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Human thyrotropin-releasing hormone,TRH ELISA KIT

  • 中文名称：
    人促甲状腺素释放激素(TRH)酶联免疫试剂盒
  • 货号：
    CSB-E08146h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human TRH ELISA Kit was designed for the quantitative measurement of Human TRH protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 2.5 pg/mL-100 pg/mL and the sensitivity is 1.25 pg/mL.

  • 别名：
    TRH ELISA Kit; Pro-thyrotropin-releasing hormone ELISA Kit; Pro-TRH ELISA Kit; Prothyroliberin) [Cleaved into: Thyrotropin-releasing hormone ELISA Kit; TRH ELISA Kit; Protirelin ELISA Kit; TSH-releasing factor ELISA Kit; Thyroliberin ELISA Kit; Thyrotropin-releasing factor ELISA Kit; TRF)] ELISA Kit
  • 缩写：
    TRH
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma
  • 检测范围：
    2.5 pg/mL-100 pg/mL
  • 灵敏度：
    1.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human TRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 86
    Range % 81-92
    1:2 Average % 95
    Range % 85-105
    1:4 Average % 97
    Range % 92-102
    1:8 Average % 93
    Range % 86-98
  • 回收率：
    The recovery of human TRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 94 89-98
    EDTA plasma (n=4) 97 91-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average
    100 2.232 2.252 2.242
    50 1.196 1.156 1.176
    25 0.635 0.648 0.642
    12.5 0.357 0.362 0.360
    5 0.146 0.151 0.149
    2.5 0.075 0.079 0.077
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    As a component of the hypothalamic-pituitary-thyroid axis, it controls the secretion of thyroid-stimulating hormone (TSH) and is involved in thyroid hormone synthesis regulation. It also operates as modulator of hair growth. It promotes hair-shaft elongation, prolongs the hair cycle growth phase (anagen) and antagonizes its termination (catagen) by TGFB2. It stimulates proliferation and inhibits apoptosis of hair matrix keratinocytes.
  • 基因功能参考文献：
    1. TRH rs13097335 may have a protective role toward the development of breast cancer. PMID: 25362934
    2. Data indicate that decrease of cholesterol level impaired the functional coupling between the thyrotropin-releasing hormone receptor and the cognate G proteins. PMID: 25485475
    3. Cpe mutations in mouse may affect processing of prothyrotropin releasing hormone into thyrotropin releasing hormone. PMID: 12270926
    4. TRH inhibits melanin-concentrating hormone (MCH) neurons by increasing synaptic inhibition from local GABA neurons. PMID: 22378876
    5. This study introduces TRH as a novel, potent, selective, and evolutionarily highly conserved neuroendocrine factor controlling human pigmentation in situ. PMID: 21956127
    6. Case Report: Isolated idiopathic central hypothyroidism in an adult, possibly caused by thyrotropin releasing hormone (TRH) deficiency. PMID: 20687402
    7. Thus, human scalp hair follicles are both a source and a target of TRH, which operates as a potent hair-growth stimulator PMID: 19825978
    8. TRH mRNA levels in "mute" thyrotropinomas: cause-effect or coincidence? PMID: 12067454
    9. results show that that leptin can regulate trh gene expression via activation of intracellular signal transducer and activator of transcription 3 (STAT3) proteins in TRH neurons in the hypothalamus PMID: 14764629
    10. The presence of TRH in dysplastic nevi may be predictive for the development of melanoma. PMID: 15328193
    11. Our results support the hypothesis that patients with pseudohypoparathyroidism Ia have impaired sensitivity to both TSH and TRH. PMID: 18805917

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    TRH family
  • 组织特异性：
    Hypothalamus. Expressed in the hair follicle epithelium (at protein level).
  • 数据库链接：

    HGNC: 12298

    OMIM: 613879

    KEGG: hsa:7200

    STRING: 9606.ENSP00000303452

    UniGene: Hs.182231