Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Human phosphoglycerate mutase 2,PGAM2 ELISA Kit

Human phosphoglycerate mutase 2,PGAM2 ELISA Kit

  • 中文名称：
    人磷酸甘油酸变位酶2(PGAM2)酶联免疫试剂盒
  • 货号：
    CSB-E11282h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human PGAM2 ELISA Kit was designed for the quantitative measurement of Human PGAM2 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 别名：
    BPG dependent PGAM 2 ELISA Kit; BPG-dependent PGAM 2 ELISA Kit; GSD10 ELISA Kit; MGC88743 ELISA Kit; Muscle specific phosphoglycerate mutase ELISA Kit; Muscle-specific phosphoglycerate mutase ELISA Kit; OTTHUMP00000207787 ELISA Kit; PGAM 2 ELISA Kit; PGAM M ELISA Kit; PGAM-M ELISA Kit; Pgam2 ELISA Kit; PGAM2_HUMAN ELISA Kit; PGAMM ELISA Kit; phosphoglycerate mutase 2 (muscle) ELISA Kit; Phosphoglycerate mutase 2 ELISA Kit; Phosphoglycerate mutase isozyme M ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PGAM2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 88  
    Range % 82-94  
    1:2 Average % 102  
    Range % 98-105  
    1:4 Average % 95  
    Range % 89-99  
    1:8 Average % 98  
    Range % 91-102  
  • 回收率：
    The recovery of human PGAM2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-98  
    EDTA plasma (n=4) 98 92-104  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.526 2.417 2.472 2.327  
    500 1.608 1.505 1.557 1.412  
    250 0.884 0.855 0.870 0.725  
    125 0.486 0.494 0.490 0.345  
    62.5 0.329 0.306 0.318 0.173  
    31.2 0.265 0.257 0.261 0.116  
    15.6 0.197 0.189 0.193 0.048  
    0 0.145 0.144 0.145    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase), but with a reduced activity.
  • 基因功能参考文献：
    1. These results reveal a mechanism of PGAM2 regulation and NADPH homeostasis in response to oxidative stress that impacts cell proliferation and tumor growth. PMID: 24786789
  • 相关疾病：
    Glycogen storage disease 10 (GSD10)
  • 蛋白家族：
    Phosphoglycerate mutase family, BPG-dependent PGAM subfamily
  • 组织特异性：
    Expressed in the heart and muscle. Not found in the liver and brain.
  • 数据库链接：

    HGNC: 8889

    OMIM: 261670

    KEGG: hsa:5224

    STRING: 9606.ENSP00000297283

    UniGene: Hs.632642