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  4. Human interferon-inducible protein 10,IP-10 ELISA Kit

Human interferon-inducible protein 10,IP-10 ELISA Kit

  • 中文名称：
    人干扰素诱导蛋白10(IP-10/CXCL10)酶联免疫试剂盒
  • 货号：
    CSB-E08181h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：
        CXCL10是一种趋化因子，可以与其受体CXCR3相互作用。CXCL10主要由细胞因子和白细胞分泌，并在多种组织中发现，如淋巴结、肝和肺等。CXCL10参与了多种生物学过程，如炎症、免疫反应和肿瘤免疫等方面的作用。
        j9九游会登录入口首页生物所提供的Human interferon-inducible protein 10,IP-10 ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的CXCL10，其灵敏度为7.8 pg/mL，检测范围为31.25 pg/mL-2000 pg/mL。
     
  • 别名：
    Interferon gamma induced factor MOB1; mouse; homolog of ELISA Kit; Interferon gamma induced protein 10 ELISA Kit; 10 kDa interferon gamma induced protein ELISA Kit; 10 kDa interferon gamma-induced protein ELISA Kit; C X C motif chemokine 10 ELISA Kit; C7 ELISA Kit; Chemokine (C X C motif) ligand 10 ELISA Kit; Chemokine CXC motif ligand 10 ELISA Kit; Crg 2 ELISA Kit; CRG2 ELISA Kit; CXCL10 ELISA Kit; CXCL10(1-73) ELISA Kit; CXL10_HUMAN ELISA Kit; Gamma IP10 ELISA Kit; Gamma-IP10 ELISA Kit; gIP 10 ELISA Kit; GIP10 ELISA Kit; IFI10 ELISA Kit; INP 10 ELISA Kit; INP10 ELISA Kit; Interferon activated gene 10 ELISA Kit; Interferon activated gene 10 ELISA Kit; Interferon gamma induced cell line ELISA Kit; Interferon inducible cytokine IP 10 ELISA Kit; Interferon inducible cytokine IP10 ELISA Kit; IP 10 ELISA Kit; IP-10 ELISA Kit; Mob 1 ELISA Kit; MOB1 ELISA Kit; Protein 10 from interferon (gamma) induced cell line ELISA Kit; SCYB10 ELISA Kit; Small inducible cytokine B10 ELISA Kit; Small inducible cytokine B10 precursor ELISA Kit; Small inducible cytokine subfamily B (Cys X Cys) member 10 ELISA Kit; Small inducible cytokine subfamily B CXC member 10 ELISA Kit; Small inducible cytokine subfamily B; member 10 ELISA Kit; Small-inducible cytokine B10 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human IP-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 90  
    Range % 85-96  
    1:2 Average % 98  
    Range % 91-105  
    1:4 Average % 96  
    Range % 92-103  
    1:8 Average % 92  
    Range % 84-98  
  • 回收率：
    The recovery of human IP-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 88-96  
    EDTA plasma (n=4) 93 87-99  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2000 2.923 2.963 2.943 2.832  
    1000 2.151 2.176 2.164 2.053  
    500 1.255 1.276 1.266 1.155  
    250 0.706 0.671 0.689 0.578  
    125 0.434 0.399 0.417 0.306  
    62.5 0.299 0.285 0.292 0.181  
    31.25 0.248 0.253 0.251 0.140  
    0 0.110 0.111 0.111 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

平均分:
5.0分 - 1 个评价

样品类型：血浆（抗凝剂） IP-10

样品信息：人

稀释比：没有稀释

产品评价： 我们实验室绝大多数试剂盒都来自j9九游会登录入口首页生物CUSABIO，发货速度快，性能稳定，使用CSB-E08181h分别检测了实验和对照个体血浆中的干扰素-γ-诱导蛋白 10 (IP-10) 的水平，结果显示在实验个体中IP-10的含量有所增加，与预期的结果一致，能较好地完成实验计划，节省实验时间。而且价格也比较实惠，同一个课题组使用的不少，采购也方便。

By 王老师

靶点详情

  • 最新研究进展：
    CXCL10，也称为干扰素γ诱导蛋白-10（IP-10），是一种细胞因子，参与炎症和免疫反应。最新研究表明CXCL10在肿瘤免疫治疗中具有重要作用，它可以增强肿瘤细胞的免疫原性，并吸引和激活具有杀伤作用的T细胞。
  • 功能：
    Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects. Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites. Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization. In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation. Activation of the CXCL10/CXCR3 axis plays also an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization.
  • 基因功能参考文献：
    1. results of study suggest that the CXCL10 overexpression in the salivary glands is caused mainly by IFN-gamma-stimulated salivary gland ductal cells. PMID: 29549479
    2. Urinary CXCL10 reflects subclinical inflammation within the tubulointerstitial and peritubular capillary spaces, but not the vascular/systemic compartments PMID: 28902772
    3. CXCL10 in the serum of patients with vitiligo and alopecia areata was significantly elevated compared with healthy controls, but was similar between patients with vitiligo and AA PMID: 27863059
    4. IP-10 might be involved in the pathogenesis of thrombocytopenia (T), anasarca (A), fever (F), reticulin fibrosis (R), and organomegaly (O) -idiopathic Multicentric Castleman disease. PMID: 28205564
    5. CXCL10, not CXCL9 or CXCL11, induced IL-9 expression in the liver tissue. PMID: 29860220
    6. serum IP-10 level and the IFN-gamma/IL-4 ratio have great potential to predict significant fibrosis among chronic hepatitis B patients. PMID: 28067328
    7. No association between IP-10 serum-levels and cancer-related fatigue. PMID: 30188739
    8. CXCL10 rs1439490 G/G is positively associated with seronegative occult hepatitis C virus infection and antiviral treatment outcome. PMID: 29853737
    9. Age-related epigenetic and genetic factors which contribute to the dysregulation of CXCL10. PMID: 29223680
    10. COMMD7 activates CXCL10 production by regulating NFkappaB and the production of ROS. The present study highlighted the role of COMMD7 in the development of HCC, and provides novel options for anticancer drug design. PMID: 29532873
    11. Nab-PTX treatment could increase CXCL10 expression. PMID: 29902349
    12. The frequency of non-classical monocytes spontaneously producing CXCL10 was increased in both limited and diffuse systemic sclerosis PMID: 29127442
    13. serum levels in active vitiligo significantly elevated compared to those in stable vitiligo patients PMID: 29115683
    14. Plasma CXCL10 levels are significantly higher in SPs pre-HAART and RPs pre-HAART when compared with HIV-negative controls. PMID: 29122683
    15. Hepatitis b vaccine was efficient in enhancing IP-10 level with HBsAg clearance, or reduction to a favorable level. PMID: 28802168
    16. Results show that the expression of IP-10 in peripheral blood of patients with HBV-associated acute-on-chronic liver failure (HBV-ACLF) was significantly high and correlated with the severity of liver failure. IP-10 played an important role in the pathogenesis and progression of HBV-ACLF. PMID: 29058291
    17. Differential regulation of cytokine release at both transcriptional and post-transcriptional levels, suppresses type-I-IFN induction yet allows for CXCL10 secretion during imDNA-induced cellular stress. PMID: 27941826
    18. the elevated concentrations of CXCL13, CXCL8, and CXCL10 or their increasing CSF/serum ratios may be potential biomarkers of neurosyphilis PMID: 27650493
    19. Results highlight the role of phytohaemagglutin-stimulated peripheral blood mononuclear cells from patients with Alzheimer's disease on the expression of chemokines CXCL10 and CCL4 by endothelial cells and H4 cell line (mimicking the brain parenchyma) in a human blood brain barrier model. PMID: 28413983
    20. viral and bacterial co-infection modulates the JAK-STAT signaling pathway and leads to exacerbated IP-10 expression, which could play a major role in the pathogenesis of pneumonia. PMID: 27922126
    21. Study suggests that rs56061981 and rs56216945 in CXCL10 gene promoter contribute COPD susceptibility. PMID: 29285564
    22. Our findings showed that the assessment of serum IP-10 level could be a predictive factor for SVR and it was associated with fibrosis, necroinflammatory activity, significant steatosis and IR in patients with chronic HCV infection. PMID: 28862188
    23. In conclusion, IL-29 enhanced CXCL10 production in human oral epithelial cells via the p38 MAPK, STAT3, and NF-kappaB pathways, which might control Th1-cell accumulation in periodontal lesions and be involved in pathological processes in periodontal disease. PMID: 28753407
    24. Interferon-gamma-inducible protein 10 (IP-10) directly promoted hepatocyte apoptosis, and baseline IP-10 levels may predict the decrease in the hepatitis B surface antigen levels after entecavir therapy in patients with chronic hepatitis B. PMID: 28614914
    25. data indicate that IP-10 is associated with disease activity and perseverance of rheumatoid arthritis. PMID: 28592626
    26. CXCL10 in addition to IFN-gamma can be used to differentiate among Mycobacterium tuberculosis infection possibilities. PMID: 28610785
    27. Increased amniotic fluid CXCL10 concentration is associated with chronic chorioamnionitis or maternal anti-fetal rejection, whereas increased amniotic fluid IL-6 concentration is associated with acute histologic chorioamnionitis. PMID: 28544362
    28. Cord blood CXCL10 levels were negatively associated with mite sensitization at age 3. A high cord blood CCL22/CXCL10 chemokine ratio was significantly associated with a higher risk of allergic sensitization at age 3. PMID: 27863395
    29. CXCL10/IP-10 transcript showed up to 20 fold-increase, with similar changes detectable at the protein level in melanoma cells overexpressing PDFGRA. PMID: 27764787
    30. this study suggests that GG genotype of CXCL10 -135G/A (rs56061981) polymorphism decreased CXCL10 expression in T cells which may have defective recruitment of mononuclear cells at the site of infection as well granuloma formation and in turn contribute to progression of tuberculosis PMID: 28336310
    31. The regular early post-transplantation monitoring of urinary miR-155-5p and CXCL10 can help in the prognosis of acute rejection and graft dysfunction after kidney transplantation. PMID: 28880456
    32. gene polymorphisms of IL-8(-251T/A) and IP-10(-1596C/T) are associated with susceptibility and progression of type 2 diabetic retinopathy in a northern Chinese population PMID: 27935598
    33. Higher serum levels of CXCL10 are found in patients with non-segmental vitiligo (NSV) and NSV + autoimmune thyroiditis than in controls. PMID: 28698095
    34. results indicated that CXCL10, a pro-inflammatory chemokine, might be involved in the abnormal immune response in aplastic anaemia. PMID: 28411045
    35. IP-10 low in the tumor microenvironment can be used as potential indicators for the progression of non-small cell lung cancer PMID: 28375674
    36. The serum IP10 concentrations increase in women with polycystic ovary syndrome (PCOS), moreover, IP10 appears to be correlated with the inflammatory and Insulin resistance statuses of PCOS. IP10 may be a potential biomarker to estimate the disease activity of PCOS. PMID: 28051885
    37. EGF and IP-10 were significantly elevated and GRO levels were lower in the tear profile of HIV patients with dry eye disease (DED) compared to immunocompetent patients with DED. PMID: 27585367
    38. Determination of serum IP-10 levels before treatment could be useful for predicting favorable virologic response to TVR-based triple therapy, especially in patients with IL28B non-TT genotype. PMID: 27541605
    39. In lipotoxic hepatocytes, MLK3 activates a MAPK signaling cascade, resulting in the activating phosphorylation of STAT1, and CXCL10 transcriptional upregulation. PMID: 28262979
    40. Neuroendocrine-like cells promote the chemotaxis activity of tumor-associated macrophages (TAM) via CXCL10 and CXCL11. PMID: 27034164
    41. These results showed that the higher levels of CXCL10, CCL20 and CCL22 were associated with ischemic heart disease. The serum levels of chemokines may influence by the certain traditional risk factors of IHD and some studied SNPs, but did not influence by treatment and gender of patients. PMID: 27152707
    42. High CXCL10 expression is associated with clear-cell renal cell carcinoma. PMID: 26910919
    43. a massive and selective serum CXCL10 response in R. conorii-infected patients, likely reflecting release from infected endothelial cells characterized by infiltrating T cells and monocytes. PMID: 27180202
    44. The strongest OR was for CXCL8 (interleukin-8) in serum (96.8, 95% CI: 11.9-790.2). Of these 15 markers, 6 were also significantly elevated in serum from Chile (CCL20, C-reactive protein, CXCL8, CXCL10, resistin, serum amyloid A). PMID: 27173614
    45. These findings elucidate an NFAT-MAPK signaling paradigm for induction of isletokine expression in beta-cells and reveal IP-10 as a primary therapeutic target to prevent beta-cell-induced inflammatory loss of graft function after islet cell transplantation. PMID: 28855240
    46. High urine CXCL10 level is associated with acute rejection in Histologically Stable Kidney transplant Recipients. PMID: 26694099
    47. IL-10, IL-17 and IP-10 response to tubercular antigens act as potent discriminative markers for active tuberculosis among pulmonary tuberculosis suspects. PMID: 27450011
    48. The performance of IFNG release assays is robust despite variations in the incubation temperature between 37 degrees C and 39 degrees C for the diagnosis of latent tuberculosis infection. PMID: 27156612
    49. A novel function of CXCL10 in mediating monocyte production of proinflammatory cytokines in inflammatory bowel diseases has been described. PMID: 28899907
    50. CXCL10 is a circulating inflammatory marker elevated in advanced heart failure. PMID: 27271043

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine alpha (chemokine CxC) family
  • 组织特异性：
    Mainly secreted by monocytes, endothelial cells as well as fibroblasts. Expressed by epithelial cells in thymus. Microglial cells produce CXCL10 in response to viral stimulation.
  • 数据库链接：

    HGNC: 10637

    OMIM: 147310

    KEGG: hsa:3627

    STRING: 9606.ENSP00000305651

    UniGene: Hs.632586