Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Human fetal hemoglobin,HBF ELISA Kit

Human fetal hemoglobin,HBF ELISA Kit

  • 中文名称：
    人胎儿血红蛋白(HBF)酶联免疫试剂盒
  • 货号：
    CSB-E09598h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human HBF ELISA Kit was designed for the quantitative measurement of Human HBF protein in serum, plasma, lysate for RBC. It is a Competitive ELISA kit, its detection range is 125 ng/mL-2000 ng/mL and the sensitivity is 62.5 ng/mL.

  • 缩写：
    HBF
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, lysate for RBC
  • 检测范围：
    125 ng/mL-2000 ng/mL
  • 灵敏度：
    62.5 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human HBF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:20 Average % 95  
    Range % 91-99  
    1:40 Average % 103  
    Range % 99-107  
    1:80 Average % 98  
    Range % 93-103  
    1:160 Average % 92  
    Range % 87-97  
  • 回收率：
    The recovery of human HBF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 95-104  
    EDTA plasma (n=4) 89 84-94  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    2000 0.091 0.098 0.095    
    1000 0.175 0.171 0.173    
    500 0.301 0.305 0.303    
    250 0.599 0.596 0.598    
    125 1.265 1.211 1.238    
    0 2.244 2.201 2.223    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价