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Mouse Adropin(ENHO) ELISA kit

  • 中文名称：
    小鼠Adropin蛋白(ENHO)酶联免疫试剂盒
  • 货号：
    CSB-EL007669MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse ENHO ELISA Kit was designed for the quantitative measurement of Mouse ENHO protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 pg/mL-20 pg/mL and the sensitivity is 0.078 pg/mL.

  • 别名：
    Enho ELISA Kit; Adropin ELISA Kit; Energy homeostasis-associated protein ELISA Kit
  • 缩写：
    ENHO
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 pg/mL-20 pg/mL
  • 灵敏度：
    0.078 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ENHO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 107    
    Range % 85-112    
    1:2 Average % 103    
    Range % 95-107    
    1:4 Average % 98    
    Range % 89-102    
    1:8 Average % 100    
    Range % 92-104    
  • 回收率：
    The recovery of mouse ENHO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 103 92-106    
    EDTA plasma (n=4) 99 88-103    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    6000 2.232 2.188 2.210 2.044    
    3000 1.677 1.634 1.656 1.490    
    1500 1.213 1.137 1.175 1.009    
    750 0.871 0.844 0.858 0.692    
    375 0.577 0.556 0.567 0.401    
    187.5 0.413 0.425 0.419 0.253    
    93.75 0.287 0.279 0.283 0.117    
    0 0.167 0.165 0.166 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Involved in the regulation of glucose homeostasis and lipid metabolism.
  • 基因功能参考文献：
    1. Adropin prevents atherosclerosis development by suppressing monocyte-endothelial cell adhesion and smooth muscle cell proliferation. PMID: 29701665
    2. An inverse correlation between serum adropin levels and mouse age that was attenuated by calorie restriction. PMID: 28922017
    3. our data suggest that adropin is a membrane-bound protein that interacts with the brain-specific Notch1 ligand NB3. PMID: 25074942
    4. these results suggest a role for adropin in regulating muscle substrate preference under various nutritional states. PMID: 24848071
    5. Release of adropin in the fed condition regulates fuel selection in skeletal muscle, promoting glucose oxidation over fat oxidation. The molecular mechanisms of adropin's effects involve acetylation (suggesting inhibition) of the transcriptional co-activator PGC1alpha, reducing PDK4 and CPT1B activity. Increased PGC1alpha acetylation by adropin may be mediated by inhibiting Sirtuin-1 (SIRT1), a PGC1alpha deacetylase. PMID: 24848071
    6. a potential endothelial protective role of adropin that is likely mediated via upregulation of endothelial NO synthase expression through the VEGFR2-phosphatidylinositol 3-kinase-Akt and VEGFR2-extracellular signal regulated kinase 1/2 pathways. PMID: 20837912
    7. Adropin has an endothelial protective function mediated via upregulation of eNOS expression through the VEGFR2-PI3K-Akt and VEGFR2-ERK1/2 pathways. Adropin therapy may thus be useful for limiting diseases characterized by endothelial dysfunction. PMID: 20837912
    8. Adropin regulated expression of hepatic lipogenic genes and adipose tissue peroxisome proliferator-activated receptor gamma, a major regulator of lipogenesis. PMID: 19041763
    9. Adropin is the name given to the secreted peptide encoded by human C9orf165. In mice, it is abundant in liver where it is regulated by dietary macronutrients. Adropin regulates expression of genes involved in lipogenesis and adipogenesis. PMID: 19041763

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  • 亚细胞定位：
    Secreted.
  • 组织特异性：
    Expressed in liver and brain. Expressed in regions of the brain involved in metabolic regulation.
  • 数据库链接：

    KEGG: mmu:69638

    UniGene: Mm.372459