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Mouse Apolipoprotein C-III(APOC3) ELISA kit

  • 中文名称：
    小鼠载脂蛋白C-III(APOC3)酶联免疫试剂盒
  • 货号：
    CSB-EL001933MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse APOC3 ELISA Kit was designed for the quantitative measurement of Mouse APOC3 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 18.75 ng/mL-1200 ng/mL and the sensitivity is 9.375 ng/mL.

  • 别名：
    Apoc3Apolipoprotein C-III ELISA kit; Apo-CIII ELISA kit; ApoC-III ELISA kit; Apolipoprotein C3 ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    18.75 ng/mL-1200 ng/mL
  • 灵敏度：
    9.375 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse APOC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 94  
    Range % 90-98  
    1:200 Average % 91  
    Range % 88-95  
    1:400 Average % 83  
    Range % 80-87  
    1:800 Average % 88  
    Range % 84-92  
  • 回收率：
    The recovery of mouse APOC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 92-103  
    EDTA plasma (n=4) 86 82-92  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    1200 0.087 0.089 0.088    
    600 0.188 0.191 0.190    
    300 0.382 0.378 0.380    
    150 0.653 0.645 0.649    
    75 1.131 1.146 1.139    
    37.5 1.516 1.527 1.522    
    18.75 1.819 1.921 1.870    
    0 2.369 2.265 2.317    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Component of triglyceride-rich very low density lipoproteins (VLDL) and high density lipoproteins (HDL) in plasma. Plays a multifaceted role in triglyceride homeostasis. Intracellularly, promotes hepatic very low density lipoprotein 1 (VLDL1) assembly and secretion; extracellularly, attenuates hydrolysis and clearance of triglyceride-rich lipoproteins (TRLs). Impairs the lipolysis of TRLs by inhibiting lipoprotein lipase and the hepatic uptake of TRLs by remnant receptors. Formed of several curved helices connected via semiflexible hinges, so that it can wrap tightly around the curved micelle surface and easily adapt to the different diameters of its natural binding partners.
  • 基因功能参考文献：
    1. Both triglyceride-rich lipoproteins (TRLs) and ApoCIII contribute to the progression of atherosclerosis, and the modulation of TRLs and ApoCIII may represent a novel therapeutic approach against hypertriglyceridemia induced atherosclerosis. PMID: 30223835
    2. C3(QK) variant is a gain-of-function mutation that can stimulate VLDL1 production, through enhanced DNL PMID: 28887372
    3. intestinal apoC-III overexpression results in the secretion of smaller, less dense chylomicron particles along with reduced triacylglycerol secretion from the intestine PMID: 28159868
    4. ApoC-III inhibits turnover of TG-rich lipoproteins primarily through a hepatic clearance mechanism mediated by the LDLR/LRP1 axis PMID: 27400128
    5. These data strongly suggest that intestinal apoC-III is not a FoxO1 target and support the idea that apoC-III is not regulated coordinately with hepatic apoC-III, and establishes another key aspect of apoC-III that is unique in the intestine from the liver. PMID: 28739253
    6. APOC3, whose dysregulation is liable for hypertriglyceridemia, is not a predisposing factor for linking overnutrition to NAFLD in obesity PMID: 28115523
    7. Severe hypertriglyceridaemia resulting from ApoCIII overexpression promotes restenosis and atherosclerosis PMID: 26160324
    8. Under conditions of islet insulin resistance, locally produced apoCIII is an important diabetogenic factor involved in impairment of beta-cell function. PMID: 25941406
    9. decreased ApoAI synthesis might be accounted for the lower plasma HDL level in ApoCIII transgenic mice PMID: 25969427
    10. ApoCIII hyperactivates beta cell CaV1 channels through SR-BI/beta1 integrin-dependent coactivation of PKA and Src. PMID: 23949443
    11. Increased plasma APOC3 concentrations predispose mice to diet-induced nonalcoholic fatty liver and hepatic insulin resistance. PMID: 21793029
    12. Glucose induces apoCIII transcription, which may represent a mechanism linking hyperglycemia, hypertriglyceridemia, and cardiovascular disease in type 2 diabetes. PMID: 21183731
    13. PGC-1beta regulates plasma triglyceride metabolism through stimulating apolipoprotein C3 (APOC3) expression and elevating APOC3 levels in circulation PMID: 20889132
    14. The apoC-III metabolism may contribute to dyslipidemia in CKD, and this requires further investigation. PMID: 19542564
    15. Association between SstI polymorphism of the gene, glucose intolerance and cardiovascular risk in renal transplant recipients PMID: 11959336
    16. ApoB lipoproteins that contain apoCIII increase THP-1 cell adhesion to ECs via PKCalpha and RhoA-mediated beta1-integrin activation. PMID: 16461842
    17. oxidized fatty acids may act through an APOA5/APOClll mechanism that contributes to lowering of TG levels other than PPAR* induction PMID: 18243209
    18. the apoCIII enhancer contributes to the maintenance of an active chromatin subdomain of the apoAI/CIII/AIV genes, but not apoAV PMID: 18678879
    19. These results suggest that apoC-III may play a specific role in lipid storage and mobilization in adipocytes, non-lipoprotein-secreting cells, and indicate the functional role of RXRalpha during adipocyte differentiation. PMID: 19121312

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Apolipoprotein C3 family
  • 数据库链接：