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  4. Human parathyroid hormone 1-34(PTH 1-34)antibody ELISA kit

Human parathyroid hormone 1-34(PTH 1-34)antibody ELISA kit

  • 中文名称：
    人甲状旁腺激素1-34(PTH 1-34)抗体酶联免疫试剂盒
  • 货号：
    CSB-EQ027274HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The human parathyroid hormone 1-34 (PTH 1-34) antibody ELISA kit allows for the qualitative identification of PTH 1-34 antibodies in human serum and plasma. It employs the qualitative enzyme immunoassay technique. The microtiter plate has been pre-coated with the PTH 1-34. Samples or standards are pipetted into the wells with anti-human immunoglobulin conjugated HRP. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of human PTH 1-34 antibody bound in the initial step. The color development is stopped and the intensity of the color is measured by a microplate reader at 450 nm. The PTH 1-34 antibody titer in the samples is determined by referring to the negative control. It indicates the presence of PTH 1-34 antibody if the O.D. (optical density) of PTH 1-34 antibody is greater than or equal to the cutoff value(2.1× Average O.D. value of negative control). There is no PTH 1-34 antibody present in the sample if the O.D. is less than the cutoff value.

    This assay has excellent specificity for the detection of PTH 1-34 antibodies. And it also has been validated with precision less than 15% and lot-to-lot consistency. Get more details from the product instructions.

    PTH 1-34 is a type of parathyroid hormone approved for the treatment of osteoporosis in postmenopausal women and in men who are at high risk of breaking a bone. It contributes to rebuilding bone and significantly increasing bone mineral density.

  • 缩写：
    PTH 1-34 Ab
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma
  • 检测范围：
    Request Information
  • 灵敏度：
    Request Information
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    qualitative
  • 测定方法：
    Indirect
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 标准曲线：
                 
                 
    Test parameter specification test result  
    Positive control >0.8 1.895  
    Negative control <0.2 0.135  
    Positive rate 10,Positive 100%  
    Negative rate 10,Negative 100%  
                       
  • 本试剂盒所含材料：
    • A 96-well Assay plate --The 96-well plate has been pre-coated with PTH 1-34.
    • Negative Control (1 x 800 μl) --Eliminate false positive
    • Positive Control (1 x 800 μl) --Used to evaluate the validity, stability, and comparability of experimental results.
    • Sample Diluent (2x 20 ml) --Dilute the sample to an appropriate concentration.
    • HRP-conjugated Immunoglobulin (1 x 10ml)--Bind to the PTH 1-34 antibody and HRP catalyzes the TMB to elicit a chromogenic reaction.
    • HRP-conjugate Diluent (2x 20 ml) --(1 x 20ml)
    • Wash Buffer (25x concentrate) (1 x 20 ml) --Wash away unbound or free substances.
    • Substrate A (1 x 5 ml) --Mix with substrate B and interact with HRP, eliciting a Chromogenic reaction.
    • TMB Substrate (1 x 10ml) --Interact with HRP, eliciting a Chromogenic reaction.
    • Stop Solution (1 x 10 ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells)--Cover the microplate when incubating.
    • An Instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 货期：
    3-5 working days

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问答及客户评论

■ 常见问题解答
Q:

I am working with a PTH compound that is smaller than 1-34 so I was wondering if you could provide the immunogen that was used to generate the detection ab? Is the epitope for the detection antibody in the 7-33 range?

A:
Thanks for your inquiry!
CSB-EQ027274HU detect the PTH 1-34 antibody.
According to the description from this customer, the user needs kit for detecting antigen （<1-34 AA), the recognition area is 7-33.
So sorry that this kit cannot match the customer's request. We only have this kit for intact PTH.
Appreciated to your kind understanding.