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  4. Human intestinal fatty acid binding protein,iFABP ELISA Kit

Human intestinal fatty acid binding protein,iFABP ELISA Kit

  • 中文名称：
    人肠脂肪酸结合蛋白(iFABP)酶联免疫试剂盒
  • 货号：
    CSB-E08024h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：
        肠型脂肪酸结合蛋白2（Fatty Acid Binding Protein 2），是一种细胞质内蛋白质，在小肠、肝脏、心肌等组织中表达。它可以结合并转运脂肪酸和其他疏水性物质，调节脂质代谢。FABP2在肥胖、糖尿病等疾病的发生和发展中扮演着重要角色。
        j9九游会登录入口首页生物所提供的Human intestinal fatty acid binding protein,iFABP ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的FABP2，其灵敏度为0.039 ng/mL ，检测范围为0.156 ng/mL-10 ng/mL。
  • 别名：
    FABP 2 ELISA Kit; FABP2 ELISA Kit; FABPI ELISA Kit; FABPI_HUMAN ELISA Kit; Fatty acid binding protein 2 intestinal ELISA Kit; Fatty acid binding protein ELISA Kit; Fatty acid binding protein intestinal ELISA Kit; Fatty acid-binding protein 2 ELISA Kit; Fatty acid-binding protein ELISA Kit; I FABP ELISA Kit; I-FABP ELISA Kit; IFABP ELISA Kit; intestinal ELISA Kit; intestinal FABP ELISA Kit; Intestinal fatty acid binding protein 2 ELISA Kit; Intestinal-type fatty acid-binding protein ELISA Kit; MGC133132 ELISA Kit; OTTHUMP00000163925 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 本试剂盒所含材料：
      • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human FABP2 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled FABP2 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 最新研究进展：
    FABP2，全称脂肪酸结合蛋白2（Fatty Acid-Binding Protein 2），也称为肠型脂肪酸结合蛋白（I-FABP），是一种小分子（约15kDa）的细胞内蛋白。FABP2主要在肠道内吸收和代谢脂肪酸时发挥作用，它可以在肠道细胞内和血液中很快地转运脂肪酸，并通过运输脂肪酸的过程参与脂质代谢和细胞能量代谢。FABP2在心血管疾病、代谢综合征、炎症和癌症等多种疾病的发生和发展中具有重要作用。最新研究表明，FABP2可能与非酒精性脂肪性肝病（NAFLD）的发生和发展有关。一项研究发现，FABP2基因敲除小鼠的脂肪酸吸收和氧化能力降低，这种转化过程紊乱使得机体易于出现NAFLD。此外，一些临床研究也发现，FABP2与NAFLD的发生和严重程度呈正相关。
  • 功能：
    FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
  • 基因功能参考文献：
    1. Higher intestinal fatty acid binding protein (i-FABP) levels on admission were associated with pancreatic necrosis, systemic complications, and severe acute pancreatitis (AP). PMID: 29771764
    2. serum level in newborns positively correlated with second stage of labor duration and active pushing time PMID: 28110600
    3. We found that polymorphic Arg16Gly in the ADRB2 gene and Ala54Thr in the FABP2 gene had a discernible effect on the renal function in Chinese type 2 DN. Our data further revealed that homozygous Gly16 allele and Thr54 allele concomitantly conferred a significantly higher risk in the eGFR reduction. PMID: 29404926
    4. This study aimed to determine whether FABP2 (Ala54Thr) and MTTP (-493 G/T) genetic polymorphisms are associated with metabolic disorders in Mexican subjects. PMID: 29338565
    5. Data show that intestinal fatty acid binding protein (IFABP) is a promising prognostic marker in acute pancreatitis. PMID: 28984785
    6. There was a positive correlation between FABP2 levels and BMI, SBP and DBP, and a negative correlation with HDL-C. CONCLUSIONS: The Thr54 allele of the FABP2 Ala54Thr polymorphism was associated with an increased incidence of peripheral atherosclerosis combined with T2DM in the population studied PMID: 27778448
    7. The FABP-2 gene is located on the long arm of chromosome 4, and encodes an intracellular protein of the intestinal mucosa, responsible for the absorption and intracellular transport of fatty acids. PMID: 28760427
    8. Enterocyte injury was common in critically ill patients with pneumonia. The severity of enterocyte injury, as evidenced by the urinary FABP2/creatinine, was associated with the patient's mortality. PMID: 28164671
    9. Study reports that insulin resistance-related gene polymorphisms effects colorectal cancer (CRC) risk. The results showed that the gene polymorphism of ADIPOQ rs2241766 was associated with CRC risk. Furthermore, the interactions of ADIPOQ rs2241766, UCP2 rs659366, FABP2 rs1799883 and red meat consumption may contribute to the risk of CRC. PMID: 23826253
    10. FTO and FABP2 gene polymorphisms were significantly associated with susceptibility to metabolic syndrome and obesity in this cohort. PMID: 28738793
    11. Circulating I-FABP levels had no association with invasively-measured hemodynamic parameters, but were associated with adverse clinical outcomes in patients with acute decompensated heart failure with systolic dysfunction. PMID: 28232029
    12. The aim of this study was to measure serum I-FABP levels and provide the transition of I-FABP levels with hypothermic circulatory arrest to help in the management of intestinal perfusion. Plasma I-FABP monitoring could be a valuable method for finding an intestinal ischemia in patients with cardiovascular surgery. PMID: 27765895
    13. Urinary intestinal fatty acid-binding protein can distinguish necrotizing enterocolitis from sepsis in early stage of the disease. PMID: 27110575
    14. I-FABP is a valid serologic biomarker for early diagnosis in NEC for the premature neonates with a moderate accuracy. PMID: 26798632
    15. It is concluded that ACE (rs 4646994), FABP2 (rs1799883) and GST (GSTM1 null or positive genotype and GSTT1 null or positive genotype) genes polymorphism are associated with essential hypertension. PMID: 25357227
    16. Ala54Thr polymorphism of FABP2 modulates HDL cholesterol in Mexican-Americans with type 2 diabetes. PMID: 26703680
    17. Carriers of the Thr54 allele in FABP2 have a different metabolic response after weight loss than wild type non-A obese carriers PMID: 26316042
    18. Letter: serum intestinal-fatty acid binding protein may serve as a biomarker for refractory celiac disease. PMID: 26114190
    19. Ala54Thr polymorphism of FABP2 was associated with high triglycerides levels, but not to gallstone disease. PMID: 26019038
    20. FABP2 Ala54Thr polymorphism may have a role in type 2 diabetes, obesity, and metabolic syndrome [case-control study and meta-analysis] PMID: 25730055
    21. Both plasma and urinary I-FABP levels specifically identify necrotizing enterocolitis in preterm infants. PMID: 25793701
    22. FABP2 is a gene candidate for predisposition to Diabetic nephropathy, as it has been linked to microalbuminuria in patients with Type 2 Diabetes mellitus. PMID: 25594612
    23. To determine the allele/genotype frequencies of Ala54Thr polymorphism of the FABP2 gene. PMID: 26238699
    24. Increasing gestational age is accompanied by an increase in ileal I-FABP tissue content. PMID: 24956227
    25. Studies suggest that the fatty acid binding protein 2 (FABP2) Ala54Thr polymorphisms (rs1799883) are associated with increased susceptibility to type 2 diabetes mellitus risk (T2DM) risk among Asians but not Caucasians. PMID: 25388378
    26. ACE (I/D) and FABP2 (Ala54Thr) genes polymorphism and its susceptibility to type 2 diabetes mellitus in the North Indian population, was studied. PMID: 23468166
    27. Ala54Thr of FABP2 may be not associated with stroke risk but associated with plasma triglyceride level of stroke patients for Hunan Han population of China. PMID: 25262933
    28. systemic marker for Crohn disease activity PMID: 25807642
    29. The study suggests that the above named variant in FABP2 gene is not potential contributor to the risk of T2DM and related traits in a Saudi population. PMID: 24690233
    30. Systemic I-FABP levels appear valuable in detecting irreversible intestinal ischemia-reperfusion damage. PMID: 24100750
    31. the Ala54Thr fatty acid-binding protein 2 (FABP2) polymorphism may have a role in recurrent depression and is associated with fatty acid concentrations and waist circumference PMID: 24340071
    32. FABP2 Thr54 carriers have a better metabolic response than obese carriers with Ala54Ala genotype to high-polyunsaturated dietary fats. PMID: 23072901
    33. FABP2 gene Ala54Thr polymorphism is associated with the predisposition to physical endurance and is not associated with risk of obesity and body fat mass. PMID: 24640155
    34. INSR rs1366600, ACSL1 rs2292899 and FABP2 rs11724758 could influence the susceptibility to type 2 diabetes in Chinese Han population, most likely through their effects on the specific miRNA-binding sites. PMID: 23303383
    35. Carriers of Thr54 allele of FABP2 have a different response than wild type obese, with a lack of decrease of insulin levels, leptin levels and HOMA-Insulin resistance. PMID: 23817228
    36. Polymorphisms in FABP2 and APOL6, located at the predicted miRNAs binding sites, are identified to contribute to susceptibility to MetS in the Chinese Han population. PMID: 23911300
    37. results suggest that the higher cardiorespiratory fitness may attenuate the changes in central arterial stiffness and blood pressure that are associated with the FABP2 genotype PMID: 23362142
    38. Thr54 allele carriers responded better to a moderate-fat diet in overweight or obese subjects. PMID: 22817827
    39. Mutation frequency of FABP2 gene in the people with dyslipidemia was significantly higher than that in the people with normal blood lipids. PMID: 21861348
    40. FABP2 genetic polymorphism is associated with lower triglyceride and higher HDL-cholesterol concentrations in elderly subjects with MetS PMID: 22838187
    41. The Ala54Thr polymorphism in the FABP2 gene was not associated with obesity in the Chinese Han population of the Chengdu area. PMID: 21355293
    42. meta-analysis does not support the association between the FABP2 Ala54Thr polymorphism and body mass index PMID: 20621703
    43. We hypothesized that the triglyceride-rich lipoproteins response to diets of varied fat content is affected by the FABP2 A54T polymorphism....no evidence of an interaction, which does not support our hypothesis. PMID: 21840466
    44. The findings suggest a potential distinct role(s) of SNPs in FABP5 and FABP2 genes in T2DM in different populations. PMID: 21288588
    45. The finding of ths study was the lack of association of the Thr54/Ala54 and Thr54/Thr54 fatty acid binding protein 2 genotypes with metabolic syndrome. PMID: 20723947
    46. genetic association studies [Iran]: FABP2 SNP affects response to eicosapentaenoic acid supplementation in hypertriglyceridemia; more favorable effects on blood lipids/lipoproteins in subjects with Ala54/Thr54 genotype than with Ala54/Ala54 genotype PMID: 20080390
    47. FABP2 Thr54Thr genotype might increase the risk of non-alcoholic fatty liver disease. PMID: 19689066
    48. Thr54 allele of the FABP2 Ala54Thr is weakly associated with a higher degree of insulin resistance, higher level of fasting insulin and higher level of 2-h blood glucose. PMID: 20578207
    49. TT genotype in Ala54Thr polymorphism of FABP2 gene in patients with type 2 diabetes increased dietary fatty acid absorption, and this might increase the susceptibility to the effects of dietary lipids. PMID: 20484485
    50. data indicate that intestinal fatty acid binding protein (FABP2) expression depends on (n-3) fatty acid intake and FABP2 genotypes PMID: 20534879

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Calycin superfamily, Fatty-acid binding protein (FABP) family
  • 组织特异性：
    Expressed in the small intestine and at much lower levels in the large intestine. Highest expression levels in the jejunum.
  • 数据库链接：

    HGNC: 3556

    OMIM: 134640

    KEGG: hsa:2169

    STRING: 9606.ENSP00000274024

    UniGene: Hs.282265