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Human melanin ELISA kit

  • 中文名称：
    人黑色素酶联免疫试剂盒
  • 货号：
    CSB-E14051h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human melanin ELISA Kit was designed for the quantitative measurement of Human melanin protein in serum. It is a Competitive ELISA kit, its detection range is 12.5 pg/mL-1000 pg/mL and the sensitivity is 15.6 pg/mL.

  • 缩写：
    melanin
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum
  • 检测范围：
    12.5 pg/mL-1000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human melanin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 89-102  
    1:2 Average % 95  
    Range % 91-100  
    1:4 Average % 94  
    Range % 92-97  
    1:8 Average % 95  
    Range % 90-101  
  • 回收率：
    The recovery of human melanin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 92-105  
    EDTA plasma (n=4) 96 91-103  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average 　  
    1000 0.145 0.147 0.146 　  
    375 0.276 0.286 0.281 　  
    187.5 0.402 0.413 0.408 　  
    62.5 0.553 0.562 0.558 　  
    12.5 1.051 1.084 1.068 　  
    0 2.453 2.567 2.510 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Which type of melanin do you dose?The three types have different molecular structures. how can we be sure that the antibodies recognize all the target? And so my question is how can we be sure that we dose the whole melanin? Thanks for your help!

A:
Thanks for your inquiry. Our kit detects the general melanin and has no detailed types. Small molecules are also different in vivo and in vitro. The body may be combined with other proteins or small molecules to aggregate, and our box detects such a total form. In addition, the small molecule is the reference https://pubchem.ncbi.nlm.nih.gov/compound/6325610#section=Top design.Pls let me know if you have any further questions. Thank you.