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  4. Human eosinophil cationic protein,ECP ELISA Kit

Human eosinophil cationic protein,ECP ELISA Kit

  • 中文名称：
    人嗜酸性粒细胞阳离子蛋白(ECP)酶联免疫试剂盒
  • 货号：
    CSB-E11729h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human ECP ELISA Kit was designed for the quantitative measurement of Human ECP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

  • 别名：
    Cytotoxic ribonuclease ELISA Kit; ECP ELISA Kit; ECP_HUMAN ELISA Kit; Eosinophil cationic protein ELISA Kit; OTTHUMP00000164017 ELISA Kit; Ribonuclease 3 ELISA Kit; Ribonuclease; RNase A family; 3 ELISA Kit; RNase 3 ELISA Kit; RNASE3 ELISA Kit; RNS3 ELISA Kit
  • 缩写：
    ECP
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.56 ng/mL-100 ng/mL
  • 灵敏度：
    0.39 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ECP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %93
    Range %88-98
    1:2Average %97
    Range %92-103
    1:4Average %100
    Range %94-108
    1:8Average %108
    Range %103-112
  • 回收率：
    The recovery of human ECP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9087-95
    EDTA plasma (n=4)9289-95
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    1002.500 2.493 2.497 2.392
    502.003 1.998 2.001 1.896
    251.275 1.266 1.271 1.166
    12.50.587 0.573 0.580 0.475
    6.250.369 0.357 0.363 0.258
    3.120.221 0.208 0.215 0.110
    1.560.176 0.169 0.173 0.068
    00.108 0.102 0.105 　
  • 本试剂盒所含材料：
    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human ECP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled ECP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

问答及客户评论

■ 常见问题解答
Q:

I am interested in your Human eosinophil Cationic protein, ECP ELISA Kit (CSB-E11729h). However, I will test other markers and need advide for collection/preparation for this kit as stated below:
“I am hoping to get some information about the collection/preparation of serum prior to analysis. My lab will be analyzing serum samples for a variety of markers. the other markers all state to allow clotting for 30 minutes prior to centrifugation. The ECP (human) kit that I am interested in states to allow clotting for 2 hours. I was wondering if you could tell me if it needs to sit for two hours, or if 30 minutes if suffice. We are trying to avoid needed to process two separate samples so i'm hoping you can help me!”

A:
Thanks for your inquiry.
CSB-E11729h
This kit can test serum and plasma samples. For serum samples, usually we will keep it static for 2 hours and then collect it. In this way more samples can be precipitated. What's more, if the static time is enough, the fibrin can timely trap hematocyte and then shrink and sink. Then if we treat it by centrifugation, the fibrin will not stay in the serum.
As it is mentioned, static for 30 minutes, we think that this static time is not very sufficient, and coagulation phenomenon is not very obvious. It is recommended to extend the static time. Later you should be able to see obvious coagulation and then extend the time. In this way more samples can be precipitated.
Pls let me know if you have any further questions. Thank you.

靶点详情

  • 功能：
    Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.
  • 基因功能参考文献：
    1. measuring serum ECP levels is helpful for assessing the efficacy of inhaled corticosteroids therapy in bronchial asthma children. PMID: 28781336
    2. Data suggest that radioactive I(131) therapy leads to down-regulation of secretion of ECP from Th2 cells (helper-inducer T-lymphocytes) in post-thyroidectomy patients treated for differentiated thyroid cancer; in this study, ECP levels in serum were analyzed before, 3 days after, and 7 days after radiotherapy with I(131). PMID: 29066479
    3. our data add new insights into the pathophysiology of AD, suggesting that BDNF which correlates with disease severity and ECP levels contributes to the regulation of inflammation in an eosinophil-, but not Th2-dependent manner. PMID: 27087278
    4. High circulating levels of eosinophil cationic protein are associated with increased incidence of ischemic stroke. PMID: 28904229
    5. Studies mapped the structural determinants required for the eosinophil cationic protein (ECP)-bacterial lipopolysaccharide (LPS) interaction by nuclear magnetic resonance. PMID: 27696685
    6. Increased MRP8/14 levels suggest the instability of the atherosclerotic plaque. ECP reflects the severity of coronary arteries stenosis, predicting atherosclerosis burden. They may become the new biomarkers of CAD. PMID: 27022611
    7. Children in the acute-phase group showed higher ECP mRNA expression level than those in the stable-phase and healthy control groups, while the level in the stable-phase was markedly higher than that in the healthy control group. PMID: 26600485
    8. drug eluting stent polymers increase eosinophil cationic protein levels following percutaneous coronary intervention independently of C-reactive protein PMID: 25463127
    9. Around half of all variance in serum ECP is explained by genetic factors. Serum ECP is influenced by sex, BMI, and airway responsiveness. Serum ECP and airway responsiveness seem not to share genetic variance. PMID: 25354326
    10. eosinophil cationic protein levels can be used to diagnosis atopic dermatitis but cannot distinguish between intrinsic and extrinsic disease. PMID: 24129707
    11. The antimicrobial protein, Rnase 3, contributes to the host immune response against mycobacteria. PMID: 23716047
    12. ECP was elevated in nasal discharge of patients with nasal polyps compared to controls and those with allergic rhinitis or rhinosinusitis. PMID: 23430080
    13. report the prevalence of a common SNP in the eosinophil protein x/eosinophil-derived neurotoxin (EPX/EDN, RNase2) and the association with the cellular contents of EPX/EDN and ECP PMID: 24738159
    14. Eosinophil cationic protein is not cytotoxic but enhances the growth of normal human dermal fibroblasts PMID: 24383969
    15. Effects of omalizumab on eosinophil cationic peptide, 25-hydroxyvitamin-D, IL-1beta and sCD200 in cases of Samter's syndrome PMID: 23841472
    16. Eosinophil activation occurred in early and late responses after L-ASA NPT in upper airway mucosa of AERD patients, where ApoA1, alpha2M and CP as well as CysLT may be involved in eosinophilic inflammation. PMID: 23786280
    17. Eosinophil cationic protein is a major granule-derived protein with cytotoxic activity found in eosinophils and has been known as a useful marker of allergic inflammation. PMID: 23710948
    18. Polymorphisms of EPX and ECP are associated to inflammatory bowel disease in an age and gender dependent manne. PMID: 23197886
    19. novel insights to identify functional regions of hRNase3 that may have implications for all hRNaseA superfamily members. PMID: 23484086
    20. This study also suggests that eosinophils and eosinophil cationic protein take part in the pathogenesis of nonallergic rhinitis. PMID: 23406588
    21. Lower ECP levels in the active phase of the disease may be a result of decreased production due to the activation of Th1 cytokines. PMID: 23517396
    22. A fraction of native ECP is stored in a non-cytotoxic form, which can be converted into a cytotoxic form by N-deglycosylation, whereas another fraction is stored as a highly cytotoxic form carrying different post-translational modifications PMID: 23597768
    23. Eosinophil cationic protein might induce mesoderm differentiation through fibroblast growth factor signaling pathway and enhance subsequent cardiomyocyte differentiation in concert with dimethyl sulfoxide in P19CL6 cells. PMID: 22845717
    24. a marker of asthma severity within an asthmatic paediatric population PMID: 21962901
    25. Results reveal the structural basis for the high affinity of ECP for glycosaminoglycans. PMID: 22579681
    26. biological and genetic characteristics of ECP PMID: 22845733
    27. 371G allele of RNASE3 is associated with susceptibility to cerebral malaria and forms part of a risk associated haplotype GGA defined by the markers PMID: 22216286
    28. Total serum IgE, ECP and eosinophil percentage are strong predictors of allergic rhinitis. PMID: 22289536
    29. The data suggests presence of relationship between development of restenosis and elevated blood plasma Eosinophilic cation protein level in patients with IHD after revascularization. PMID: 21878071
    30. ECP heparin binding affinity depends on its RNase catalytic site PMID: 20213669
    31. basal ECP levels are associated with major advemse cardiac evemts after bare metal stent implantation, suggesting that an allergic-mediated inflammation against the metal could explain some adverse reactions occurring after coronary stenting. PMID: 21183182
    32. Arg22, Arg34, Arg61, Arg77 and His64 of ECP are crucial for eosinophil cationic proteins's membrane destabilization activity, which appears to be the underlying mechanism of its cytotoxic, antibacterial and antiparasitic activities. PMID: 21303303
    33. The expression level of ECP mRNA was up-regulated in nasal polyps. PMID: 19086659
    34. the shortest ECP minimized version that would retain its antimicrobial properties PMID: 20951760
    35. ECP is present in the middle ear effusion of otitis media of effusion. There was a positive correlation between ECP level and IgE level. PMID: 16883790
    36. Among mite-allergic patients T-bet expression was down-regulated, which had no relation to ECP concentration and allergic symptoms. PMID: 16874959
    37. elevated levels in nasal lavage in allergic asthmatics PMID: 21038779
    38. Eosinophil cationic protein may have a role in coronary atherosclerosis PMID: 20307883
    39. There are associations of symptoms of allergy and asthma to ECP-genotypes, but also to smoking habits. ECP may be involved in impairment of lung functions in disease. PMID: 20534163
    40. Serum levels of ECP, IL-5, -6, -8 and -10, G-CSF, MCP-1, IL-1 ra and IP-10 were significantly elevated in acute compared with stable childhhood asthma. PMID: 20523065
    41. Results showed that OSCC patients with intense TATE and at least one C allele had a higher frequency of bilateral neck dissection, local recurrence, vascular embolization, involved resection margins, and postoperative radiotherapy. PMID: 19566743
    42. Our study suggests that RNase3 polymorphisms are potentially associated with susceptibility to allergic rhinitis. PMID: 19760211
    43. The eosinophil cationic protein increased TNF-alpha production in BEAS-2B cells and triggered apoptosis by caspase-8 activation through mitochondria-independent pathway. PMID: 20089176
    44. The mean of serum ECP was significantly higher in patients with respiratory tract diseases (asthma, COPD, interstitial lung disease, and respiratory infections) compared with healthy volunteers. PMID: 20170318
    45. Data show that conformational diversity of ECP is observed for the loop segments 17-22, 65-68, and 92-95 and most exposed sidechains. PMID: 19189375
    46. Measurement of ECP polymorphism is a significant indicator for the disease activity in asthmatic patient. PMID: 12524255
    47. serum levels elevated in asthmatic patients during acute attack PMID: 12530121
    48. results suggest a differential release of ECP by the serum eosinophil, depending on the disease status and asthma exacerbation severity PMID: 12974194
    49. Presence of ECP in human milk is associated with development of cow milk allergy and atopic dermatitis in the breast-fed infant, but has no direct association with the maternal atopy. PMID: 14630983
    50. ECP contributes to surfactant dysfunction in asthma, which in turn could lead to airway obstruction. PMID: 15007353

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  • 亚细胞定位：
    Secreted. Note=Located in the matrix of eosinophil large specific granule, which are released following activation by an immune stimulus.
  • 蛋白家族：
    Pancreatic ribonuclease family
  • 数据库链接：

    HGNC: 10046

    OMIM: 131398

    KEGG: hsa:6037

    STRING: 9606.ENSP00000302324

    UniGene: Hs.73839