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  4. Human low-density lipoprotein-receptor-related protein 1,LRP-1 ELISA Kit

Human low-density lipoprotein-receptor-related protein 1,LRP-1 ELISA Kit

  • 中文名称：
    人低密度脂蛋白受体相关蛋白1(LRP-1)酶联免疫试剂盒
  • 货号：
    CSB-E13348h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This human LRP1 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human LRP1 in multiple samples, including serum, plasma, cell lysates, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the LRP1 levels in the sample and is measured at 450 nm through a microplate reader.

    LRP1 is involved in two major physiological processes: endocytosis and regulation of signaling pathways, which play an important role in multiple biological activities, including regulation of cell growth and differentiation, modulation of cytoskeletal organization angiogenesis, cell migration, and regulation of protease degradation for tissue invasion. LRP1 mediates the endocytosis of a diverse set of extracellular ligands that play important roles in tumor progression. Additionally, LRP1 can initiate and regulate diverse signaling pathways, including MAPK, insulin receptor (IR), serine/AKT, ERK, and JNK pathways. LRP1 has been postulated to participate in numerous diverse physiological and pathological processes ranging from plasma lipoprotein homeostasis, atherosclerosis, tumor evolution, and fibrinolysis to neuronal regeneration and survival.

  • 别名：
    A2MR ELISA Kit; Alpha 2 macroglobulin receptor ELISA Kit; alpha 2MR ELISA Kit; Alpha-2-macroglobulin receptor ELISA Kit; APOER ELISA Kit; Apolipoprotein E receptor ELISA Kit; APR ELISA Kit; CD 91 ELISA Kit; CD91 ELISA Kit; CD91 antigen ELISA Kit; IGFBP3R ELISA Kit; LDL receptor related protein 1 ELISA Kit; Low density lipoprotein receptor related protein 1 ELISA Kit; Low density lipoprotein related protein 1 ELISA Kit; Low-density lipoprotein receptor-related protein 1 intracellular domain ELISA Kit; LRP 1 ELISA Kit; LRP 515 ELISA Kit; LRP 85 ELISA Kit; LRP ELISA Kit; LRP ICD ELISA Kit; LRP-1 ELISA Kit; LRP-515 ELISA Kit; LRP-85 ELISA Kit; Lrp1 ELISA Kit; LRP1 protein ELISA Kit; LRP1_HUMAN ELISA Kit; LRP1A ELISA Kit; LRP515 ELISA Kit; LRP85 ELISA Kit; LRPICD ELISA Kit; MGC88725 ELISA Kit; Prolow density lipoprotein receptor related protein 1 ELISA Kit; TbetaR V/LRP 1/IGFBP 3 receptor ELISA Kit; TbetaRV/LRP1/IGFBP3 receptor ELISA Kit; TGFBR 5 ELISA Kit; TGFBR5 ELISA Kit; Type V tgf beta receptor ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.625 ng/mL-40 ng/mL
  • 灵敏度：
    0.156 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human LRP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %93
    Range %88-97
    1:2Average %84
    Range %80-89
    1:4Average %102
    Range %96-108
    1:8Average %97
    Range %91-102
  • 回收率：
    The recovery of human LRP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8682-94
    EDTA plasma (n=4)9992-107
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    402.455 2.354 2.405 2.262
    201.628 1.527 1.578 1.435
    100.976 0.955 0.966 0.823
    50.566 0.577 0.572 0.429
    2.50.371 0.382 0.377 0.234
    1.250.267 0.278 0.273 0.130
    0.6250.210 0.216 0.213 0.070
    00.142 0.143 0.143 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. Required for early embryonic development. Involved in cellular lipid homeostasis. Involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. Acts as an LRPAP1 alpha-2-macroglobulin receptor. Acts as TAU/MAPT receptor and controls the endocytosis of TAU/MAPT as well as its subsequent spread. May modulate cellular events, such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling as well as neurotransmission.; (Microbial infection) Functions as a receptor for Pseudomonas aeruginosa exotoxin A.
  • 基因功能参考文献：
    1. Association of low-density lipoprotein receptor-related protein 1 rs11613352 and angiopoietin-like 3 rs2131925 with hypertension might imply a direct effect at the artery wall. PMID: 29989339
    2. The results presented in this study raise the hypothesis that higher production of LRP1 protein may be associated with higher endocytosis of upregulated transporter proteins at the cell surface, and hence, increased dox and emodin accumulation and growth inhibition. If so, elevation of LRP1 expression may be a useful target for interventions to promote the efficacy of these and other anticancer drugs. PMID: 29644529
    3. This study is the first to determine that the sLR11 gene rs3824968 polymorphic of factor T may increase the risk of carotid atherosclerosis in Type 2 Diabetes Mellitus patients by regulating the concentration of LDL-C in Korean and Han nationalities in China PMID: 29865095
    4. A common polymorphism decreases LRP1 mRNA stability and is associated with increased plasma factor VIII levels. PMID: 28431990
    5. circulating sLRP1 is a novel surrogate biomarker of EAT volume in T1DM patients. PMID: 29348672
    6. Data show that amyloid precursor protein (APP) dimerization affects its interaction with LDL receptor related protein 1 (LRP1) and LDL-receptor related protein SorLA (SorLA), suggesting that APP dimerization modulates its interplay with sorting molecules and in turn its localization and processing. PMID: 28799085
    7. LRP1 mediates cell adhesion by orchestrating a multi-protein pathway to activate, traffic and degrade integrins. Thus, LRP1 may serve as a focal point in the integrin quality control system to ensure a firm connection to the extracellular matrix. PMID: 29116364
    8. This study defines LRP1 as a regulator of CXCR3, which may have important consequences for tumor biology. PMID: 29146996
    9. The C4408R mutant located at the APP695 alpha-secretase cleavage site of LRP1, when expressed in CHO cells expressing APPswe or wild-type APP (APPwt), co-expression of LRP1-CT C4408R decreases Abeta and increases sAPPalpha and alpha-CTF compared with co-expression of wild-type LRP1-CT. LRP1-CT C4408R enhanced the unglycosylated form of LRP1-CT and reduced APP endocytosis. PMID: 28612181
    10. In cerebral blood vessels, LRP1 is an important mediator for the rapid removal of ABETA from brain via transport across the blood-brain barrier. This work summarizes recent findings on LRP1 function and discuss the targeting of LRP1 as a modulator for Alzheimer's Disease pathology and drug delivery into the brain. [review] PMID: 28948494
    11. Extracellular vesicles do not contribute to higher circulating levels of soluble LRP1 in idiopathic dilated cardiomyopathy. PMID: 28557183
    12. LRP-1 silencing leads to a decrease of cell migratory capacity in a 3D configuration. PMID: 27463962
    13. LRP1 has a role in insulin signaling and is a potential role as a link between lipoprotein and glucose metabolism in diabetes [review] PMID: 28584820
    14. Results indicate that holo-Lf, but not apo-Lf, increases TE expression through LRP-1 in human dermal fibroblasts and suggest that holo-Lf and TGF-beta1 enhance TE expression by activating the PI3K/Akt1 and PI3K/Akt2 pathways, respectively. PMID: 28833753
    15. Development of a monoclonal anti-ADAMTS-5 antibody that specifically blocks the interaction with LRP1. PMID: 28306378
    16. MMP-13 may play a role on physiological turnover of cartilage extracellular matrix and that LRP1 is a key modulator of extracellular levels of MMP-13 and its internalization is independent of the levels of ADAMTS-4, -5 and TIMP-3. PMID: 27084377
    17. Dissecting the interaction between TIMP3 and LRP1 using a synthetic analog of the LRP1 receptor has been reported. PMID: 27476612
    18. FVIIa-antithrombin but not FVIIa is a ligand for LRP1, and LRP1 contributes to the clearance of FVIIa-antithrombin in vivo PMID: 27614059
    19. Activated alpha2 -Macroglobulin Induces Mesenchymal Cellular Migration Of Raw264.7 Cells Through Low-Density Lipoprotein Receptor-Related Protein 1 PMID: 28012205
    20. Study demonstrated that LRP1 expression is significantly upregulated by myeloid cells in active multiple sclerosis lesions in comparison to the surrounding healthy tissue. Results suggest that the function of LRP1 in microglia is to keep these cells in an anti-inflammatory and neuroprotective status during inflammatory insult. PMID: 27400748
    21. Poor LRP1 expression in T cells depends on shedding. Integrin ligands and CXCL12 antagonize shedding through a TSP-1-dependent pathway and ligation of CD28 antagonizes shedding independent of TSP-1. PMID: 28580688
    22. Altered Met receptor phosphorylation and LRP1-mediated uptake in cells lacking carbohydrate-dependent lysosomal targeting PMID: 28724630
    23. LRP1 single-nucleotide polymorphism is associated with migraine. PMID: 27322543
    24. Electrostatic potential calculations suggested a competition between negatively charged GAGs and highly negatively charged complement-like domains of LRP-1 for the binding to a positively charged area of TIMP-3 as an underlying mechanism. PMID: 27610455
    25. findings revealed a pH-dependent release of the ligand associated with a conformational change of the receptor. In summary, this investigation of the complete LRP1 ectodomain significantly advances our understanding of this important receptor and provides the basis for further elucidating the mechanism of action of LRP1 in a whole and integrated system. PMID: 27956551
    26. although fVIII bound avidly to soluble forms of clusters II and IV from LRP1, only soluble cluster IV competed with the binding of fVIII to full-length LRP1, revealing that cluster IV represents the major fVIII binding site in LRP1. PMID: 27794518
    27. LRP1 protein expression in human abdominal aortic aneurysm tissues may be down-regulated by miR-205 through translational inhibition, resulting in a reduced clearance of pericellular MMP-9, promoting aneurysm formation. PMID: 26781079
    28. data also reveal that D1D2 is able to bind to a second distinct site on LRP1 to form a monovalent complex. The studies confirm the canonical model for ligand recognition by this class of receptors, which is initiated by pairs of lysine residues that dock into acidic pockets on the receptor PMID: 27402839
    29. results indicate that genetic variations in LRP1 and ULK4 contribute to risk for presenting with an acute aortic dissection PMID: 27569546
    30. Considering that LRP-1, by mediating the clearance of matrix metalloproteinases, is involved in the regulation of extracellular matrix remodeling and cell migration, we conclude that a decreased expression of LRP-1 could be involved with the increasing activity of MMPs shown in cancers. PMID: 27931798
    31. These data suggest that the Rho/ROCK signaling pathway is involved in HIV-1 Tat-mediated changes in occludin, RAGE, and LRP1 in human cerebral microvascular endothelial cells cells. PMID: 27563375
    32. Studies demonstrate a role for ADAM10 in the ectodomain shedding of LRP1 in the brain and the clearance of Abeta across the blood-brain barrier, which may provide a novel strategy for attenuating Abeta accumulation in the AD brain PMID: 27503326
    33. Results provide evidence that TTR acts as a carrier of Abeta at the blood-brain-barrier and liver, using LRP1. PMID: 26837706
    34. Studies show the ability of LRP1 to promote endocytosis and deliver cell signaling suggesting multiple roles in tumorigenesis and tumor progression. In addition, LRP1 has dual effects on tumor cell invasion and migration. Moreover, LRP1 can be regulated via methylation of its CpG islands. [review] PMID: 26738504
    35. Study demonstrated that 1,25(OH)2D3, the active form of vitamin D, plays an important role in increasing Abeta1-40 vectorial transport from the brain to blood and systemic clearance from peripheral circulation through increasing LRP1 levels both in vivo and in vitro, and reducing RAGE level in the blood-brain barrier model in vitro PMID: 26820600
    36. Circulating soluble LRP1 concentrations were associated with the occurrence of carotid atherosclerosis in a hypercholesterolemia population. PMID: 26285183
    37. LRP1 as a pathogenic gene for autosomal recessive Keratosis pilaris atrophicans and keratosis pilaris PMID: 26142438
    38. Suggest role for LRP1/PARP1 signaling in endothelial cell proliferation and retinal neovascularization induced by hypoxia. PMID: 26634655
    39. it was suggested that a cell surface-expressed beta-galactoside binding protein, galectin-8, was involved in factor V endocytosis. PMID: 25800007
    40. alpha1-antitrypsin internalization is a clathrin-dependent and low-density lipoprotein receptor-related protein 1-mediated endocytosis process. PMID: 26206901
    41. LRP1, p38 MAPK and ERK1/2 mediate the internalization of beta-amyloid 1-42 in neurons. PMID: 25936756
    42. conclusion, C667T polymorphism of LRP-1 is moderately but significantly associated with global and regional amyloid deposition in AD. PMID: 24596678
    43. LRP1 variants may be associated with risk of ischaemic stroke. PMID: 26031789
    44. Lrp1-antisense directly binds to high-mobility group box 2 (Hmgb2) and inhibits the activity of Hmgb2 to enhance Srebp1a-dependent transcription of Lrp1. PMID: 25937287
    45. Statistically significant associations were also found for the PS1 1/2 polymorphism in both the dominant and recessive genetic models, whereas no association was found for the LRP C/T polymorphism PMID: 25730041
    46. Data suggest that aortic but not circulating LRP1 is downregulated in patients with AAA and indicates a possible role for this protein in clearing an aneurysm-relevant ligand. PMID: 26188720
    47. Two recurrent fusion genes associated with the 12q locus, LRP1-SNRNP25 and KCNMB4-CCND3, were by RT-PCR, Sanger sequencing and FISH, and were found to be osteosarcoma specific in a validation cohort of 240 other sarcomas. PMID: 25300797
    48. The interaction between factor VIII and LRP1 occurs over an extended surface containing multiple lysine residues. PMID: 25903134
    49. domain CR9 appears to be critical for LRP1-mediated AgLDL binding and internalization in hVSMCs. Our results open new avenues for an innovative anti-VSMC foam cell-based strategy for the treatment of vascular lipid deposition in atherosclerosis PMID: 25918169
    50. rs1466535 LRP1 polymorphism is not a significant and independent risk factor for carotid artery stenosis PMID: 25238221

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  • 相关疾病：
    Keratosis pilaris atrophicans (KPA)
  • 亚细胞定位：
    [Low-density lipoprotein receptor-related protein 1 85 kDa subunit]: Cell membrane; Single-pass type I membrane protein. Membrane, coated pit.; [Low-density lipoprotein receptor-related protein 1 515 kDa subunit]: Cell membrane; Peripheral membrane protein; Extracellular side. Membrane, coated pit.; [Low-density lipoprotein receptor-related protein 1 intracellular domain]: Cytoplasm. Nucleus.; Golgi outpost. Cytoplasm, cytoskeleton, microtubule organizing center.
  • 蛋白家族：
    LDLR family
  • 组织特异性：
    Most abundant in liver, brain and lung.
  • 数据库链接：

    HGNC: 6692

    OMIM: 107770

    KEGG: hsa:4035

    STRING: 9606.ENSP00000243077

    UniGene: Hs.162757