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  4. Human endothelial cell-specific molecule 1(ESM-1) ELISA Kit

Human endothelial cell-specific molecule 1(ESM-1) ELISA Kit

  • 中文名称：
    人内皮细胞特异分子-1(ESM-1)酶联免疫试剂盒
  • 货号：
    CSB-E16530h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The Human endothelial cell-specific molecule 1 (ESM1) ELISA kit enables quantification of human DEFA1 in solution, e.g. in serum, plasma, and tissue homogenates. The product is more flexible to set up the ELISA assay. ESM1, also known as endocan, is a dermatan sulphate proteoglycan expressed and secreted primarily by the endothelial cells in the lung and kidneys. ESM1 expression in endothelial and epithelial cells is upregulated by TNF-α, IL-1β, and VEGF, but downregulated by IL-4 and IFN-γ. Plasma ESM1 levels are increased in patients with inflammatory disorders like sepsis, suggesting that ESM1 could be a potential biomarker for endothelial activation or dysfunction. EMS1 is frequently overexpressed in many cancer types and is related to tumor growth and angiogenesis during tumor progression. Tung-Wei Hung etc. proposed that overexpression of ESM1 promotes cell migration and renal fibrosis progression by modulating the endothelial-to-mesenchymal transition (EndoMT) pathways in vitro and in vivo.

    The assay is based on the Sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The intensity of the color is proportional to the ESM1 content in the sample. This kit has been verified with nanogram-level of sensitivity, high specificity, precision, good linearity, high recovery, and lot-to-lot consistency. More validation details are described in the product instructions.

  • 别名：
    Endocan ELISA Kit; Endothelial cell specific molecule 1 ELISA Kit; Endothelial cell-specific molecule 1 ELISA Kit; ESM 1 ELISA Kit; ESM 1 secretory protein ELISA Kit; ESM-1 ELISA Kit; Esm1 ELISA Kit; ESM1 secretory protein ELISA Kit; ESM1_HUMAN ELISA Kit
  • 缩写：
    ESM1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenatess.
  • 检测范围：
    3.12 ng/ml-200 ng/ml.
  • 灵敏度：
    0.78 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Tags & Cell Markers
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 本试剂盒所含材料：
      • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human ESM1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled ESM1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Involved in angiogenesis; promotes angiogenic sprouting. May have potent implications in lung endothelial cell-leukocyte interactions.
  • 基因功能参考文献：
    1. Results show that aerum ESM-1 levels were significantly higher in the idiopathic sudden sensorineural hearing loss group than the control group which may confirm endothelial dysfunction involvement. PMID: 30370871
    2. suggest that measurement of endocan level may help clinicians to predict the development of coronary collateral circulation in patients with stable coronary artery disease PMID: 28393589
    3. suggest that serum ESM-1 level may be a novel endothelial dysfunction biomarker PMID: 26927690
    4. Plasma ESM-1 levels are decreased during pregnancy and increased in early- and late-onset preeclampsia. The source of ESM-1 is probably not the placenta, but most likely maternal endothelial cells. PMID: 29674200
    5. Serial endocan concentration meets the major requirements for predicting outcome in patients with severe sepsis. An assay of endocan concentration may be a good prognostic biomarker in the clinic for severe sepsis. PMID: 29750963
    6. The cutoff serum level of endocan predicting AD was >288.94 ng/mL in receiver operating characteristic curve analysis PMID: 29393258
    7. A strong negative correlation was found between serum endocan levels and peak systolic velocity in men with severe erectile dysfunction. PMID: 29057489
    8. Results show the endocan level in peripheral blood significantly elevated in patients newly diagnosed with multiple myeloma (MM), and associated with myeloma-specific clinical parameters. PMID: 30194153
    9. The correlation between the secretion of endocan and that of IL-6 by endothelial cells suggests that endocan can be used as a predictive marker of inflammation for the quality assessment of transfusion grade PLTs PMID: 29238987
    10. Serum ESM-1 levels correlate with stress hyperglycemia in patients with STEMI and are associated with short-term prognosis. PMID: 26685180
    11. Endocan could be a common predictor of the endothelium-dependent inflammatory processes, rather than related with specific risk factors in coronary artery disease and microvascular angina. PMID: 26744512
    12. Endocan is an inflammatory marker released by the endothelium and is associated with endothelial dysfunction and atherosclerosis in patients with systemic lupus erythematosus. PMID: 26614790
    13. Endocan levels are higher in patients with large-artery atherosclerotic stroke and can help in predicting the short-term unfavourable outcome. PMID: 29287901
    14. elevated expression on epidermal keratinocytes and dermal endothelial cells in lesional skin of atopic dermatitis; decreased expression in atopic dermatitis sera PMID: 28714079
    15. Plasma ESM-1 is a novel biomarker for predicting the T status in OSCC patients. PMID: 29104463
    16. ESM1 expression correlates with Gleason grade/score and with androgen receptor expression in prostate carcinoma tissue, so ESM1 should also be considered as a biomarker for diagnosis of prostate cancer. PMID: 29104483
    17. Demonstrate an increased Endocan serum level in NAFLD patients, higher in those with type 2 diabetes mellitus and/or NASH because of an endothelial dysfunction in these pathologies. PMID: 28922438
    18. Studied serum endocan levels in endometrial and ovarian cancer. Serum endocan levels were significantly higher in the entire patient group than in the controls. PMID: 27734523
    19. This study showed that ESM-1 level in pleural effusion could be a potential diagnostic and prognostic marker in NSCLC patients with MPE. PMID: 28514746
    20. Severe erectile dysfunction was associated with higher serum endocan levels than less severe erectile dysfunction or healthy controls. PMID: 28424502
    21. Serum endocan levels >/= 2.05 ng/ml had a sensitivity of 76.1% and specificity of 85% for the diagnosis of bacterial infection in decompensated cirrhotic patients. The endocan measured at admission is a good clinical parameter predicting the occurrence of infection in these patients. Elevated endocan may reflect the degree of endothelial cell injury by a systemic inflammatory response. PMID: 28614777
    22. study showed that higher endocan levels were significantly and independently related to the presence of slow coronary flow. PMID: 26607436
    23. The results suggest that endocan may have a role in the pathogenesis of pterygium. PMID: 28410547
    24. Endocan expression is increased in the human placenta from obese women with gestational diabetes, and in response to pro-inflammatory stimuli. Loss-of-function studies in villous trophoblast cells and HUVECs revealed that endocan is not directly involved in the genesis or in the expression of pro-inflammatory cytokines induced by LPS or IL-1beta. PMID: 27871471
    25. Systemic inflammation may be responsible for the rise in endocan levels following cardiopulmonary bypass. PMID: 26999704
    26. Endocan expression was correlated with poor clinical outcomes in patients with Pancreatic Neuroendocrine Tumors . The results indicated that endocan expression may be a reliable marker for predicting tumor recurrence in patients with PNETs. PMID: 29019897
    27. glomerular-derived Esm-1 as a potential non-invasive biomarker of diabetic nephropathy PMID: 28934365
    28. Physical activity increases circulating endocan level. PMID: 28056805
    29. These findings provide the first evidence that the imbalance of MMP-9/TIMP-1, is one of the regulation mechanisms by which ESM1 promotes tumorigenicity and metastasis of prostate cancer cells. PMID: 28108731
    30. These results are consistent with the view that endocan measured in ICU patients is intact, stable, and accurate. Then, the low endocan level observed in ICU patients who developed acute respiratory distress syndrome is likely to be reliable. PMID: 28427918
    31. Dopamine agonist resistance-related ESM1 promotes the angiogenesis and tumor cells growth of prolactinomas, suggesting that ESM1 may be a novel therapeutic target for prolactinomas. PMID: 26662185
    32. These results suggest human cytomegalovirus infection leads to glioma progression through an upregulation of endocan and the secretion of inflammatory cytokines. PMID: 27474433
    33. Endocan-expressing microvessel density significantly correlates with the expression of VEGF and VEGFR2 and is a valuable prognostic factor for survival in human gastric cancer. PMID: 27340359
    34. Circulating P-selectin levels were closely associated with endocan levels in obese children and adolescents with non-alcoholic fatty liver disease. PMID: 28318371
    35. this study shows that endocan may play an important role in the development of connective tissue disease related pulmonary arterial hypertension PMID: 27912147
    36. The distribution of Gleason score and biochemical failure rate were significantly higher in patients with serum endocan >/=1.8 ng/mL (p = 0.031 and p = 0.047). PMID: 27470607
    37. endocan plays an anti-carcinogenic role in gastric cancer development and progression and might serve as a prognostic biomarker as well as a potential therapeutic target for gastric cancer. PMID: 27644250
    38. High ESM1 expression is associated with Meningiomas and Gliomas. PMID: 27528791
    39. Serum and urinary endocan concentrations are significantly elevated in patients with bladder cancer. PMID: 26748103
    40. Subclinical hypothyroidism is associated with increased levels of serum endocan, dimethylarginine, and TGF-beta, which are new markers for endothelial dysfunction. PMID: 27993872
    41. The levels of ESM1, CLDN5, IL-1beta, IL-6, and TNF-alpha were significantly higher in the migraine attack group than in the control group PMID: 27010153
    42. Results found that serum endocan levels were significantly lower in Alzheimer's disease (AD) patients with depression than in AD patients without depression or healthy controls suggesting that serum endocan levels might be associated with depression in AD. PMID: 26924874
    43. serum levels of endocan, a new biomarker of endothelial dysfunction, increase in obstructive sleep apnea subjects. PMID: 26808694
    44. Endocan may play a role in the progression of pregnancy and has a potential to be a new marker for the detection of pre-eclampsia PMID: 26823798
    45. Serum endocan concentrations were significantly elevated in women with pre-eclampsia versus normotensive controls, and concentrations seem to be associated with the severity of the disease. PMID: 26418319
    46. Suggest that serum endocan concentrations are elevated in patients with type 2 diabetes and decrease following anti-hyperglycemic treatment. PMID: 25969567
    47. Median maternal plasma endocan concentrations were higher in preeclampsia and lower in acute pyelonephritis with bacteremia than in uncomplicated pregnancy. PMID: 25211383
    48. Elevated endocan levels were more strongly associated with disease severity among Johnson Syndrome, Toxic Epidermal Necrolysis subjects than among less severe allergic reactions with skin involvement. PMID: 25394955
    49. Detection of ESM-1 levels in the serum may serve as a tumor biomarker for assessing prognosis in colorectal cancer. PMID: 26125749
    50. Serum endocan level is independently correlated with the presence and severity of coronary artery disease in hypertension patients. PMID: 26200037

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  • 亚细胞定位：
    Secreted.
  • 组织特异性：
    Expressed in lung, on the vascular capillary network within alveolar walls, and also at lower level in kidney.
  • 数据库链接：

    HGNC: 3466

    OMIM: 601521

    KEGG: hsa:11082

    STRING: 9606.ENSP00000370812

    UniGene: Hs.129944