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  4. Mouse Angiopoietin-related protein 2(ANGPTL2) ELISA kit

Mouse Angiopoietin-related protein 2(ANGPTL2) ELISA kit

  • 中文名称：
    小鼠血管生成素相关蛋白2(ANGPTL2)酶联免疫试剂盒
  • 货号：
    CSB-EL001710MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse ANGPTL2 ELISA Kit was designed for the quantitative measurement of Mouse ANGPTL2 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 别名：
    Angptl2 ELISA Kit; Arp2Angiopoietin-related protein 2 ELISA Kit; Angiopoietin-like protein 2 ELISA Kit
  • 缩写：
    ANGPTL2
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANGPTL2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %96
    Range %91-102
    1:2Average %102
    Range %98-106
    1:4Average %89
    Range %84-94
    1:8Average %94
    Range %92-96
  • 回收率：
    The recovery of mouse ANGPTL2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 103101-106
    EDTA plasma (n=4)9591-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.221 2.157 2.189 2.032
    5001.510 1.466 1.488 1.331
    2500.896 0.888 0.892 0.735
    1250.502 0.494 0.498 0.341
    62.50.332 0.344 0.338 0.181
    31.20.284 0.295 0.290 0.133
    15.60.211 0.229 0.220 0.063
    00.151 0.163 0.157
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Induces sprouting in endothelial cells through an autocrine and paracrine action.
  • 基因功能参考文献：
    1. ANGPTL2 up-regulation in skeletal myocytes accelerates muscle atrophy, and exercise-induced attenuation of ANGPTL2 expression in those tissues may partially explain how exercise training prevents sarcopenia. PMID: 29191837
    2. Angptl2(-/-) in paraquat-treated mice attenuated acute lung injury progression by reducing the number of total cells, total leukocytes, neutrophils and macrophages in bronchoalveolar lavage fluid and reducing inflammatory response through the inactivation of nuclear factor kappa B pathway. PMID: 29852175
    3. Kaempferol modulates the expression of ANGPTL2 to lessen the mastitis in mice. PMID: 29627690
    4. These results support the importance of ANGPTL2 in the stem cell niche in regulating stemness and epithelial wound healing in the intestine. PMID: 28043948
    5. we provide the first evidence for ANGPTL2 function in the acute ischemic brain. Infiltrated macrophage-derived ANGPTL2 enhanced inflammatory responses and was associated with severe brain tissue damage in the acute phase of ischemic stroke. PMID: 27861531
    6. Angptl2 deficiency in lung epithelial cells and resident alveolar macrophages causes severe lung fibrosis seen following bleomycin treatment, suggesting that ANGPTL2 derived from these cell types plays a protective role against fibrosis in lung. PMID: 27542805
    7. Angptl2 regulates not only adipose tissue metabolism but also bone metabolism PMID: 28285646
    8. findings suggest that macrophage-derived ANGPTL2 promotes an innate immune response in those cells by enhancing proinflammatory activity and NO production required to fight infection. PMID: 27402837
    9. Angptl2 might therefore represent a multistep regulator of CNV pathogenesis and serve as a new therapeutic target for age-related macular degeneration. PMID: 26839315
    10. ANGPTL2 promotes adipose tissue macrophage and T lymphocyte accumulation and leads to insulin resistance. PMID: 26132105
    11. It plays a role in tumor progression. PMID: 25090954
    12. Angptl2 induces proinflammatory responses in peritoneal macrophages and monocytes. PMID: 26435501
    13. In angptl2 knockdown mice, ACh-mediated endothelium-dependent vasodilation was greater than in WT mice. Lack of angptl2 expression limits the metabolic stress induced by a high-fat diet and maintains endothelial function in mice. PMID: 25128474
    14. knockdown of angptl2 may be protective against ANG II-induced cerebral endothelial dysfunction; it favors the production of NO increasing endothelial cell resistance to stress. PMID: 25527773
    15. Angptl2 expression was reduced in PHD3(-/-) bone-marrow-derived macrophages under stress conditions. PHD3 affects the production of Angptl2 and additionally influences the response toward this apoptosis-modulating factor. PMID: 24626957
    16. Angptl2-induced inflammation increases susceptibility to microenvironmental changes, allowing increased oxidative stress and decreased Msh2 expression. PMID: 24258150
    17. Endothelial cell-derived Angptl2 accelerates vascular inflammation by activating proinflammatory signaling in endothelial cells and increasing macrophage infiltration, leading to endothelial dysfunction and atherosclerosis progression. PMID: 24526691
    18. periodic expression of ANGPTL2 is regulated by a molecular clock PMID: 23469106
    19. Our findings demonstrated Angptl2 to be upregulated in corneal inflammation, and highlight that corneal hemangiogenesis and lymphangiogenesis may be driven by Angptk2 overexpression via macrophage infiltration and IL-1beta expression. PMID: 23702783
    20. These findings may provide insight into the molecular mechanisms of the increased expression of Angptl2 and TGF-beta1 in obesity. PMID: 23261458
    21. mediates endotoxin-induced retinal inflammation through the activation of NF-kappaB signaling pathway PMID: 22868908
    22. Macrophage-derived Angptl2 contributes to abdominal aortic aneurysm development by inducing inflammation and degradation of extracellular matrix in the vessel wall. PMID: 22556334
    23. demonstrate the ability of NOS2 to inhibit hyperoxia-induced Ang2 production and thereby decrease Ang2-induced tissue injury PMID: 22227562
    24. tumor cell-derived ANGPTL2 drives metastasis and provided an initial proof of concept for blockade of its action as a strategy to antagonize the metastatic process. PMID: 22345152
    25. keratinocyte-derived Angptl2 functions in dermatomyositis pathogenesis by inducing chronic inflammation in skin tissue. PMID: 22281496
    26. Data suggest that angiopoietin-like protein 2 (Angptl2) contributes to increased carcinogenesis and metastasis and represents a novel target to antagonize these pathologies. PMID: 22042794
    27. Tumor necrosis factor-alpha up-regulates Angptl2 mRNA expression via PI3K/Foxo1 pathway in adipocytes, which may be involved in obesity-induced inflammation and insulin resistance PMID: 21501655
    28. This work pinpoints a specific lymphatic defect of Ang2(-/-) mice and further defines the sequential steps in lymphatic vessel remodeling. PMID: 18514180
    29. Data suggest that interaction of ANG2, a proangiogenic factor, with its receptor Tie2 promotes neural progenitor cell differentiation into neuronal lineage cells. PMID: 19553662
    30. Angiopoietin-like protein 2 (Angptl2) was secreted by adipose tissue and that its circulating level was closely related to adiposity, systemic insulin resistance, and inflammation in mice. PMID: 19723494

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  • 亚细胞定位：
    Secreted.
  • 组织特异性：
    Widely expressed in heart, tongue, lung and skeletal muscle. Also found in lower levels in kidney, epididymis and testis.
  • 数据库链接：