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  4. Mouse Angiopoietin-related protein 7(ANGPTL7) ELISA kit

Mouse Angiopoietin-related protein 7(ANGPTL7) ELISA kit

  • 中文名称：
    小鼠血管生成素相关蛋白7(ANGPTL7)酶联免疫试剂盒
  • 货号：
    CSB-EL001715MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The mouse angiopoietin-related protein 7 (ANGPTL7) ELISA Kit is used to quantitatively measure mouse ANGPTL7 concentrations in serum, plasma, or tissue homogenates. It performs well in important characteristics, including sensitivity and specificity. This assay is based on the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops proportionally to the amount of ANGPTL7 in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.

    ANGPTL7, initially discovered in the stromal layer of the cornea, contributes to coordinating the trabecular meshwork's extracellular matrix and its response to steroids. Levels of ANGPTL7 has been shown to be increased in glaucoma and its overexpression elevates the collagen expression level. While ANGPTL7 induction by glucocorticoids led to the up-regulation of important glaucoma-related proteins including fibronectin, myocilin, and MMP1. ANGPTL7 overexpression has been detected in different human cancers. It exerts roles in various cancers through its interaction with the WNT/-beta-catenin signaling pathway.

  • 别名：
    Angptl7Angiopoietin-related protein 7 ELISA kit; Angiopoietin-like protein 7 ELISA kit
  • 缩写：
    ANGPTL7
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    6.25 pg/mL-400 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANGPTL7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %90
    Range %86-94
    1:2Average %98
    Range %93-103
    1:4Average %102
    Range %95-109
    1:8Average %94
    Range %90-98
  • 回收率：
    The recovery of mouse ANGPTL7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9793-101
    EDTA plasma (n=4)9388-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.758 2.890 2.824 2.632
    2001.823 1.951 1.887 1.695
    1001.077 1.138 1.108 0.916
    500.644 0.621 0.633 0.441
    250.467 0.451 0.459 0.267
    12.50.335 0.336 0.336 0.144
    6.250.262 0.255 0.259 0.067
    00.184 0.199 0.192
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Has a role in the formation and organization of the extracellular matrix. In the eye, it functions as a mediator of dexamethasone-induced matrix deposition in the trabecular meshwork, the tissue responsible for the outflow of the ocular aqueous humor and for the maintenance of intraocular pressure. Is a negative regulator of angiogenesis in the cornea, and plays a major role in maintaining corneal avascularity and transparency.
  • 基因功能参考文献：
    1. Angptl7 is a factor that promotes pro-inflammatory responses in macrophages through the P38 MAPK signaling pathway PMID: 26973239
    2. Angptl7 was indispensable for bone marrow microenvironment to support hematopoietic stem cells repopulation PMID: 25652910
  • 亚细胞定位：
    Secreted.
  • 数据库链接：