID: 22908324
39. a) PDGFR-beta could be used as a marker for TCs and b) TCs are presumably a transitional population in the complex process of mural cell recruitment during angiogenesis and vascular remodelling PMID: 22188481
40. the involvement of CCL5 in the pathogenesis of colorectal carcinoma PMID: 22205974
41. From 3 to 28 days after middle cerebral artery occlusion, postnatally induced systemic PDGFR-beta knockout mice (Esr-KO) exhibited the delayed recovery of body weight and behavior, and larger infarction volume than controls. PMID: 21952111
42. Data indicate that signalling through both PDGFR-beta and CaMKII-alpha contributes to progression of diabetic nephropathy, with increase in oxidative stress and mesangial expansion. PMID: 21833587
43. Data show a dramatic discrepancy between ROSA26 reporter activity and Pdgfrb promoter driven Cre dependent myc-tagged Cthrc1 transgene expression. PMID: 21557454
44. Platelet-derived growth factor (PDGF)-BB produces NO-mediated relaxation and PDGF receptor beta-dependent tonic contraction in murine iliac lymph vessels. PMID: 21535294
45. PDGFR-beta plays a role in the structure and function of kidney glomeruli in the mouse PMID: 20693161
46. PDGFR-beta plays an important role in cognitive and socioemotional functions. PMID: 21437241
47. A novel signaling pathway that links ATM via CREB to the transcription factor ZFHX3, which in turn promotes survival of neurons by inducing expression of platelet-derived growth factor receptor beta, is reported. PMID: 20876357
48. Myocardin may contribute to the differentiation of CXCR4(+)/PDGFRbeta(+) progenitor cells into smooth muscle cells induced by hypoxia, which leads to the muscularization of alveolar arterioles. PMID: 20484220
49. Data show that in high-fat-fed apolipoprotein E(-/-) mice, inhibition of PDGFRbeta tyrosine kinase activity leads to inhibition of GAG synthesis on vascular PGs and aortic lipid area. PMID: 20610572
50. PDGFRbeta activates ADAM17 and promotes metalloproteinase-dependent cross-talk between the PDGFRbeta and epidermal growth factor receptor (EGFR) signaling pathways PMID: 20529858

显示更多

收起更多

Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle. Lysosome lumen. Note=After ligand binding, the autophosphorylated receptor is ubiquitinated and internalized, leading to its degradation.

Protein kinase superfamily, Tyr protein kinase family, CSF-1/PDGF receptor subfamily

Weakly expressed in glomerular mesangial cells and interstitial cells. Up-regulated in areas of renal fibrosis. In mice with unilateral ureteral obstruction, increased expression in interstitial cells at day 4 and expression is markedly elevated at day 7

KEGG: mmu:18596

STRING: 10090.ENSMUSP00000025522

UniGene: Mm.4146