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Human osteocrin,Ostn ELISA kit

  • 中文名称：
    人骨织素(Ostn)酶联免疫试剂盒
  • 货号：
    CSB-E12021h
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human OSTN ELISA Kit was designed for the quantitative measurement of Human OSTN protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .

  • 别名：
    OSTN ELISA Kit; Osteocrin ELISA Kit; Musclin) [Cleaved into: Processed Osteocrin] ELISA Kit
  • 缩写：
    OSTN
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Stem Cells
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human Ostn in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %87
    Range %82-92
    1:2Average %99
    Range %94-103
    1:4Average %96
    Range %92-99
    1:8Average %95
    Range %88-98
  • 回收率：
    The recovery of human Ostn spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-97
    EDTA plasma (n=4)9791-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.552 2.699 2.626 2.510
    102.254 2.373 2.314 2.198
    51.804 1.928 1.866 1.750
    2.50.889 0.926 0.908 0.792
    1.250.517 0.498 0.508 0.392
    0.6250.320 0.304 0.312 0.196
    0.3120.216 0.202 0.209 0.093
    00.118 0.114 0.116 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Hormone that acts as a regulator of dendritic growth in the developing cerebral cortex in response to sensory experience. Induced in the brain following membrane depolarization and inhibits dendritic branching in neurons of the developing cortex. Probably acts by binding to natriuretic peptide receptor NPR3/NPR-C, thereby preventing binding between NPR3/NPR-C and natriuretic peptides, leading to increase cGMP production.
  • 基因功能参考文献：
    1. The circulating concentration of musclin was significantly increased in type 2 diabetes mellitus patients. Results suggest that musclin has a strong relationship with insulin resistance in type 2 diabetes mellitus. PMID: 28185530
    2. findings suggest that, in response to sensory input, OSTN regulates features of neuronal structure and function that are unique to primates PMID: 27830782
    3. osteocrin represents a novel, unique vitamin D-regulated bone-specific protein that appears to act as a soluble osteoblast regulator PMID: 14523025
    4. Foxo1 downregulates musclin mRNA expression both in vitro and in vivo, which should explain insulin-mediated upregulation of this gene in muscle cells. PMID: 17950246
    5. Ostn is a naturally occurring ligand of the NPR-C clearance receptor and may act to locally modulate the actions of the natriuretic system in bone by blocking the clearance action of NPR-C, thus locally elevating levels of C-type natriuretic peptide. PMID: 17951249
    6. Observational study of gene-disease association. (HuGE Navigator) PMID: 19453261

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Osteocrin family
  • 组织特异性：
    Enriched in neocortical regions of the developing cerebral cortex. Not expressed in other compartments of the neocortical wall or in brain regions such as the hippocampus, striatum, mediodorsal nucleus of the thalamus and cerebellum. Also expressed in bon
  • 数据库链接：

    HGNC: 29961

    OMIM: 610280

    KEGG: hsa:344901

    STRING: 9606.ENSP00000342356

    UniGene: Hs.526794