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  4. Human peroxisome proliferative activated receptor gama coactivator 1 alpha,PPARGC1 ELISA Kit

Human peroxisome proliferative activated receptor gama coactivator 1 alpha,PPARGC1 ELISA Kit

  • 中文名称：
    人过氧化物酶体增殖激活物受体γ辅激活因子1α(PPARGC1)酶联免疫试剂盒
  • 货号：
    CSB-E11761h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This human PPARGC1A ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human PPARGC1A in different samples, including serum, plasma, cell culture supernates, or cell lysates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of PPARGC1A bound in the initial step.

    PPARGC1A is involved in biological functions with implications for insulin action including protection against oxidative stress, formation of muscle fiber types as well as regulation of microvascular flow. It is a chief regulator of energy metabolism and mitochondrial biogenesis by integrating and coordinating the activity of other transcription factors such as Nrf1, nuclear factor 2, PPARα, and mitochondrial transcription A. PPARGC1A is also central to antioxidant defense and redox balance, by regulating the expression of factors such as Nrf2 and MnSOD and promoting NADPH generation, and thereby counters increased ROS levels in highly oxidative cells and protects against inflammation. It is involved in the progression of hormone-associated cancers.

  • 别名：
    L PGC 1alpha ELISA Kit; LEM6 ELISA Kit; Ligand effect modulator 6 ELISA Kit; Peroxisome proliferative activated receptor gamma coactivator 1 alpha ELISA Kit; Peroxisome proliferative activated receptor gamma coactivator 1 ELISA Kit; Peroxisome proliferator activated receptor gamma coactivator 1 alpha ELISA Kit; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 3ext ELISA Kit; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 8a ELISA Kit; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 ELISA Kit; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5 ELISA Kit; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5 NT ELISA Kit; Peroxisome proliferator-activated receptor gamma coactivator 1-alpha ELISA Kit; PGC 1 (alpha) ELISA Kit; PGC 1 alpha ELISA Kit; PGC 1v ELISA Kit; PGC-1-alpha ELISA Kit; PGC1 ELISA Kit; PGC1(alpha) ELISA Kit; PGC1A ELISA Kit; PGC1v ELISA Kit; PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6 ELISA Kit; PPAR gamma coactivator 1 alpha ELISA Kit; PPAR gamma coactivator 1 ELISA Kit; PPAR gamma coactivator variant form ELISA Kit; PPAR-gamma coactivator 1-alpha ELISA Kit; PPARGC 1 alpha ELISA Kit; PPARGC-1-alpha ELISA Kit; PPARGC1 ELISA Kit; PPARGC1A ELISA Kit; PRGC1_HUMAN ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, cell lysates
  • 检测范围：
    125 pg/mL-8000 pg/mL
  • 灵敏度：
    31.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PPARGC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %84
    Range %80-92
    1:2Average %97
    Range %90-105
    1:4Average %99
    Range %92-110
    1:8Average %95
    Range %86-99
  • 回收率：
    The recovery of human PPARGC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-100
    EDTA plasma (n=4)9690-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    80002.054 2.052 2.053 1.954
    40001.315 1.325 1.320 1.221
    20000.762 0.777 0.770 0.671
    10000.470 0.477 0.474 0.375
    5000.284 0.295 0.290 0.191
    2500.216 0.224 0.220 0.121
    1250.157 0.165 0.161 0.062
    00.096 0.102 0.099 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK.
  • 基因功能参考文献：
    1. LL was significantly negatively correlated with PGC-1alpha, TNF-alpha, and IL-6 mRNA expressions. PGC-1alpha mRNA expression levels in paraspinal muscles may be affected by lumbar kyphosis. PMID: 30233161
    2. PGC1A can improve mitochondrial biogenesis and negatively regulate senescence, although this function of PGC1A in age-related macular degeneration needs further studies. Post-translational modifications of PGC1A by AMPK (AMP kinase) and SIRT1 (sirtuin 1) are crucial for its activation and important in age-related macular degeneration pathogenesis. PMID: 30087287
    3. study provides support for the involvement of both NFE2L2 and PPARGC1alpha in Parkinson's disease susceptibility and progression, marginally and through pathways involving maneb and paraquat exposure. PMID: 29630901
    4. Increased nuclear p53 phosphorylation and PGC-1alpha protein content immediately following SIE but not CE suggests these may represent important early molecular events in the exercise-induced response to exercise PMID: 28281651
    5. The transcriptional co-activator peroxisome proliferator-activated receptor gamma co-activator-1 alpha (PGC-1alpha) is proposed to coordinate skeletal muscle mitochondrial biogenesis through the integrated induction of nuclear- and mitochondrial-encoded gene transcription--{REVIEW} PMID: 29126696
    6. The changes of epigenetic modification of PGC-1alpha gene in pregnant women with gestational diabetes mellitus may be a mechanism of abnormal glucose metabolism in offspring. PMID: 29730292
    7. Our results showed that administration of Hcy reduced the SIRT1/AMPK/PGC-1alpha signaling in chondrocytes, leading to mitochondrial dysfunction as a result of increased oxidative stress and apoptosis PMID: 29413962
    8. Study in melanoma cell and in vivo melanoma models provides evidence for a direct role of BRD4 binding at super-enhancers that drive the expression of PGC-1alphaalpha and SOX10, a transcription factor involved in melanocyte development. PMID: 28991225
    9. the present study provided evidence that cisplatin stimulated the expression of PGC1A and the upregulation of mitochondrial biogenesis through PGC1A, promoting cell viability and inhibiting apoptosis in response to cisplatin treatment, thus triggering cisplatin resistance in ovarian cancer cells. PMID: 29749474
    10. At least in the subcutaneous fat of humans and mice, the levels of PGC-1a decrease during obesity, while its physical association with A20 increases. PMID: 29678181
    11. The non-responders for aerobic training are the carriers of PPARGC1A rs8192678 Ser/Ser. The best responders to aerobic training are PPARGC1A rs8192678 Gly/Gly carriers. PMID: 29762540
    12. Pathological cardiac hypertrophy, decrease of PGC-1alpha activity, and reactivation of the fetal genetic program in chronic heart failure are demonstrated. PMID: 29658071
    13. The findings suggest that activation of TGR5 promoted mitochondrial biogenesis in endothelial cells, which is mediated by the CREB/PGC-1a signaling pathway. PMID: 29709472
    14. that PGC-1alpha may be useful for predicting nodal metastasis, and may represent a biomarker for poor prognosis in colorectal cancer PMID: 29566941
    15. Koreans with the minor alleles of PGC-1alpha rs10517030, rs10517032 and rs10212638 are at greater risk of type 2 diabetes mellitus. PMID: 28488691
    16. It was found that down-regulated PGC-1alpha expression is independently associated with the development of CVD in HD patients. PMID: 28550474
    17. Results provide evidence that PGC-1A regulates FASN expression level in colorectal cancer cells. PMID: 29130104
    18. PPARGC1A expression is differentially regulated via canonical and alternative promoters in trained and untrained skeletal muscle. PMID: 29233908
    19. the results reveal that PGC-1alpha stimulates bioenergetic potential, which promotes breast cancer metastasis and facilitates adaptation to metabolic drugs. PMID: 28988825
    20. No associations were observed between SNPs in PPARGC1 and obesity. PMID: 28947843
    21. Results show that PGC1 function is modulated by ERRa which physically interacts with PGC1a, promoting the expression of genes involved in mitochondrial oxidative phosphorylation, but does not affect expression of the cellular antioxidant or invasive/metastatic programs. PMID: 28596418
    22. PGC-1alpha induction during differentiation is required for both mitochondrial biogenesis and commitment to the hepatocytic lineage, and TCF7L2 repression is sufficient to increase PGC-1alpha expression, mitochondrial biogenesis and OXPHOS activity. PMID: 28795454
    23. AMPK-PGC-1a control of mitochondrial reactive oxygen species regulates Warburg metabolism. PMID: 28978464
    24. Our results suggest that maternal inflammation programmed proneness to NF-kappaB over-activation which impaired PGC-1a-mediated anti-oxidant capacity resulting in the increased sensitivity of offspring to hypertensive damage. PMID: 27616627
    25. Postexercise cold water immersion of one limb also enhances PGC-1alpha expression. PMID: 28546467
    26. Multiple regression analysis showed that age, male gender, body max index, presence of obesity, type-2-diabetes mellitus, hypertension and coronary artery disease and left ventricular ejection fraction were associated with the expression levels of UCP1, PGC1alpha and PRDM16 mRNA PMID: 28824327
    27. This is the first study regarding the relationship of PPARGC1A and ADIPOQ polymorphisms, and the aggressiveness of prostate cancer in men with overweight or obesity. PMID: 28453464
    28. The effects of PGC-1 gene Gly482Ser polymorphisms on alterations in glucose and lipid metabolism induced by exercise training. PMID: 27699582
    29. acute upregulation of PGC-1alpha mRNA relates to the magnitude of subsequent training-induced increases in oxidative capacity, but not other molecular and morphological chronic skeletal muscle adaptations PMID: 28177701
    30. Data show a significant differential pattern of genetic association of peroxisome proliferator-activated receptor gamma, coactivator 1 alpha (PGC-1alpha) among ethnic groups emphasizing the role of ethnicity towards disease susceptibility [Meta-analysis]. PMID: 29063962
    31. Findings suggest that despite their proposed involvement in the regulation of inflammatory processes in multiple sclerosis (MS), PPARalpha, PPARbeta/delta, and PGC-1alpha proteins are not potential biomarkers of neuroinflammation in MS, and indicate a preferential role of PPARgamma in the endogenous regulation of autoimmune response in the human central nervous system within its receptor family. PMID: 28483651
    32. this study shows that PPARGC1A rs3736265 G>A polymorphism is associated with decreased risk of type 2 diabetes mellitus and fasting plasma glucose PMID: 28418876
    33. Suggest that a pro-inflammatory diet may have a differential effect on colorectal cancer risk based on PPARGC1A genetic variation. PMID: 28051997
    34. that oxidatively-mediated reductions in parkin solubility and function in a mouse model of age-related sporadic PD coincides with increased PARIS levels and reduced PGC-1alpha signaling PMID: 27185595
    35. Study found that the metabolic master regulator PGC-1alpha is differentially affected by ALS-associated mutations in brain vs. peripheral tissues. Increased PGC-1alpha activity in peripheral tissue contributes to the metabolic phenotype, while in the CNS blunting of the PGC-1alpha response renders motoneurons vulnerable. PMID: 27818323
    36. A single nucleotide variant of the PPARGC1A gene (rs8192678) is associated with T2D susceptibility, relative risk of obesity and insulin resistance, and lower indices of beta cell function. This common polymorphism is within a highly conserved region of the bioactive protein and leads to a single amino acid substitution (glycine 482 to serine).[review] PMID: 29186342
    37. We aimed to explore the potential therapeutic effect of PGC-1alpha by generating a lentiviral vector to express human PGC-1alpha and target it by stereotaxic delivery to hippocampus and cortex of APP23 transgenic mice . Four months after hPGC-1alpha injection, mice showed improved spatial and recognition memory concomitant with a significant reduction in Abeta deposition, associated with a decrease in BACE1 expression PMID: 27791018
    38. In this review, we discuss the interplay between PGC-1 coactivators and cancer pathogenesis, including tumor initiation, metastatic spread, and response to treatment. Given their involvement in the functional biology of cancers, identification of regulatory targets that influence PGC-1 expression and activity may reveal novel strategies suitable for mono- or combinatorial cancer therapies. [Review] PMID: 28607951
    39. Physiologic plasma FFA elevation in NGT individuals has opposing effects on PGC-1alpha non-CpG residue methylation (CpT demethylation and increased DNMT-3B expression), which is correlated with changes in PGC-1alpha expression and skeletal muscle mitochondrial function PMID: 29261667
    40. IFNgamma induced PPAR gamma coactivator-1 alpha (PGC-1alpha) positively regulated RIG-I; with PRMT-1 and G9a affecting PGC-1alpha in a counter-regulatory manner. PMID: 26725954
    41. These results show the importance of the activation of the MC1R-PGC-1alpha pathway for mitochondrial biogenesis and function in melanoma development, as well as BRAF for the antioxidant response regulated by PGC-1alpha. PMID: 28452072
    42. Postexercise expression of PGC-1alpha gene via the alternative promoter was not affected. PMID: 28074493
    43. The present study investigated the neuroprotective effects and signal transduction mechanisms of the overexpression of PGC-1alpha on N-methyl-4-phenylpyridinium ion (MPP(+))-induced mitochondrial damage in SH-SY5Y cell. PMID: 26141122
    44. These data indicate that acute exercise in a hot environment blunts expression of mitochondrial biogenesis-related mRNA, due to decreased binding of CREB, MEF2, and FoxO1 to the PGC-1alpha promoter. PMID: 27445305
    45. skeletal muscle PGC-1alpha is required for fasting-induced upregulation of skeletal muscle SIRT3 and maintaining high fat oxidation in the fasted state, but is dispensable for preserving the capability to switch substrate during the transition from the fed to the fasted state and for fasting-induced PDH regulation in skeletal muscle. PMID: 28400503
    46. Conversely, nitric oxide (NO) triggered post translation modifications of PGC-1alpha and induced FAO, recovering the bioenergetics impairment of muscles but shunting the defective mitochondrial biogenesis. PMID: 27974213
    47. The results revealed that the distribution of genotypes and allele frequency of the PGC-1alpha Gly482Ser polymorphism in polycystic ovary syndrome patients was statistically significant from those of the control group respectively indicating that the presence of 'A' allele might confer risk to polycystic ovary syndrome. PMID: 29030253
    48. mitochondrial biogenesis using mdivi1 or by silencing PGC1alpha abrogates differentiation of Glioblastoma multiforme differentiation into astroglia; conversely, overexpression of PGC1alpha elicits differentiation. PMID: 28076790
    49. PGC-1alpha protein was higher after HIHVT than after SIT (p < 0.05). Moreover, the AMPKpTHR172/AMPK ratio increased at post after SIT (p < 0.05), whereas this effect was delayed after HIHVT as it increased after 3 h PMID: 28973039
    50. study demonstrated that PGC1alpha serves as a unique adaptor molecule for the recruitment of additional coactivator proteins, which can finely regulate HBV (Hepatitis B Virus) transcription PMID: 28768874

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  • 亚细胞定位：
    [Isoform 1]: Nucleus. Nucleus, PML body.; [Isoform B4]: Nucleus.; [Isoform B4-8a]: Cytoplasm. Nucleus.; [Isoform B5]: Nucleus. Nucleus, PML body.; [Isoform 9]: Nucleus.
  • 组织特异性：
    Heart, skeletal muscle, liver and kidney. Expressed at lower levels in brain and pancreas and at very low levels in the intestine and white adipose tissue. In skeletal muscle, levels were lower in obese than in lean subjects and fasting induced a 2-fold i
  • 数据库链接：

    HGNC: 9237

    OMIM: 604517

    KEGG: hsa:10891

    STRING: 9606.ENSP00000264867

    UniGene: Hs.527078