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  4. Human neuromedin U (NMU) ELISA kit

Human neuromedin U (NMU) ELISA kit

  • 中文名称：
    人神经调节肽U(NMU)酶联免疫试剂盒
  • 货号：
    CSB-EL015902HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human NMU ELISA Kit was designed for the quantitative measurement of Human NMU protein in serum, plasma, cerebrospinal fluid (CSF), tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

  • 别名：
    Neuromedin U 25 ELISA Kit; Neuromedin U ELISA Kit; Neuromedin-U-25 ELISA Kit; NmU 25 ELISA Kit; NMU ELISA Kit; NmU-25 ELISA Kit; NMU_HUMAN ELISA Kit
  • 缩写：
    NMU
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cerebrospinal fluid (CSF), tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of Human NMU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %92
    Range %84-98
    1:2Average %92
    Range %86-98
    1:4Average %94
    Range %84-98
    1:8Average %94
    Range %88-98
  • 回收率：
    The recovery of Human NMU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9892-102
    EDTA plasma (n=4)8884-94
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.585 2.387 2.486 2.314
    102.058 1.896 1.977 1.805
    51.375 1.304 1.340 1.168
    2.50.852 0.879 0.866 0.694
    1.250.574 0.552 0.563 0.391
    0.6250.369 0.384 0.377 0.205
    0.3120.271 0.265 0.268 0.096
    00.172 0.171 0.172
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Ligand for receptors NMUR1 and NMUR2. Stimulates muscle contractions of specific regions of the gastrointestinal tract. In humans, NmU stimulates contractions of the ileum and urinary bladder.; Does not function as a ligand for either NMUR1 or NMUR2. Indirectly induces prolactin release although its potency is much lower than that of neuromedin precursor-related peptide 36.; Does not function as a ligand for either NMUR1 or NMUR2. Indirectly induces prolactin release from lactotroph cells in the pituitary gland, probably via the hypothalamic dopaminergic system.
  • 基因功能参考文献：
    1. Results identified NMU as highly expressed in non-small cell lung cancer (NSCLC) tissues and cell lines. Furthermore, integrative bioinformatics analyses demonstrate that NMU may confer the alectinib resistance in NSCLC via multiple mechanisms. PMID: 30096454
    2. NMU might contribute to progression of NMUR2-positive breast cancer PMID: 28423716
    3. The anti-tumorigenic effect of HAND2-AS1 in endometrioid endometrial carcinoma was mediated by down-regulating neuromedin U expression. PMID: 29107108
    4. Results indicate a mechanism of action for neuromedin U (NmU) in HER2-overexpressing breast cancer that enhances resistance to HER2-targeted drugs through conferring cancer stem cell (CSC) characteristics and expansion of the CSC phenotype. PMID: 28340506
    5. This study shows an association between a NMU haplotype and anthropometric indices, mainly linked to fat mass, which appears to be age- and sex-specific in children. PMID: 28235053
    6. Overexpression of Nmu may be involved in the process of regional metastasis of HNSCC, and may serve as a novel and valuable biomarker for predicting regional metastasis in patients with HNSCC. PMID: 27279246
    7. polymorphisms in the gene may be useful in identifying women at risk for osteoporosis. PMID: 25871004
    8. our results defined NmU as a candidate drug response biomarker for HER2-overexpressing cancers and as a candidate therapeutic target to limit metastatic progression and improve the efficacy of HER-targeted drugs. PMID: 24876102
    9. a role for NMU gene through interaction with ADRB2 gene in bone strength regulation PMID: 23936460
    10. Inactivation of the von Hippel-Lindau tumour suppressor gene induces Neuromedin U expression in renal cancer cells. PMID: 21791076
    11. [review] Taken together with its vascular actions, NMU may be a functional link between energy balance and the cardiovascular system and may provide a future target for therapies directed against the disorders that comprise metabolic syndrome. PMID: 19519756
    12. These results suggest that NMU plays a role in feeding behavior and catabolic functions via corticotropin-releasing hormone. PMID: 14623274
    13. NmU expression is related to Myb and that the NmU/NMU1R axis constitutes a previously unknown growth-promoting autocrine loop in myeloid leukemia cells PMID: 15187020
    14. irreversible binding of NmU to its receptors PMID: 15331768
    15. Overexpression of neuromedin U is associated with bladder tumor formation, lung metastasis and cancer cachexia PMID: 16878152
    16. Amino acid variants in NMU associate with overweight and obesity, suggesting that NMU is involved in energy regulation in humans. PMID: 16984985
    17. NMU & its cancer-specific receptors, as well as its target genes, are frequently overexpressed in clinical samples of lung cancer and in cell lines, and that those gene products play indispensable roles in the growth and progression of lung cancer cells. PMID: 17018595
    18. NmU may be involved in the HGF-c-Met paracrine loop regulating cell migration, invasiveness and dissemination of pancreatic ductal adenocarcinoma. PMID: 19118941

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    NmU family
  • 组织特异性：
    Expressed throughout the enteric nervous system with highest levels being found in the jejunum.
  • 数据库链接：

    HGNC: 7859

    OMIM: 605103

    KEGG: hsa:10874

    STRING: 9606.ENSP00000264218

    UniGene: Hs.418367