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  4. Mouse 4-Hydroxynonenal (HNE)ELISA Kit

Mouse 4-Hydroxynonenal (HNE)ELISA Kit

  • 中文名称：
    小鼠4-羟基壬烯酸(HNE)酶联免疫试剂盒
  • 货号：
    CSB-E13412m
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse HNE ELISA Kit was designed for the quantitative measurement of Mouse HNE protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 15.6 pmol/mL-1000 pmol/mL and the sensitivity is 3.9 pmol/mL.

  • 缩写：
    HNE
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma
  • 检测范围：
    15.6 pmol/mL-1000 pmol/mL
  • 灵敏度：
    3.9 pmol/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse HNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %87-98
    1:2Average %96
    Range %91-103
    1:4Average %94
    Range %90-99
    1:8Average %94
    Range %88-98
  • 回收率：
    The recovery of mouse HNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9289-97
    EDTA plasma (n=4)9490-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pmol/mlOD1OD2AverageCorrected
    10002.608 2.555 2.582 2.392
    5001.939 1.897 1.918 1.728
    2501.227 1.212 1.220 1.030
    1250.821 0.759 0.790 0.600
    62.50.468 0.450 0.459 0.269
    31.20.331 0.328 0.330 0.140
    15.60.278 0.271 0.275 0.085
    00.192 0.188 0.190 　
  • 数据处理：
  • 货期：
    3-5 working days

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