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Human γ-Secretase ELISA kit

  • 中文名称：
    人γ分泌酶酶联免疫试剂盒
  • 货号：
    CSB-E13495h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human γ-Secretase ELISA Kit was designed for the quantitative measurement of Human γ-Secretase protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL .

  • 别名：
    gamma-secretase
  • 缩写：
    γ-Secretase
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human γ-Secretase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 86-97  
    1:2 Average % 97  
    Range % 91-103  
    1:4 Average % 95  
    Range % 89-100  
    1:8 Average % 94  
    Range % 89-98  
  • 回收率：
    The recovery of human γ-Secretase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 100 98-101  
    EDTA plasma (n=4) 98 95-100  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2000 2.503 2.458 2.481 2.382  
    1000 2.075 2.088 2.082 1.983  
    500 1.314 1.355 1.335 1.236  
    250 0.741 0.785 0.763 0.664  
    125 0.401 0.386 0.394 0.295  
    62.5 0.312 0.321 0.317 0.218  
    31.25 0.203 0.218 0.211 0.112  
    0 0.096 0.101 0.099 　  
  • 数据处理：
  • 货期：
    3-5 working days

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