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  4. Human dermatopontin,DPT ELISA Kit

Human dermatopontin,DPT ELISA Kit

  • 中文名称：
    人皮肤蛋白(DPT)酶联免疫试剂盒
  • 货号：
    CSB-E11277h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human DPT ELISA Kit was designed for the quantitative measurement of Human DPT protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

  • 别名：
    DERM_HUMAN ELISA Kit; Dermatopontin ELISA Kit; Dpt ELISA Kit; TRAMP ELISA Kit; Tyrosine rich acidic matrix protein ELISA Kit; Tyrosine-rich acidic matrix protein ELISA Kit
  • 缩写：
    DPT
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human DPT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %92
    Range %85-102
    1:2Average %91
    Range %82-103
    1:4Average %95
    Range %86-108
    1:8Average %94
    Range %87-105
  • 回收率：
    The recovery of human DPT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9583-104
    EDTA plasma (n=4)9587-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.417 2.493 2.455 2.314
    101.763 1.817 1.790 1.649
    51.025 0.982 1.004 0.863
    2.50.611 0.595 0.603 0.462
    1.250.436 0.416 0.426 0.285
    0.6250.329 0.307 0.318 0.177
    0.3120.238 0.228 0.233 0.092
    00.142 0.139 0.141
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Seems to mediate adhesion by cell surface integrin binding. May serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. Enhances TGFB1 activity. Inhibits cell proliferation. Accelerates collagen fibril formation, and stabilizes collagen fibrils against low-temperature dissociation.
  • 基因功能参考文献：
    1. DPT gene silencing could effectively reduce the proliferation of MG-63 cells, slow down the cell cycle progression and promote cell apoptosis. PMID: 29115446
    2. Novel mutation in DPT segregates with the complete phenotype with variable expressivity in two pedigrees with autosomal dominant familial Meniere's disease. PMID: 27876815
    3. This study essentially deals with assessing the expression and integrity of an extracellular matrix protein, Dermatopontin (DPT), in chronic cutaneous wounds. PMID: 25260909
    4. dermatopontin functional peptide produces fibrinogen fibrils and modifies its biological activity PMID: 25082449
    5. These results indicate that DPT can modify certain biological functions of fibrin, and thus a another function of this extracellular matrix protein was revealed. PMID: 23877568
    6. data provided strong evidence that downregulation of DPT is a characteristic event in human oral squamous cell carcinoma and that DPT was correlated with cellular adhesion and invasiveness. PMID: 21796630
    7. DPT has an accelerating role in fibroblast cell adhesion to the provisional matrix in the initial stage of wound healing. PMID: 21398523
    8. suggests that dermatopontin can play various roles in different tissues and might be a molecule related to carcinogenesis and the progression of HCC via possible interaction with TGF-beta1 and other potential mechanisms PMID: 19916908
    9. roles of dermatopontin and histamine receptor H1 genes as downstream targets for the VDR were confirmed by gel electromotility shift and chromatin immunoprecipitation assays that showed the presence of VDR complex binding sequences PMID: 17547532

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Dermatopontin family
  • 组织特异性：
    Expressed in fibroblasts, heart, skeletal muscle, brain and pancreas. Expressed at an intermediate level in lung and kidney, and at a low level in liver and placenta. Expressed at a lower level in fibroblasts from hypertrophic scar lesional skin and in fi
  • 数据库链接：

    HGNC: 3011

    OMIM: 125597

    KEGG: hsa:1805

    STRING: 9606.ENSP00000356791

    UniGene: Hs.80552