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  4. Mouse Apolipoprotein M(APOM) ELISA kit

Mouse Apolipoprotein M(APOM) ELISA kit

  • 中文名称：
    小鼠载脂蛋白M(APOM)酶联免疫试剂盒
  • 货号：
    CSB-EL001947MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse APOM ELISA Kit was designed for the quantitative measurement of Mouse APOM protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

  • 别名：
    Apom ELISA kit; Ng20Apolipoprotein M ELISA kit; Apo-M ELISA kit; ApoM ELISA kit
  • 缩写：
    APOM
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.56 ng/mL-100 ng/mL
  • 灵敏度：
    0.39 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse APOM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1000Average %86
    Range %81-92
    1:2000Average %95
    Range %88-99
    1:4000Average %93
    Range %86-98
    1:8000Average %100
    Range %96-107
  • 回收率：
    The recovery of mouse APOM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9488-99
    EDTA plasma (n=4)9994-106
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    1002.503 2.569 2.536 2.369
    502.278 2.207 2.243 2.076
    251.845 1.864 1.855 1.688
    12.51.317 1.329 1.323 1.156
    6.250.861 0.899 0.880 0.713
    3.120.582 0.592 0.587 0.420
    1.560.412 0.426 0.419 0.252
    00.168 0.166 0.167 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.
  • 基因功能参考文献：
    1. apoM-S1P-S1PR1 signaling might underlie the pathogenesis of ALI and apoM could have physiological benefits to alleviate LPS-induced ALI. PMID: 29260347
    2. These S1P-induced enhancements in growth factors and chemotactic cytokines in retinal pigment epithelium cells were significantly inhibited by ApoM treatment. Additionally, in vivo experiments using a laser-induced choroidal neovascularization (CNV) murine model demonstrated that intravitreal ApoM injection significantly reduced the progression of CNV formation. PMID: 29301231
    3. This study highlights the complexity of Apom/S1P in atherosclerosis and challenges the notion that the Apom/S1P complex is anti-atherogenic, at least in Apoe-deficient mice. PMID: 28866363
    4. This suggests that the autophagy dysfunction caused by the deficiency of ApoM is an important factor in hepatic steatosis (triglyceride accumulation). ApoM plays a key role in normal autophagy activity in the liver and thereby further regulates the metabolism of liver lipids, particularly triglycerides. PMID: 29288662
    5. HDL facilitates S1P efflux from erythrocytes by both apoM-dependent and apoM-independent mechanisms. Moreover, apoM facilitates tubular reabsorption of S1P from the urine, however, with no impact on S1P plasma concentrations. PMID: 24950692
    6. The study suggests that vascular leakage of albumin-sized particles in ApoM deficiency is S1P- and S1P1-dependent and this dependency exacerbates the response to inflammatory stimuli. PMID: 26956418
    7. apoM might facilitate the maintenance of CD4(+) T-lymphocytes or could modify the T-lymphocytes subgroups in murine spleen PMID: 26543853
    8. Upon immune stimulation, Apom(-/-) mice developed more severe experimental autoimmune encephalomyelitis, characterized by increased lymphocytes in the central nervous system and breakdown of the blood-brain barrier PMID: 26053123
    9. LDL receptor and ApoE have roles in the clearance of ApoM-associated sphingosine 1-phosphate PMID: 25505264
    10. ApoM augmented insulin secretion by maintaining the S1P concentration under both in vivo and in vitro conditions. PMID: 24814049
    11. The present data indicate that the plasma apo-M levels modulate the ability of plasma to mobilize cellular cholesterol, whereas apo-M has no major effect on the excretion of cholesterol into feces. PMID: 24046869
    12. ApoM can bind oxidized phospholipids, increasing the antioxidant effect of HDL. PMID: 22204862
    13. Results show that apoM, by delivering S1P to the S1P(1) receptor on endothelial cells, is a vasculoprotective constituent of HDL. PMID: 21606363
    14. After refolding from inclusion bodies, the crystal structure of apoM (reported here at 2.5 A resolution) displays a novel yet unprecedented seven-stranded beta-barrel structure. PMID: 20932978
    15. apoM mainly associates with HDL in normal mice but also with the pathologically increased lipoprotein fraction in genetically modified mice; decreased apoM levels in apoA-I-deficient mice suggest a connection between apoM and apoA-I metabolism. PMID: 15102887
    16. ApoM transcripts were detectable in mouse embryos from day 7.5 to day 18.5 PMID: 15147633
    17. in both liver and kidney, expression of apoM was significantly lower in leptin deficient ob/ob mice and in leptin-receptor deficient db/db mice than in control mice PMID: 15358114
    18. Overexpression of apoM in Ldlr(-/-) mice protected against atherosclerosis when the mice were challenged with a cholesterol-enriched diet. PMID: 15793583
    19. Megalin-mediated endocytosis in kidney proximal tubules prevents apoM excretion in the urine. PMID: 16099815
    20. Based on these results, we suggest that insulin regulates apoM synthesis in vivo and, therefore, that the reduction of apoM expression is a general phenomenon in diabetes models. PMID: 16516154
    21. In the setting of low density lipoprotein receptor deficiency, apoM-Tg mice with approximately 2-fold increased plasma apoM concentrations developed smaller atherosclerotic lesions than controls. PMID: 18006500
    22. Apolipoprotein M is a negative acute response protein that decreases during infection and inflammation PMID: 18054359
    23. Foxa2 activity increases plasma high density lipoprotein levels by regulating apolipoprotein M PMID: 18381283

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Calycin superfamily, Lipocalin family
  • 组织特异性：
    Expressed by the liver; secreted in plasma.
  • 数据库链接：

    KEGG: mmu:55938

    STRING: 10090.ENSMUSP00000025249

    UniGene: Mm.2161