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  4. Human mucin-5AC (MUC5AC) ELISA kit

Human mucin-5AC (MUC5AC) ELISA kit

  • 中文名称：
    人粘蛋白5AC(MUC5AC)酶联免疫试剂盒
  • 货号：
    CSB-E10109h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human MUC5AC ELISA Kit was designed for the quantitative measurement of Human MUC5AC protein in serum, plasma, cell culture supernates, urine. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL .

  • 别名：
    MUC5AC ELISA Kit; MUC5 ELISA Kit; Mucin-5AC ELISA Kit; MUC-5AC ELISA Kit; Gastric mucin ELISA Kit; Major airway glycoprotein ELISA Kit; Mucin-5 subtype AC ELISA Kit; tracheobronchial ELISA Kit; Tracheobronchial mucin ELISA Kit; TBM ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, urine
  • 检测范围：
    15.6 ng/mL-1000 ng/mL
  • 灵敏度：
    3.9 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human MUC5AC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %95
    Range %87-99
    1:2Average %94
    Range %87-96
    1:4Average %98
    Range %94-106
    1:8Average %90
    Range %86-97
  • 回收率：
    The recovery of human MUC5AC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8795-93
    EDTA plasma (n=4)9491-97
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    10002.452 2.563 2.508 2.375
    5001.949 1.964 1.957 1.824
    2501.358 1.431 1.395 1.262
    1250.812 0.832 0.822 0.689
    62.50.536 0.576 0.556 0.423
    31.20.342 0.356 0.349 0.216
    15.60.241 0.249 0.245 0.112
    00.132 0.134 0.133 　
  • 本试剂盒所含材料：
    • A micro ELISA plate --The 96-well plate has been pre-coated with an anti-human MUC5AC antibody.This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labled MUC5AC antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
    • One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
    • One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
    • An instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Gel-forming glycoprotein of gastric and respiratory tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microorganisms and particles that are subsequently removed by the mucociliary system. Interacts with H.pylori in the gastric epithelium, Barrett's esophagus as well as in gastric metaplasia of the duodenum (GMD).
  • 基因功能参考文献：
    1. S100A12 activates NLPR3 inflammasomes to induce MUC5AC production in airway epithelial cells. ATP induces MUC5AC production in a mechanistically similar mode to S100A12. PMID: 29906464
    2. The results of the present study indicate that Munc132 may be an essential regulator of basal MUC5AC exocytosis, while Munc134 appears to be a Munc13 protein subtype that may to be sensitive to hNE stimulation during airway MUC5AC hypersecretion. PMID: 29767240
    3. resistin induced MUC5AC and MUC5B expression via activation of different signaling pathways in human airway epithelial cells. PMID: 29604272
    4. the splice switch to DeltaC-ZIP2 as well as decreased expression of other ZIPs caused zinc deficiency, which is sufficient for induction of MUC5AC. PMID: 29289532
    5. this paper shows that IL-1beta upregulates Muc5ac expression via NF-kappaB-induced HIF-1alpha in asthma PMID: 29031476
    6. Data suggest that secreted IL-6 regulates CLB2.0-induced MUC5AC and MUC1 expression via ROS-mediated downregulation of claudin-1 expression to maintain mucus homeostasis in the airway. PMID: 28946937
    7. Helicobacter pylori (H. pylori)-CagA significantly upregulated MUC5AC, MUC2, and MUC5B expression in AGS cells, but did not affect E-cadherin and MUC6 expression. PMID: 29869461
    8. Oct-4 expression and the reduction of MUC5AC expression may be involved in the progression and an unfavorable prognosis of GC PMID: 28762513
    9. smoking might decrease tear secretion, goblet cell density and tear MUC5AC concentration. PMID: 27297822
    10. High-Mobility Group Box 1 Upregulates MUC5AC and MUC5B Expression in Primary Airway Epithelial Cells PMID: 29286856
    11. Cyclic pressure did not induce MUC5AC secretion in the airway mucus epithelium via Ca(2+)-dependent ATP release, and nearly all Ca(2+) was provided by stored intracellular Ca(2). PMID: 27919041
    12. IL-33 induced MUC5AC mRNA and MUC5AC protein, and also goblet cell hyperplasia at air liquid interface culture in human nasal epithelial cells. In addition to that, IL-33 induced MUC5B and FOXA3, and reduces FOXJmRNA. PMID: 27776277
    13. MUC5AC has pathologic roles in mechanisms of allergen-induced airway hyperresponsiveness, mucous metaplasia, and airway mucus plugging. PMID: 27845589
    14. Leptin knockdown suppressed MUC5AC production and secretion induced by IL-13 in human bronchial epithelial cells. PMID: 28942146
    15. The results suggest that the analysis of expression of MUC5AC and TFF1 may be useful for differentiating sessile serrated adenomas/polyps (SSA/Ps) from hyperplastic polyps (HPs). PMID: 28430953
    16. The Egr-1 is essential for CSE-induced MUC5AC production in HBE cells likely through interaction with and modulation of AP-1, and re-emphasize targeting Egr-1 as a novel therapeutic strategy for COPD. PMID: 28602698
    17. Mucus plugs from individuals with fatal asthma are heterogeneous gels with distinct MUC5AC- and MUC5B-containing domains. Tethering of MUC5AC-containing domains to the epithelium causes mucostasis and likely represents a major cause of mucus plugging in asthma. PMID: 27183390
    18. The only individual marker that was found to have direct and strong correlation with the clinical outcome of ampullary carcinoma was MUC5AC (not used in the Ang or Chang panels), with statistically significant survival differences found with various cutoffs tested (for 20% cutoff, 5-y survival, 68% vs. 31%; P=0.0002). In addition, MUC5AC significantly stratified the histologically pancreatobiliary and intestinal cases. PMID: 28505002
    19. MUC5AC interacts with integrin beta4 that mediates phosphorylation of FAK at Y397 leading to lung cancer cell migration. PMID: 26751774
    20. Results show that the glycoforms of MUC5AC in mucinous cysts of the pancreas display two unusual motifs, terminal alphaGlcNAc and terminal betaGlcNAc. PMID: 27992432
    21. MUC5AC expression was significantly higher in pancreatic adenocarcinoma than in healthy controls and chronic pancreatitis patients. PMID: 27845339
    22. Cigarette smoke exposure initially triggers the production of mitoROS and subsequently elicits autophagy in airway epithelial cells, which then regulate MUC5AC expression partially through modulation of the JNK signaling and the transcription factor AP-1. PMID: 27084849
    23. this study shows that S-allylmercapto-l-cysteine can suppress MUC5AC secretion using the cell model of chronic obstructive pulmonary disease PMID: 27517516
    24. This paper aims at developing a quantitative computer-aided methodology for automated identification of mucin areas and its percentage using tissue histological images PMID: 26971129
    25. MUC5B was significantly more often detected in middle ear effusion fluid relative to MUC5AC. MUC5AC presence was not significantly different in mucoid and serous fluid. PMID: 27729120
    26. outcome, while the gain of aberrant expression of MUC5AC and particularly of MUC6 was associated with favorable outcome in colorectal cancer, notably in intermediate stages II and III PMID: 27298226
    27. Low mucin1 expression is associated with peritoneal dissemination of gastrointestinal cancer. PMID: 27193208
    28. The HNE-TACE signalling pathway has an important role in the process of MUC5AC overexpression in chronic rhinosinusitis. PMID: 26881964
    29. Knockdown of MUC5AC in SW620 cells remarkably suppressed cell vitality and promoted apoptosis and G1 cell cycle arrest, resulting in the impaired ability of colony formation. The inhibition of MUC5AC in SW620 cells dramatically repressed the cell migration and invasion. PMID: 27610469
    30. These results suggest that IFN-gamma represses MUC5AC expression, disturbing binding of Sp1 to its target sequences. PMID: 27324793
    31. this study shows that SYK increased MUC5AC expression via ERK2 and p38 MAPK signaling pathways in airway epithelial cells PMID: 26980390
    32. The interference of p38 gene expression inhibited the release of IL-1beta and TNF-alpha in cultured cells. It also depressed both mRNA and protein levels of MUC5A. PMID: 26722604
    33. The expression of messenger RNAs is inhibited by EGFR tyrosine kinase inhibitor PMID: 26867523
    34. is a major component of the mucus produced by airway epithelial cells. PMID: 26100173
    35. Review-Meta-analysis: decreased Muc5AC expression might be a poor prognostic predictor for gastric cancer. PMID: 26420972
    36. Data show that verproside inhibits tumor necrosis factor alpha-induced mucin 5AC (MUC5AC) expression through NF-kappa B pathway. PMID: 26318254
    37. Overexpression of MUC5AC has been described in idiopathic pulmonary fibrosis lungs. Read More: http://www.atsjournals.org/doi/full/10.1164/rccm.201507-1322LE#.V2WAGNLrtNs PMID: 26871672
    38. The expression of ALPi and MUC5AC in cocultures of Caco-2 and HT29 cells developed for permeability studies is reported. PMID: 26299896
    39. MUC5AC hypomethylation is predictive of serrated lesions with malignant potential PMID: 26476272
    40. EGF removal or tyrphostin AG1478 treatment of differentiating air-liquid interface cultures from asthmatic children would result in a reduction of epithelial goblet cells and mucus secretion. PMID: 26057128
    41. These data lend support to the notion that beta2AR-agonists may contribute to asthma exacerbations by increasing mucin production via activation of beta2ARs on epithelial cells. PMID: 26161982
    42. The measurement of the CA 19-9 antigen on individual proteins could improve performance over the standard CA 19-9 assay. PMID: 22220206
    43. Studied the effects of 15-HETE on MUC5AC expression and related pathways. PMID: 25649981
    44. Increased expression of MUC5AC was associated with bacterial biofilm formation in chronic rhinosinusitis patients. PMID: 25638393
    45. Leukotriene E4 induces MUC5AC release from human airway epithelial NCI-H292 cells PMID: 25838093
    46. Results showed that MUC5AC expression is correlated to the tumor stage where advanced gastric cancers present reduced levels of MUC5AC and that Gli regulates its expression via interaction with a highly conserved sequence in its promoter region. PMID: 25166306
    47. MUC5AC expression in ovarian mucinous tumors did not change from benign to malignant tumors. PMID: 25298197
    48. Common genetic variations in the MUC5AC gene are not related to helicobacter pylori serologic status. PMID: 25605164
    49. The genetic length variants in the central repetitive region of MUC5AC were not associated with meconium ileus in cystic fibrosis. PMID: 24920497
    50. Data suggest that determination of mucin 5AC (MUC5AC) methylation status may be useful for understanding and predicting the natural history of colorectal cancers (CRC). PMID: 25403854

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  • 亚细胞定位：
    Secreted.
  • 组织特异性：
    Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum.
  • 数据库链接：

    HGNC: 7515

    OMIM: 158373

    KEGG: hsa:4586

    STRING: 9606.ENSP00000435591

    UniGene: Hs.534332