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  4. Human cyclophilin B,CyPB ELISA Kit

Human cyclophilin B,CyPB ELISA Kit

  • 中文名称：
    人嗜环蛋白/亲环素B(CyPB)酶联免疫试剂盒
  • 货号：
    CSB-E11218h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    The human cyclophilin B (CyPB) ELISA Kit is used to quantitatively measure CyPB concentrations in human serum, plasma, cell lysates, and tissue homogenates. It performs well in important characteristics, including sensitivity, specificity, precision, recovery, linearity, and lot-to-lot consistency. This assay is based on the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops proportionally to the amount of CyPB in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.

    CyPB, also known as PPIB, is an immunophilin with cis-trans peptidyl-prolyl isomerase (PPIase) and chaperone-like activities. It functions to protect cells against aldosterone-induced oxidative stress, endoplasmic reticulum stress (ERS), and tubular cell injury via its PPIase activity. Furthermore, CyPB also participates in transcriptional regulation, protein secretion, immune response, apoptosis, and other cellular processes. Many studies have shown that CyPB plays an important role in the replication of several viruses, including the Japanese encephalitis virus (JEV), hepatitis C virus (HCV), and human papillomavirus type 16 (HPV 16).

  • 别名：
    AA408962 ELISA Kit; AA553318 ELISA Kit; AI844835 ELISA Kit; Cphn 2 ELISA Kit; Cphn2 ELISA Kit; Cyclophilin B ELISA Kit; Cyclophilin like protein ELISA Kit; CyP 20b ELISA Kit; CYP S1 ELISA Kit; CYP-S1 ELISA Kit; CYPB ELISA Kit; EC 5.2.1.8 ELISA Kit; MGC14109 ELISA Kit; MGC2224 ELISA Kit; OI9 ELISA Kit; peptidyl prolyl cis trans isomerase B ELISA Kit; Peptidyl prolyl cis trans isomerase B precursor ELISA Kit; Peptidyl-prolyl cis-trans isomerase B ELISA Kit; peptidylprolyl isomerase B (cyclophilin B) ELISA Kit; Peptidylprolyl isomerase B ELISA Kit; PPIase ELISA Kit; PPIase B ELISA Kit; Ppib ELISA Kit; PPIB_HUMAN ELISA Kit; Rotamase ELISA Kit; Rotamase B ELISA Kit; S cyclophilin ELISA Kit; S-cyclophilin ELISA Kit; SCYLP ELISA Kit
  • 缩写：
    PPIB
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell lysates, tissue homogenates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CyPB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %84
    Range %80-92
    1:2Average %90
    Range %85-85
    1:4Average %92
    Range %88-98
    1:8Average %98
    Range %93-104
  • 回收率：
    The recovery of human CyPB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10095-109
    EDTA plasma (n=4)9993-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.545 2.402 2.474 2.359
    10001.732 1.846 1.789 1.674
    5001.187 1.164 1.176 1.061
    2500.604 0.639 0.622 0.507
    1250.392 0.374 0.383 0.268
    62.50.268 0.280 0.274 0.159
    31.250.201 0.208 0.205 0.090
    00.118 0.112 0.115
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding.
  • 基因功能参考文献：
    1. A graphene-based nanocarrier modified with death receptor 4 (DR4) antibody and AKT siRNA has been developed, which can synergistically strengthen death receptor-mediated apoptosis and enhance the cancer therapeutic effect in vivo. PMID: 30430148
    2. Data show that Cyclophilin B is present on the erythrocytes and binds strongly to merozoites. PMID: 29146974
    3. cyclophilin B (CYPB) functions in protecting cells against aldosterone-induced oxidative stress, endoplasmic reticulum stress (ERS) and tubular cell injury via its peptidyl-prolyl cis-trans isomerase (PPIase) activity. PMID: 27732567
    4. PPIB mutations and their associated phenotypes PMID: 28242392
    5. observed changes in activity of six rER-resident PPIases, cyclophilin B (encoded by the PPIB gene), FKBP13 (FKBP2), FKBP19 (FKBP11), FKBP22 (FKBP14), FKBP23 (FKBP7), and FKBP65 (FKBP10), due to posttranslational modifications of proline residues in the substrate. PMID: 28385890
    6. The data suggest that overexpressed CypB protects neuronal cells from MPP+-induced dopaminergic neuronal cell death PMID: 27569281
    7. This study enhances our knowledge about the mutational pattern of the LEPRE1, CRTAP, and PPIB genes. LEPRE1 should be the first gene analyzed in mutation detection studies in patients with recessive OI. PMID: 26634552
    8. Over-expression of CypB enhances HIV infection by increasing nuclear import of viral DNA. PMID: 26774171
    9. Cyclophilin B has a high diagnostic value for stomach cancer and its downregulation can effectively inhibit the growth of stomach cancer cells. Thus, cyclophilin B may be a potential therapeutic target for stomach cancer treatment PMID: 26125731
    10. The extracellular portion of cyclophilin B probably plays an important role in human diseases associated with acute or chronic inflammation PMID: 24713575
    11. Findings demonstrate that CypB prevents hypoxia-induced cell death through modulation of ubiquitin-mediated CHOP protein degradation, suggesting that CypB may have an important role in the tight regulation of CHOP under hypoxia. PMID: 24270407
    12. These findings establish cyclophilin C as an ER cyclophilin, demonstrate the novel involvement of cyclophilins B and C in ER redox homeostasis PMID: 24990953
    13. Cyclophilin B is a novel MDM2 interacting partner. PMID: 24583282
    14. cyclophilin B (CypB) is a prolyl isomerase residing in the endoplasmic reticulum (ER), that provides an essential survival signal in glioblastoma multiforme cells through myc and mutant p53 PMID: 24272483
    15. An additional function of the rough endoplasmic reticulum protein complex prolyl 3-hydroxylase 1.cartilage-associated protein.cyclophilin B: the CXXXC motif reveals disulfide isomerase activity in vitro. PMID: 24043621
    16. Cyclophilin B activity regulated secretion and activity of ADAMTS13. PMID: 23144461
    17. CypB has an essential function in protecting hepatoma cells against oxidative stress through binding to CD147 and regulating the ERK pathway PMID: 22555451
    18. these findings suggest an unexpected role for cyclophilin B in attenuation of the responses of proinflammatory macrophages PMID: 22798670
    19. CypB interacts with these proteins and is involved in ribosome biogenesis and RNA transcription. PMID: 22426501
    20. Cyclophilin B is a candidate pancreatic cancer biomarker. PMID: 22484812
    21. CypB induced by hypoxia stimulates the survival of hepatocellular carcinoma via a positive feedback loop with HIF-1alpha. PMID: 21748762
    22. CAHL is a CsA associated helicase-like protein, which would form trimer complex with cyclophilin B and NS5B of HCV PMID: 21559518
    23. peptidylprolyl cis-trans isomerase B may be required to effectively fold the proline-rich regions of the C-terminal propeptide of procollagen PMID: 21282188
    24. cyclophilin isoform B is likely responsible for down-regulation of carrier expression by CsA and that it does so via its chaperone activity on NaDC1 (by direct interaction) rather than its rotamase activity. PMID: 21257749
    25. These results suggest that CypB plays a crucial role in the replication of Japanese encephalitis virus through an interaction with NS4A. PMID: 21281954
    26. Data indicate that CypB through its interaction with Na/K-beta1 might regulate maturation and trafficking of the pump through the secretory pathway. PMID: 21085665
    27. Results reveal the P-domain functions as a unique protein-protein interaction domain and implicate a peptidyl prolyl isomerase as a new element in the calnexin cycle. PMID: 20801878
    28. Cyclophilin B significantly modulate prolactin-induced function in breast cancer cells, ultimately resulting in enhanced levels of prolactin-responsive gene expression, cell growth, and migration. PMID: 20237142
    29. a regulatory mechanism in which cell type-specific expression of certain heparan sulfate sulfotransferases determines the specific binding of CyPB to target cells PMID: 19940140
    30. calcium signaling through CD147 receptor; induces neutrophil chemotaxis PMID: 11688976
    31. act in the context of t lymphocyte recruitment and function PMID: 11867726
    32. Different regions of CyPB are involved in peripheral blood T-lymphocyte activation and imply an important physiological function for peptidylprolyl isomerase activity. PMID: 11955071
    33. Inhibition of the PT-pore (ANT-1) via up-regulation of cyclophilin D plays a role in tumorigenesis PMID: 14729611
    34. Cyclophilin B plays a critical role in the activation of interferon regulatory factor-3. PMID: 15764595
    35. CYPB is a functional rfegulator of hepacivirus RNA polymerase. PMID: 15989969
    36. results strongly support the hypothesis that 3-O-sulfation of GlcNH2 residues could be a key modification that provides specialized HS structures for CyPB binding to responsive cells PMID: 17588944
    37. Altogether, our results support a model whereby CyPB induces integrin-mediated adhesion via interaction with a multimolecular unit formed by the association between CD147, CD98 and beta1 integrins. PMID: 18054915
    38. CypB is a new TRPV6 accessory protein with potential involvement in TRPV6 channel activation through its peptidyl-prolyl cis/trans isomerase activity PMID: 18445599
    39. the enhanced expression of CypB in malignant breast epithelium may contribute to the pathogenesis of this disease PMID: 19056847
    40. PPIB mutations cause severe osteogenesis imperfecta PMID: 19781681

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  • 相关疾病：
    Osteogenesis imperfecta 9 (OI9)
  • 亚细胞定位：
    Virion.
  • 蛋白家族：
    Cyclophilin-type PPIase family, PPIase B subfamily
  • 数据库链接：

    HGNC: 9255

    OMIM: 123841

    KEGG: hsa:5479

    STRING: 9606.ENSP00000300026

    UniGene: Hs.434937