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  4. Human tyrosine kinase with immunoglobulin-like and EGF-like domains 1,Tie-1 ELISA kit

Human tyrosine kinase with immunoglobulin-like and EGF-like domains 1,Tie-1 ELISA kit

  • 中文名称：
    人含免疫球蛋白样环和上皮生长因子样域酪氨酸激酶1(Tie-1)酶联免疫试剂盒
  • 货号：
    CSB-E04728h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human TIE1 ELISA Kit was designed for the quantitative measurement of Human TIE1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.94 ng/mL-60 ng/mL and the sensitivity is 0.23 ng/mL.

  • 别名：
    JKT 14 ELISA Kit; JTK14 ELISA Kit; TIE ELISA Kit; Tie1 ELISA Kit; TIE1_HUMAN ELISA Kit; Tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1 ELISA Kit; Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains ELISA Kit; Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 ELISA Kit; Tyrosine protein kinase receptor Tie 1 ELISA Kit; Tyrosine-protein kinase receptor Tie-1 ELISA Kit
  • 缩写：
    TIE1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.94 ng/mL-60 ng/mL
  • 灵敏度：
    0.23 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human Tie-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:5 Average % 87  
    Range % 83-92  
    1:10 Average % 106  
    Range % 101-110  
    1:20 Average % 99  
    Range % 95-103  
    1:40 Average % 98  
    Range % 94-103  
  • 回收率：
    The recovery of human Tie-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 93-99  
    EDTA plasma (n=4) 91 87-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    60 2.531 2.501 2.516 2.402  
    30 2.018 2.121 2.070 1.956  
    15 1.505 1.568 1.537 1.423  
    7.5 1.040 1.068 1.054 0.940  
    3.75 0.657 0.687 0.672 0.558  
    1.88 0.421 0.432 0.427 0.313  
    0.94 0.271 0.290 0.281 0.167  
    0 0.112 0.115 0.114    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.
  • 基因功能参考文献：
    1. We identified colorectal cancer as a novel Tie1-expressing tumor, with Tie1-positive cells hardly detectable in the normal intestine. Tie1 expression did not influence cancer cell proliferation in regular in vitro cultures, but significantly affected malignant growth of transplanted tumors in vivo. PMID: 28464467
    2. T794A-expressing human umbilical vein endothelial cells formed significantly shorter tubes with fewer branches in three-dimensional Matrigel cultures, but did not alter Tie1 or Tie2 tyrosine phosphorylation or downstream signaling. PMID: 26436659
    3. The decreasing expression of Tie1 may play an important role in the pathogenesis of primary lower extremity varicose veins. PMID: 22987233
    4. Data propose that EndMT associated with Tie1 downregulation participates in the pathological development of stroma observed in tumours. PMID: 22421998
    5. The Tie-1 immunoreactivity was dominantly observed in the heamangiogenic cells and cells cords, whereas the matured villi showed immunoreactivity only in other components. PMID: 20164029
    6. A natural antisense transcript was identified for tyrosine kinase containing immunoglobulin and epidermal growth factor homology domain-1 (tie-1), tie-1AS long noncoding RNA in zebrafish, mouse, and humans. PMID: 19880500
    7. high expression independently associated with shorter survival in patients with early chronic phase CML PMID: 11920509
    8. Overexpression and activation of Tie1 is associated with breast and colonic neopalsms PMID: 17786322
    9. the trophoblastic shell of the very early human placenta, as well as endothelial cells and ACC exhibited strong staining intensity for Tie-1 PMID: 18675456
    10. Tie-1 has an inflammatory function in endothelial cells. PMID: 19236867

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, Tie subfamily
  • 组织特异性：
    Specifically expressed in developing vascular endothelial cells.
  • 数据库链接：

    HGNC: 11809

    OMIM: 600222

    KEGG: hsa:7075

    STRING: 9606.ENSP00000361554

    UniGene: Hs.78824