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中文名称:人过氧化物酶2(PRDX2)酶联免疫试剂盒
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货号:CSB-EL018654HU
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规格:96T/48T
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价格:¥3600/¥2500
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其他:
产品详情
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产品描述:
This Human PRDX2 ELISA Kit was designed for the quantitative measurement of Human PRDX2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 47 pg/mL-3000 pg/mL and the sensitivity is 11.75 pg/mL .
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缩写:
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Uniprot No.:
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种属:Homo sapiens (Human)
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样本类型:serum, plasma, tissue homogenates, cell lysates
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检测范围:47 pg/mL-3000 pg/mL
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灵敏度:11.75 pg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Cell Biology
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human PRDX2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 85 Range % 81-95 1:2 Average % 97 Range % 91-105 1:4 Average % 96 Range % 92-108 1:8 Average % 93 Range % 86-102 -
回收率:
The recovery of human PRDX2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 92 89-98 EDTA plasma (n=4) 97 92-100 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 3000 2.381 2.419 2.400 2.253 1500 1.935 1.952 1.944 1.797 750 1.427 1.403 1.415 1.268 375 1.068 1.059 1.064 0.917 187.5 0.627 0.65 0.639 0.492 94 0.412 0.423 0.418 0.271 47 0.295 0.28 0.288 0.141 0 0.149 0.145 0.147 -
数据处理:
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货期:3-5 working days
引用文献
- Proteomics changes after negative pressure wound therapy in diabetic foot ulcers Z Jia,Molecular medicine reports,2021
靶点详情
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功能:Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).
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亚细胞定位:Cytoplasm.
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蛋白家族:Peroxiredoxin family, AhpC/Prx1 subfamily
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数据库链接:
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