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  4. Human soluble OX40L (sOX40L) ELISA kit

Human soluble OX40L (sOX40L) ELISA kit

  • 中文名称：
    人可溶性OX40L(sOX40L)酶联免疫试剂盒
  • 货号：
    CSB-E12752h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The human sOX40L ELISA Kit is used to quantitatively measure sOX40L concentrations in human serum, plasma, or tissue homogenates. It performs well in important characteristics, including sensitivity, specificity, precision, recovery, linearity, and lot-to-lot consistency. This assay is based on the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops proportionally to the amount of sOX40L in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.

    OX40L, also called TNFSF4, is a ligand for TNFRSF4/CD40 and is mainly expressed on activated antigen-presenting cells (APCs), such as activated B cells, dendritic cells (DCs), macrophages. The TNFRSF4-TNFSF4 interaction is involved in the control of the helper T-cell-mediated immunity at multiple points, including Th priming, effector cell function, and the generation and maintenance of memory. TNFRSF4-TNFSF4 signaling plays a dual role in T cell responses. It can boost Th1-mediated immune response and promote production and maintenance of Th2 but antagonizes Treg synthesis and Treg-mediated inhibition.

  • 别名：
    TNFSF4; TXGP1; Tumor necrosis factor ligand superfamily member 4; Glycoprotein Gp34; OX40 ligand; OX40L; TAX transcriptionally-activated glycoprotein 1; CD antigen CD252
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human sOX40L in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %106
    Range %100-113
    1:2Average %100
    Range %95-105
    1:4Average %89
    Range %82-98
    1:8Average %97
    Range %92-102
  • 回收率：
    The recovery of human sOX40L spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9387-98
    EDTA plasma (n=4)9490-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.554 2.603 2.579 2.450
    51.975 2.065 2.020 1.891
    2.51.305 1.402 1.354 1.225
    1.250.744 0.714 0.729 0.600
    0.6250.476 0.498 0.487 0.358
    0.3120.351 0.326 0.339 0.210
    0.1560.227 0.226 0.227 0.098
    00.132 0.126 0.129 　
    　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.
  • 基因功能参考文献：
    1. It is concluded that the rs3850641 SNP is significantly associated with the breast cancer susceptibility in Iranian population. PMID: 29949525
    2. TNFSF4 SNPs rs844648, rs844648 and rs704840 were associated with an increased risk of neuromyelitis optica spectrum disorders in Chinese population. PMID: 29032462
    3. The study suggests that the TNFSF4 gene may be involved in the susceptibility to Vogt-Koyanagi-Harada disease and Behcet's Disease in Han Chinese PMID: 27872495
    4. present study suggests a novel association between specific TNFSF4 and BLK gene polymorphisms and allergic rhinitis risk. PMID: 28713926
    5. Data suggest that tumour necrosis factor superfamily 4 (TNFSF4) rs704840 could be the potential systemic lupus erythematosus (SLE) risk factors in the Malaysian population. PMID: 27519474
    6. House dust mite sublingual immunotherapy downregulated Th2-type immune responses mediated by the TSLP-OX40L signaling pathway in patients with persistent moderate to severe allergic rhinitis. PMID: 27012942
    7. OX40 and OX40L formed a functional complex, which may facilitate signal transduction from OX40L to OX40 and contribute to the pathogenesis of Grave's disease. PMID: 27107937
    8. The polymorphisms of the TNFSF4 gene may contribute to the susceptibility to pathogenesis of early-Onset Autoimmune Thyroid Diseases and Hypothyroidism of Hashimoto's Thyroiditis. PMID: 27556446
    9. the SNPs in TNFSF4 and FAM167A-BLK may be involved in asthma and allergic rhinitis gene risk in the Han Chinese cohort. PMID: 27088737
    10. Given the discovery cohort, functional data, and importance of TNFSF4 in infection clearance, TNFSF4C may associate with outcomes and warrants future studies. PMID: 26348892
    11. Review/Meta-analysis: TNFSF4 (rs3850641) polymorphisms is not associated with coronary heart disease risk. PMID: 27008001
    12. Study showed that the A allele of the rs7518045 and haplotype rs3861950C-rs17346501C-rs7518045A-rs1234313G in the TNFSF4 gene were associated with decreased risk for myocardial infarction in a Chinese Han population. PMID: 26125814
    13. higher expression in patients with nasal polyposis compared with patients with chronic rhinosinusitis without nasal polyps PMID: 24583911
    14. contributes to the aberrant T Follicular Helper response in systemic lupus erythematosus PMID: 26070486
    15. Data indicate that soluble OX40 and sOX40L plasma levels are increased in early rheumatoid arthritis patients. PMID: 25359291
    16. Report role of TNFSF4 genetic variants in confering risk of systemic lupus erythematosus in Chinese population. PMID: 24091983
    17. There were no significant associations between rs1234313, rs1234314 and rs17568 and atherosclerotic cerebral infarction risk in a Han Chinese population. PMID: 24595151
    18. Blocking of the interaction between OX40 and OX40L in vitro inhibited production of Thl and Th2 type cytokines in acute renal allograft rejection. PMID: 23953582
    19. TNFSF4 gene polymorphism rs3861950, but not rs3850641, is associated with the risk of atherosclerosis CI in a Chinese population. PMID: 23184501
    20. The results of a meta-analysis suggest that the TNFSF4 rs2205960 polymorphism may confer susceptibility to systemic lupus erythematosus (SLE) in different populations and that the TNFSF4 rs1234315 polymorphism is associated with susceptibility to SLE in Asians. PMID: 22850862
    21. Our data firstly replicated the association of TNFSF4 with renal disorder in SLE patients in the Chinese population, which supported that TNFSF4 may act as a marker of lupus nephritis PMID: 23936824
    22. Our data confirm a global signal at TNFSF4 and a role for the expressed product at multiple stages of lymphocyte dysregulation during SLE pathogenesis PMID: 23874208
    23. The expression levels of OX40 and OX40L were higher in pSS patients. PMID: 23948416
    24. TNFSF4 may have little significance as a common genetic component of Sjogren's syndrome and primary biliary cirrhosis in the Chinese Han population. PMID: 23622253
    25. OX40L expression can be used as a marker to evaluate antiviral treatment efficacy and extend the notion that enhancement of OX40L expression could be a good way for immunotherapy in chronic hepatitis C patients. PMID: 23589118
    26. results suggest that the rs3850641 and rs17568 polymorphisms in the OX40L and OX40 genes are associated with some of the lipid and lipoprotein variations in subjects with endogenous HTG PMID: 23216302
    27. findings provide evidence for the involvement of OX40 and OX40L in the pathogenesis of AD though they are not specific to AD and in vitro results suggest complex interaction PMID: 22646697
    28. The simultaneous expression of both OX40 and its ligand OX40L in idiopathic inflammatory myopathies suggests that they might participate in disease pathogenesis PMID: 23469620
    29. data primarily indicates that rs3850641 of OX40L gene contributes to sporadic breast carcinogenesis in a northeast Chinese Han population. PMID: 22870213
    30. This meta-analysis confirms that TNFSF4 polymorphisms are associated with susceptibility to systemic lupus erythematosus in Asians and Europeans PMID: 22820624
    31. We confirm TNFSF4 as an systemic sclerosis susceptibility gene and rs2205960 as a putative causal variant with preferential association in the ACA+ subphenotype. PMID: 22422496
    32. Concentrations of sOX40L were found to be high in adult asthmatic patients and were associated with the severity of the disease. Therefore, sOX40L could be a potential infl ammatory mediator in the pathogenesis of asthma. PMID: 22760717
    33. Augmentation of OX40-OX40L signals in interferon type I receptor (IFNalphaR)-deficient transgenic mice can substantially enhance CD4+ T cell, but not CD8+ T cell primary responses. PMID: 22156349
    34. High OX40L is associated with acute coronary syndrome and stable angina. PMID: 21986129
    35. Up-regulation of OX40L may play a critical role in development of childhood atopic asthma and correlates with asthma severity. PMID: 21781408
    36. OX40 gene polymorphism may be associated with a risk of ACS in the Han Chinese population, although the association between OX40L polymorphisms and ACS requires further investigation PMID: 21476935
    37. data indicate that the TNFSF4 rs45454293T-allele is associated with lower TNFSF4 expression and increased risk of myocardial infarction PMID: 21445270
    38. Both OX40 upregulation and sOX40L increase were closely associated with Henoch-Schonlein purpura (HSP), especially HSP with nephritis. PMID: 21143648
    39. These results demonstrate for the first time that HDAC11 plays an essential role in regulating OX40L expression. PMID: 21239696
    40. Our study not only suggested that the TNFSF4 gene was associated with systemic lupus erythematosus in the Chinese Han population, but also implied that the TNFSF4 gene might also predispose multiple populations to systemic lupus erythematosus. PMID: 20012871
    41. Data show that MMP-2-conditioned dendritic cells primed naive CD4(+) T cells to differentiate into an inflammatory T(H)2 phenotype through OX40L expression and inhibition of IL-12p70 production. PMID: 21397857
    42. TNFSF4 is a susceptibility gene of coronary heart disease in Chinese Han population PMID: 21402531
    43. The data confirm the influence of TNFSF4 polymorphisms in systemic sclerosis genetic susceptibility, especially in subsets of patients positive for lcSSc and anti-centromere antibodies. PMID: 21187296
    44. our data provide an in vivo role for the OX40/OX40L system in the innate immune response during polymicrobial sepsis PMID: 20844189
    45. Polymorphisms in the TNFSF4 gene region are associated with susceptibility to systemic sclerosis and its clinical and autoantibody subsets. PMID: 19778912
    46. OX40/OX40L expression is increased in the bronchial submucosa in mild asthma, but not in moderate-to-severe disease, and is related to the degree of tissue eosinophilia and IL-4 expression. PMID: 20139223
    47. Expression of CD134 and CD134 ligand in lesional and nonlesional psoriatic skin. PMID: 12624783
    48. combined use of a vector driving the expression of OX40L with three other costimulatory molecules (B7-1, ICAM-1, and LFA-3) both enhances initial activation and then further potentiates sustained activation of nai;ve and effector T cells. PMID: 12798307
    49. An OX40L-dependent mechanism is demonstrated in plasmacytoid dendritic cell-mediated T helper cell responses; OX40L selectively induces Th2-type immune responses by promoting CD4+ T cells to secrete IL-4, IL-5, and IL-13. PMID: 15034038
    50. Elucidation of cross-talk between OX40 and OX40L could be very important in understanding interaction of cells present in inflamed airways of asthma. PMID: 15100674

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  • 相关疾病：
    Systemic lupus erythematosus (SLE)
  • 亚细胞定位：
    Membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Tumor necrosis factor family
  • 数据库链接：

    HGNC: 11934

    OMIM: 152700

    KEGG: hsa:7292

    STRING: 9606.ENSP00000281834

    UniGene: Hs.181097