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  4. Human platelet activating factor,PAF ELISA Kit

Human platelet activating factor,PAF ELISA Kit

  • 中文名称：
    人血小板活化因子(PAF)酶联免疫试剂盒
  • 货号：
    CSB-E07929h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    The Human Platelet Activating Factor (PAF) ELISA Kit is a powerful tool for quantitative detection of PAF in various sample types. PAF, also known as a platelet-activating factor, is a bioactive lipid mediator that plays a crucial role in inflammation and immune response.

    This kit is designed for the detection of human PAF in serum, urine, cell culture supernates, tissue homogenates, and cell lysates. Based on a sandwich assay principle, our kit provides accurate and reproducible results with minimal interference from other proteins.

    With a sensitivity of 1.17 ng/mL and a detection range of 4.69 ng/mL-300 ng/mL, this ELISA kit can accurately measure PAF levels in various sample types. The assay time ranges from 1-5 hours, and the sample volume required is 50-100ul. The detection wavelength is 450 nm, ensuring accurate and precise measurement of PAF levels.

    This PAF ELISA Kit has been cited in more than 8 publications, demonstrating its effectiveness and reliability in research studies. It is an essential tool for researchers studying the role of PAF in various diseases, such as asthma, sepsis, and cardiovascular disease.

  • 别名：
    N/A
  • 缩写：
    PAF
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, urine, cell culture supernates, tissue homogenates, cell lysates
  • 检测范围：
    4.69 ng/mL-300 ng/mL
  • 灵敏度：
    1.17 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Biochemicals
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PAF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %85
    Range %81-92
    1:2Average %95
    Range %90-105
    1:4Average %94
    Range %91-102
    1:8Average %90
    Range %80-98
  • 回收率：
    The recovery of human PAF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9188-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    3002.097 2.093 2.095 1.994
    1501.823 1.793 1.808 1.707
    751.476 1.374 1.425 1.324
    37.51.029 0.986 1.008 0.907
    18.750.782 0.772 0.777 0.676
    9.380.421 0.401 0.411 0.310
    4.690.218 0.202 0.210 0.109
    00.105 0.097 0.101 　
  • 本试剂盒所含材料：
    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human PAF antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled PAF antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-conjugateDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

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