Human histatin 5 ELISA Kit

产品详情

文献引用 (1) 说明书下载

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

This Human histatin-5 ELISA Kit was designed for the quantitative measurement of Human histatin-5 protein in serum, plasma, saliva. It is a Sandwich ELISA kit, its detection range is 12.5 ng/mL-800 ng/mL and the sensitivity is 3.12 ng/mL .
别名：

N/A
缩写：

histatin-5
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, saliva
检测范围：

12.5 ng/mL-800 ng/mL
灵敏度：

3.12 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Biochemicals
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Histatin 5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-95
1:2	Average %	94
	Range %	90-98
1:4	Average %	100
	Range %	95-104
1:8	Average %	102
	Range %	96-108

回收率：

The recovery of human Histatin 5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	97-103
EDTA plasma (n=4)	95	91-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.