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  4. Human cytokeratin fragment antigen 21-1,CYFRA21-1 ELISA Kit

Human cytokeratin fragment antigen 21-1,CYFRA21-1 ELISA Kit

  • 中文名称：
    人细胞角蛋白21-1片段(CYFRA21-1)酶联免疫试剂盒
  • 货号：
    CSB-E09606h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human CYFRA21-1 ELISA Kit was designed for the quantitative measurement of Human CYFRA21-1 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .

  • 缩写：
    CYFRA21-1
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CYFRA21-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %82-90
    1:2Average %98
    Range %91-105
    1:4Average %99
    Range %94-110
    1:8Average %92
    Range %86-99
  • 回收率：
    The recovery of human CYFRA21-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8980-98
    EDTA plasma (n=4)9790-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.541 2.385 2.463 2.305
    102.229 2.082 2.156 1.998
    51.607 1.545 1.576 1.418
    2.50.866 0.842 0.854 0.696
    1.250.463 0.439 0.451 0.293
    0.6250.337 0.324 0.331 0.173
    0.3120.254 0.246 0.250 0.092
    00.160 0.156 0.158 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

May I know are the antibodies used in the ELISA kit polyclonal antibodies or monoclonal antibodies?
Human cytokeratin fragment antigen 21-1 (CYFRA21-1) ELISA Kit

A:
Thanks for your inquiry.Pls check the details as below:
Capture:mouse monoclonal antibody;Detection:goat polyclonal antibody
If further questions,pls let me know.